Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells

Abstract Ageing is a broad cellular process, largely affecting the immune system, especially T-lymphocytes. Additionally to immunosenescence alone, cytomegalovirus (CMV) infection is thought to have major impacts on T-cell subset composition and exhaustion. These impacts have been studied extensivel...

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Autores principales:	Martine J. Kallemeijn, Anne Mieke H. Boots, Michèle Y. van der Klift, Elisabeth Brouwer, Wayel H. Abdulahad, Jan A. N. Verhaar, Jacques J. M. van Dongen, Anton W. Langerak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6172dab0f5b443a97beab16c2765c3b
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spelling	oai:doaj.org-article:b6172dab0f5b443a97beab16c2765c3b2021-12-02T12:32:12ZAgeing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells10.1038/s41598-017-05849-12045-2322https://doaj.org/article/b6172dab0f5b443a97beab16c2765c3b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05849-1https://doaj.org/toc/2045-2322Abstract Ageing is a broad cellular process, largely affecting the immune system, especially T-lymphocytes. Additionally to immunosenescence alone, cytomegalovirus (CMV) infection is thought to have major impacts on T-cell subset composition and exhaustion. These impacts have been studied extensively in TCRαβ+ T-cells, with reduction in naive, increase in effector (memory) subsets and shifts in CD4/CD8-ratios, in conjunction with morbidity and mortality in elderly. Effects of both ageing and CMV on the TCRγδ+ T-cell compartment remain largely elusive. In the current study we investigated Vγ- and Vδ-usage, maturation, differentiation and exhaustion marker profiles of both CD4 and CD8 double-negative (DN) and CD8+TCRγδ+ T-cells in 157 individuals, age range 20–95. We observed a progressive decrease in absolute numbers of total TCRγδ+ T-cells in blood, affecting the predominant Vγ9/Vδ2 population. Aged TCRγδ+ T-cells appeared to shift from naive to more (late-stage) effector phenotypes, which appeared more prominent in case of persistent CMV infections. In addition, we found effects of both ageing and CMV on the absolute counts of exhausted TCRγδ+ T-cells. Collectively, our data show a clear impact of ageing and CMV persistence on DN and CD8+TCRγδ+ T-cells, similar to what has been reported in CD8+TCRαβ+ T-cells, indicating that they undergo similar ageing processes.Martine J. KallemeijnAnne Mieke H. BootsMichèle Y. van der KliftElisabeth BrouwerWayel H. AbdulahadJan A. N. VerhaarJacques J. M. van DongenAnton W. LangerakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martine J. Kallemeijn Anne Mieke H. Boots Michèle Y. van der Klift Elisabeth Brouwer Wayel H. Abdulahad Jan A. N. Verhaar Jacques J. M. van Dongen Anton W. Langerak Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells
description	Abstract Ageing is a broad cellular process, largely affecting the immune system, especially T-lymphocytes. Additionally to immunosenescence alone, cytomegalovirus (CMV) infection is thought to have major impacts on T-cell subset composition and exhaustion. These impacts have been studied extensively in TCRαβ+ T-cells, with reduction in naive, increase in effector (memory) subsets and shifts in CD4/CD8-ratios, in conjunction with morbidity and mortality in elderly. Effects of both ageing and CMV on the TCRγδ+ T-cell compartment remain largely elusive. In the current study we investigated Vγ- and Vδ-usage, maturation, differentiation and exhaustion marker profiles of both CD4 and CD8 double-negative (DN) and CD8+TCRγδ+ T-cells in 157 individuals, age range 20–95. We observed a progressive decrease in absolute numbers of total TCRγδ+ T-cells in blood, affecting the predominant Vγ9/Vδ2 population. Aged TCRγδ+ T-cells appeared to shift from naive to more (late-stage) effector phenotypes, which appeared more prominent in case of persistent CMV infections. In addition, we found effects of both ageing and CMV on the absolute counts of exhausted TCRγδ+ T-cells. Collectively, our data show a clear impact of ageing and CMV persistence on DN and CD8+TCRγδ+ T-cells, similar to what has been reported in CD8+TCRαβ+ T-cells, indicating that they undergo similar ageing processes.
format	article
author	Martine J. Kallemeijn Anne Mieke H. Boots Michèle Y. van der Klift Elisabeth Brouwer Wayel H. Abdulahad Jan A. N. Verhaar Jacques J. M. van Dongen Anton W. Langerak
author_facet	Martine J. Kallemeijn Anne Mieke H. Boots Michèle Y. van der Klift Elisabeth Brouwer Wayel H. Abdulahad Jan A. N. Verhaar Jacques J. M. van Dongen Anton W. Langerak
author_sort	Martine J. Kallemeijn
title	Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells
title_short	Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells
title_full	Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells
title_fullStr	Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells
title_full_unstemmed	Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells
title_sort	ageing and latent cmv infection impact on maturation, differentiation and exhaustion profiles of t-cell receptor gammadelta t-cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b6172dab0f5b443a97beab16c2765c3b
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_version_	1718394180326129664

Ageing and latent CMV infection impact on maturation, differentiation and exhaustion profiles of T-cell receptor gammadelta T-cells

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