Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Live cell super-resolution imaging requires a high temporal resolution, which remains a challenge. Here the authors combine photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI) to achieve high spatiotemporal resolution and quantitative imaging of fo...

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Autores principales: Hendrik Deschout, Tomas Lukes, Azat Sharipov, Daniel Szlag, Lely Feletti, Wim Vandenberg, Peter Dedecker, Johan Hofkens, Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic
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Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/b60dbd46b55d452085ed56fc80de41ea
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spelling oai:doaj.org-article:b60dbd46b55d452085ed56fc80de41ea2021-12-02T14:40:15ZComplementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions10.1038/ncomms136932041-1723https://doaj.org/article/b60dbd46b55d452085ed56fc80de41ea2016-12-01T00:00:00Zhttps://doi.org/10.1038/ncomms13693https://doaj.org/toc/2041-1723Live cell super-resolution imaging requires a high temporal resolution, which remains a challenge. Here the authors combine photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI) to achieve high spatiotemporal resolution and quantitative imaging of focal adhesion dynamics.Hendrik DeschoutTomas LukesAzat SharipovDaniel SzlagLely FelettiWim VandenbergPeter DedeckerJohan HofkensMarcel LeuteneggerTheo LasserAleksandra RadenovicNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Hendrik Deschout
Tomas Lukes
Azat Sharipov
Daniel Szlag
Lely Feletti
Wim Vandenberg
Peter Dedecker
Johan Hofkens
Marcel Leutenegger
Theo Lasser
Aleksandra Radenovic
Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
description Live cell super-resolution imaging requires a high temporal resolution, which remains a challenge. Here the authors combine photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI) to achieve high spatiotemporal resolution and quantitative imaging of focal adhesion dynamics.
format article
author Hendrik Deschout
Tomas Lukes
Azat Sharipov
Daniel Szlag
Lely Feletti
Wim Vandenberg
Peter Dedecker
Johan Hofkens
Marcel Leutenegger
Theo Lasser
Aleksandra Radenovic
author_facet Hendrik Deschout
Tomas Lukes
Azat Sharipov
Daniel Szlag
Lely Feletti
Wim Vandenberg
Peter Dedecker
Johan Hofkens
Marcel Leutenegger
Theo Lasser
Aleksandra Radenovic
author_sort Hendrik Deschout
title Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
title_short Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
title_full Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
title_fullStr Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
title_full_unstemmed Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
title_sort complementarity of palm and sofi for super-resolution live-cell imaging of focal adhesions
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/b60dbd46b55d452085ed56fc80de41ea
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