Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions

Abstract Background The oral cavity represents a main entrance of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Angiotensin-converting enzyme 2 (ACE-2), neuropilin-1 (NRP-1), and transmembrane serine protease 2 (TMPRSS2) are essential for the entry of SARS-CoV-2 to the host cells...

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Autores principales:	Alaa Muayad Altaie, Rania Hamdy, Thenmozhi Venkatachalam, Rifat Hamoudi, Sameh S. M. Soliman
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Oral lesions Fatty acids SARS-CoV-2 COVID-19 Infection load Oleic acid Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/b611224fa8b4415c976c804718daf4bc
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spelling	oai:doaj.org-article:b611224fa8b4415c976c804718daf4bc2021-11-14T12:32:07ZEstimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions10.1186/s12903-021-01921-51472-6831https://doaj.org/article/b611224fa8b4415c976c804718daf4bc2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12903-021-01921-5https://doaj.org/toc/1472-6831Abstract Background The oral cavity represents a main entrance of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Angiotensin-converting enzyme 2 (ACE-2), neuropilin-1 (NRP-1), and transmembrane serine protease 2 (TMPRSS2) are essential for the entry of SARS-CoV-2 to the host cells. Both ACE-2 and NRP-1 receptors and TMPRSS2 have been identified in the oral cavity. However, there is limited knowledge about the impact of periapical lesions and their metabolites on the expression of these critical genes. This study aims to measure the impact of periapical lesions and their unique fatty acids (FAs) metabolites on the expression of the aforementioned genes, in addition to interleukin 6 (IL-6) gene and hence SARS-CoV-2 infection loads can be estimated. Methods Gene expression of ACE-2, NRP-1, TMPRSS2, and IL-6 was performed in periapical lesions in comparison to healthy oral cavity. Since FAs are important immunomodulators required for the lipid synthesis essential for receptors synthesis and viral replication, comparative FAs profiling was determined in oral lesions and healthy pulp tissues using gas chromatography–mass spectrometry (GC–MS). The effect of major identified and unique FAs was tested on mammalian cells known to express ACE-2, NRP-1, and TMPRSS2 genes. Results Gene expression analysis indicated that ACE-2, NRP-1, and TMPRSS2 were significantly upregulated in healthy clinical samples compared to oral lesions, while the reverse was true with IL-6 gene expression. Saturated and monounsaturated FAs were the major identified shared and unique FAs, respectively. Major shared FAs included palmitic, stearic and myristic acids with the highest percentage in the healthy oral cavity, while unique FAs included 17-octadecynoic acid in periapical abscess, petroselinic acid and l-lactic acid in periapical granuloma, and 1-nonadecene in the radicular cyst. Computational prediction showed that the binding affinity of identified FAs to ACE-2, TMPRSS2 and S protein were insignificant. Further, FA-treated mammalian cells showed significant overexpression of ACE-2, NRP-1 and TMPRSS2 genes except with l-lactic acid and oleic acid caused downregulation of NRP-1 gene, while 17-octadecynoic acid caused insignificant effect. Conclusion Collectively, a healthy oral cavity is more susceptible to viral infection when compared to that complicated with periapical lesions. FAs play important role in viral infection and their balance can affect the viral loads. Shifting the balance towards higher levels of palmitic, stearic and 1-nonadecene caused significant upregulation of the aforementioned genes and hence higher viral loads. On the other hand, there is a reverse correlation between inflammation and expression of SARS-CoV-2 receptors. Therefore, a mouth preparation that can reduce the levels of palmitic, stearic and 1-nonadecene, while maintaining an immunomodulatory effect can be employed as a future protection strategy against viral infection.Alaa Muayad AltaieRania HamdyThenmozhi VenkatachalamRifat HamoudiSameh S. M. SolimanBMCarticleOral lesionsFatty acidsSARS-CoV-2COVID-19Infection loadOleic acidDentistryRK1-715ENBMC Oral Health, Vol 21, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oral lesions Fatty acids SARS-CoV-2 COVID-19 Infection load Oleic acid Dentistry RK1-715
spellingShingle	Oral lesions Fatty acids SARS-CoV-2 COVID-19 Infection load Oleic acid Dentistry RK1-715 Alaa Muayad Altaie Rania Hamdy Thenmozhi Venkatachalam Rifat Hamoudi Sameh S. M. Soliman Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions
description	Abstract Background The oral cavity represents a main entrance of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Angiotensin-converting enzyme 2 (ACE-2), neuropilin-1 (NRP-1), and transmembrane serine protease 2 (TMPRSS2) are essential for the entry of SARS-CoV-2 to the host cells. Both ACE-2 and NRP-1 receptors and TMPRSS2 have been identified in the oral cavity. However, there is limited knowledge about the impact of periapical lesions and their metabolites on the expression of these critical genes. This study aims to measure the impact of periapical lesions and their unique fatty acids (FAs) metabolites on the expression of the aforementioned genes, in addition to interleukin 6 (IL-6) gene and hence SARS-CoV-2 infection loads can be estimated. Methods Gene expression of ACE-2, NRP-1, TMPRSS2, and IL-6 was performed in periapical lesions in comparison to healthy oral cavity. Since FAs are important immunomodulators required for the lipid synthesis essential for receptors synthesis and viral replication, comparative FAs profiling was determined in oral lesions and healthy pulp tissues using gas chromatography–mass spectrometry (GC–MS). The effect of major identified and unique FAs was tested on mammalian cells known to express ACE-2, NRP-1, and TMPRSS2 genes. Results Gene expression analysis indicated that ACE-2, NRP-1, and TMPRSS2 were significantly upregulated in healthy clinical samples compared to oral lesions, while the reverse was true with IL-6 gene expression. Saturated and monounsaturated FAs were the major identified shared and unique FAs, respectively. Major shared FAs included palmitic, stearic and myristic acids with the highest percentage in the healthy oral cavity, while unique FAs included 17-octadecynoic acid in periapical abscess, petroselinic acid and l-lactic acid in periapical granuloma, and 1-nonadecene in the radicular cyst. Computational prediction showed that the binding affinity of identified FAs to ACE-2, TMPRSS2 and S protein were insignificant. Further, FA-treated mammalian cells showed significant overexpression of ACE-2, NRP-1 and TMPRSS2 genes except with l-lactic acid and oleic acid caused downregulation of NRP-1 gene, while 17-octadecynoic acid caused insignificant effect. Conclusion Collectively, a healthy oral cavity is more susceptible to viral infection when compared to that complicated with periapical lesions. FAs play important role in viral infection and their balance can affect the viral loads. Shifting the balance towards higher levels of palmitic, stearic and 1-nonadecene caused significant upregulation of the aforementioned genes and hence higher viral loads. On the other hand, there is a reverse correlation between inflammation and expression of SARS-CoV-2 receptors. Therefore, a mouth preparation that can reduce the levels of palmitic, stearic and 1-nonadecene, while maintaining an immunomodulatory effect can be employed as a future protection strategy against viral infection.
format	article
author	Alaa Muayad Altaie Rania Hamdy Thenmozhi Venkatachalam Rifat Hamoudi Sameh S. M. Soliman
author_facet	Alaa Muayad Altaie Rania Hamdy Thenmozhi Venkatachalam Rifat Hamoudi Sameh S. M. Soliman
author_sort	Alaa Muayad Altaie
title	Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions
title_short	Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions
title_full	Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions
title_fullStr	Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions
title_full_unstemmed	Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions
title_sort	estimating the viral loads of sars-cov-2 in the oral cavity when complicated with periapical lesions
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b611224fa8b4415c976c804718daf4bc
work_keys_str_mv	AT alaamuayadaltaie estimatingtheviralloadsofsarscov2intheoralcavitywhencomplicatedwithperiapicallesions AT raniahamdy estimatingtheviralloadsofsarscov2intheoralcavitywhencomplicatedwithperiapicallesions AT thenmozhivenkatachalam estimatingtheviralloadsofsarscov2intheoralcavitywhencomplicatedwithperiapicallesions AT rifathamoudi estimatingtheviralloadsofsarscov2intheoralcavitywhencomplicatedwithperiapicallesions AT samehsmsoliman estimatingtheviralloadsofsarscov2intheoralcavitywhencomplicatedwithperiapicallesions
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Estimating the viral loads of SARS-CoV-2 in the oral cavity when complicated with periapical lesions

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