Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran

Abstract Background The emergence of carbapenem-resistant Pseudomonas aeruginosa is one of the most important challenges in a healthcare setting. The aim of this study is double-locus sequence typing (DLST) typing of bla NDM-1 positive P. aeruginosa isolates. Methods Twenty-nine bla NDM-1 positive i...

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Autores principales:	Mojtaba Shahin, Ali Ahmadi
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Pseudomonas aeruginosa bla NDM-1 DLST MHT MBL Therapeutics. Pharmacology RM1-950 Infectious and parasitic diseases RC109-216 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b613bc3f05ff4d0082d1666945b61dda
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spelling	oai:doaj.org-article:b613bc3f05ff4d0082d1666945b61dda2021-11-07T12:18:09ZMolecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran10.1186/s12941-021-00482-31476-0711https://doaj.org/article/b613bc3f05ff4d0082d1666945b61dda2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12941-021-00482-3https://doaj.org/toc/1476-0711Abstract Background The emergence of carbapenem-resistant Pseudomonas aeruginosa is one of the most important challenges in a healthcare setting. The aim of this study is double-locus sequence typing (DLST) typing of bla NDM-1 positive P. aeruginosa isolates. Methods Twenty-nine bla NDM-1 positive isolates were collected during three years of study from different cities in Iran. Modified hodge test (MHT), double-disk synergy test (DDST) and double-disk potentiation test (DDPT) was performed for detection of carbapenemase and metallo-beta-lactamase (MBL) producing bla NDM-1 positive P. aeruginosa isolates. The antibiotic resistance genes were considered by PCR method. Clonal relationship of bla NDM-1 positive was also characterized using DLST method. Results Antibiotic susceptibility pattern showed that all isolates were resistant to imipenem and ertapenem. DDST and DDPT revealed that 15/29 (51.8%) and 26 (89.7%) of bla NDM-1 positive isolates were MBL producing isolates, respectively. The presence of bla OXA-10, bla VIM-2 , bla IMP-1 and bla SPM genes were detected in 86.2%, 41.4%, 34.5% and 3.5% isolates, respectively. DLST typing results revealed the main cluster were DLST 25-11 with 13 infected or colonized patients. Conclusions The presence of bla NDM-1 gene with other MBLs encoding genes in P. aeruginosa is a potential challenge in the treatment of microorganism infections. DLST showed partial diversity among 29 bla NDM-1 positive isolates.Mojtaba ShahinAli AhmadiBMCarticlePseudomonas aeruginosabla NDM-1DLSTMHTMBLTherapeutics. PharmacologyRM1-950Infectious and parasitic diseasesRC109-216MicrobiologyQR1-502ENAnnals of Clinical Microbiology and Antimicrobials, Vol 20, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas aeruginosa bla NDM-1 DLST MHT MBL Therapeutics. Pharmacology RM1-950 Infectious and parasitic diseases RC109-216 Microbiology QR1-502
spellingShingle	Pseudomonas aeruginosa bla NDM-1 DLST MHT MBL Therapeutics. Pharmacology RM1-950 Infectious and parasitic diseases RC109-216 Microbiology QR1-502 Mojtaba Shahin Ali Ahmadi Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran
description	Abstract Background The emergence of carbapenem-resistant Pseudomonas aeruginosa is one of the most important challenges in a healthcare setting. The aim of this study is double-locus sequence typing (DLST) typing of bla NDM-1 positive P. aeruginosa isolates. Methods Twenty-nine bla NDM-1 positive isolates were collected during three years of study from different cities in Iran. Modified hodge test (MHT), double-disk synergy test (DDST) and double-disk potentiation test (DDPT) was performed for detection of carbapenemase and metallo-beta-lactamase (MBL) producing bla NDM-1 positive P. aeruginosa isolates. The antibiotic resistance genes were considered by PCR method. Clonal relationship of bla NDM-1 positive was also characterized using DLST method. Results Antibiotic susceptibility pattern showed that all isolates were resistant to imipenem and ertapenem. DDST and DDPT revealed that 15/29 (51.8%) and 26 (89.7%) of bla NDM-1 positive isolates were MBL producing isolates, respectively. The presence of bla OXA-10, bla VIM-2 , bla IMP-1 and bla SPM genes were detected in 86.2%, 41.4%, 34.5% and 3.5% isolates, respectively. DLST typing results revealed the main cluster were DLST 25-11 with 13 infected or colonized patients. Conclusions The presence of bla NDM-1 gene with other MBLs encoding genes in P. aeruginosa is a potential challenge in the treatment of microorganism infections. DLST showed partial diversity among 29 bla NDM-1 positive isolates.
format	article
author	Mojtaba Shahin Ali Ahmadi
author_facet	Mojtaba Shahin Ali Ahmadi
author_sort	Mojtaba Shahin
title	Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran
title_short	Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran
title_full	Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran
title_fullStr	Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran
title_full_unstemmed	Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran
title_sort	molecular characterization of ndm-1-producing pseudomonas aeruginosa isolates from hospitalized patients in iran
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b613bc3f05ff4d0082d1666945b61dda
work_keys_str_mv	AT mojtabashahin molecularcharacterizationofndm1producingpseudomonasaeruginosaisolatesfromhospitalizedpatientsiniran AT aliahmadi molecularcharacterizationofndm1producingpseudomonasaeruginosaisolatesfromhospitalizedpatientsiniran
_version_	1718443510789570560

Molecular characterization of NDM-1-producing Pseudomonas aeruginosa isolates from hospitalized patients in Iran

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