Novel chitinolytic bacteria from the shrimp shell processing waste

Abstract. Masri M, Sukmawaty E, Aditia L. 2021. Novel chitinolytic bacteria from the shrimp shell processing waste. Biodiversitas 22: 2672-2681. Chitinase is an enzyme that hydrolyzes chitin (?-1,4 Nsetyl-D-glucosamine polymer). Several types of microorganisms produce chitinase that contributes to b...

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Autores principales:	Mashuri Masri, EKA SUKMAWATY, LASINRANG ADITIA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	brevibacillus reuszeri chitinase enzyme lysinibacillus fusiformis shrimp shell waste of factory Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b61348bd94cc4cfdb2e39ea0ca0706ab
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spelling	oai:doaj.org-article:b61348bd94cc4cfdb2e39ea0ca0706ab2021-11-22T12:13:35ZNovel chitinolytic bacteria from the shrimp shell processing waste1412-033X2085-472210.13057/biodiv/d220527https://doaj.org/article/b61348bd94cc4cfdb2e39ea0ca0706ab2021-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8019https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Masri M, Sukmawaty E, Aditia L. 2021. Novel chitinolytic bacteria from the shrimp shell processing waste. Biodiversitas 22: 2672-2681. Chitinase is an enzyme that hydrolyzes chitin (?-1,4 Nsetyl-D-glucosamine polymer). Several types of microorganisms produce chitinase that contributes to basic science and application. The role of chitinase enzyme is widely utilized in various fields, such as health as an anti-tumor agent and agriculture as biological control agents in plant disease caused by fungus. The purpose of this study was to isolate novel chitinolytic bacteria from shrimp shell waste in Makassar, Indonesia, using the scatter plate method on chitin agar selective media incubated at 37°C for 3 days. The clear zone formation around the colony was marked as an indicator of chitinase activity performed by the certain isolate. Selection was carried out by recycling all isolates on chitin agar media, which were incubated at 37°C for 3 days and measured the chitinolytic index. Two of ten isolates produced the highest chitinolytic index, namely, KLA-2 (45) and KLA-4 (15) isolates. The molecular identification results showed that the KLA-2 and KLA-4 isolates were Lysinibacillus fusiformis strain WH22 and Brevibacillus reuszeri strain CMB-15, respectively. Therefore, novel chitinolytic bacteria that can produce chitinase enzyme from shrimp shell waste are L. fusiformis and B. reuszeriMashuri MasriEKA SUKMAWATYLASINRANG ADITIAMBI & UNS Soloarticlebrevibacillus reuszerichitinase enzymelysinibacillus fusiformisshrimp shell waste of factoryBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	brevibacillus reuszeri chitinase enzyme lysinibacillus fusiformis shrimp shell waste of factory Biology (General) QH301-705.5
spellingShingle	brevibacillus reuszeri chitinase enzyme lysinibacillus fusiformis shrimp shell waste of factory Biology (General) QH301-705.5 Mashuri Masri EKA SUKMAWATY LASINRANG ADITIA Novel chitinolytic bacteria from the shrimp shell processing waste
description	Abstract. Masri M, Sukmawaty E, Aditia L. 2021. Novel chitinolytic bacteria from the shrimp shell processing waste. Biodiversitas 22: 2672-2681. Chitinase is an enzyme that hydrolyzes chitin (?-1,4 Nsetyl-D-glucosamine polymer). Several types of microorganisms produce chitinase that contributes to basic science and application. The role of chitinase enzyme is widely utilized in various fields, such as health as an anti-tumor agent and agriculture as biological control agents in plant disease caused by fungus. The purpose of this study was to isolate novel chitinolytic bacteria from shrimp shell waste in Makassar, Indonesia, using the scatter plate method on chitin agar selective media incubated at 37°C for 3 days. The clear zone formation around the colony was marked as an indicator of chitinase activity performed by the certain isolate. Selection was carried out by recycling all isolates on chitin agar media, which were incubated at 37°C for 3 days and measured the chitinolytic index. Two of ten isolates produced the highest chitinolytic index, namely, KLA-2 (45) and KLA-4 (15) isolates. The molecular identification results showed that the KLA-2 and KLA-4 isolates were Lysinibacillus fusiformis strain WH22 and Brevibacillus reuszeri strain CMB-15, respectively. Therefore, novel chitinolytic bacteria that can produce chitinase enzyme from shrimp shell waste are L. fusiformis and B. reuszeri
format	article
author	Mashuri Masri EKA SUKMAWATY LASINRANG ADITIA
author_facet	Mashuri Masri EKA SUKMAWATY LASINRANG ADITIA
author_sort	Mashuri Masri
title	Novel chitinolytic bacteria from the shrimp shell processing waste
title_short	Novel chitinolytic bacteria from the shrimp shell processing waste
title_full	Novel chitinolytic bacteria from the shrimp shell processing waste
title_fullStr	Novel chitinolytic bacteria from the shrimp shell processing waste
title_full_unstemmed	Novel chitinolytic bacteria from the shrimp shell processing waste
title_sort	novel chitinolytic bacteria from the shrimp shell processing waste
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/b61348bd94cc4cfdb2e39ea0ca0706ab
work_keys_str_mv	AT mashurimasri novelchitinolyticbacteriafromtheshrimpshellprocessingwaste AT ekasukmawaty novelchitinolyticbacteriafromtheshrimpshellprocessingwaste AT lasinrangaditia novelchitinolyticbacteriafromtheshrimpshellprocessingwaste
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Novel chitinolytic bacteria from the shrimp shell processing waste

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