Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis

Long noncoding RNAs (lncRNAs) have been reported to participate in the progression of various cancers, including oral squamous cell carcinoma (OSCC). This study aims to find out whether lncRNA LINC01207 regulates the progression of OSCC. Reverse transcription quantitative polymerase chain reaction (...

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Autores principales:	Xiaolin Lu, Liling Chen, Yang Li, Rong Huang, Xiangfeng Meng, Fangfang Sun
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	oscc autophagy linc01207 mir-1301-3p ldha Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b61c668b38484821b9e813fdfe6797fe
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spelling	oai:doaj.org-article:b61c668b38484821b9e813fdfe6797fe2021-11-17T14:21:59ZLong non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis2165-59792165-598710.1080/21655979.2021.1972784https://doaj.org/article/b61c668b38484821b9e813fdfe6797fe2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1972784https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Long noncoding RNAs (lncRNAs) have been reported to participate in the progression of various cancers, including oral squamous cell carcinoma (OSCC). This study aims to find out whether lncRNA LINC01207 regulates the progression of OSCC. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to evaluate gene expression in OSCC cells and tissues. Cell viability, proliferation, migration, apoptosis, and autophagy were detected using Cell Counting Kit-8 (CCK-8), colony formation, Transwell assays, flow cytometry, and western blot analysis. Luciferase reporter and RNA immunoprecipitation (RIP) assays were conducted to assess the interactions among genes. We found that LINC01207 was overexpressed in OSCC cells and tissues. LINC01207 silencing inhibited OSCC cell proliferation and migration but promoted apoptosis and autophagy, and LINC01207 overexpression had an opposite result. LINC01207 interacted with microRNA-1301-3p (miR-1301-3p) while lactate dehydrogenase isoform A (LHDA) was targeted by miR1301-3p. Effects caused by LINC01207 downregulation on OSCC cells were reversed by overexpression of LDHA. Overall, LINC01207 promotes OSCC progression via the miR-1301-3p/LDHA axisXiaolin LuLiling ChenYang LiRong HuangXiangfeng MengFangfang SunTaylor & Francis Grouparticleosccautophagylinc01207mir-1301-3pldhaBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 7780-7793 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oscc autophagy linc01207 mir-1301-3p ldha Biotechnology TP248.13-248.65
spellingShingle	oscc autophagy linc01207 mir-1301-3p ldha Biotechnology TP248.13-248.65 Xiaolin Lu Liling Chen Yang Li Rong Huang Xiangfeng Meng Fangfang Sun Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis
description	Long noncoding RNAs (lncRNAs) have been reported to participate in the progression of various cancers, including oral squamous cell carcinoma (OSCC). This study aims to find out whether lncRNA LINC01207 regulates the progression of OSCC. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to evaluate gene expression in OSCC cells and tissues. Cell viability, proliferation, migration, apoptosis, and autophagy were detected using Cell Counting Kit-8 (CCK-8), colony formation, Transwell assays, flow cytometry, and western blot analysis. Luciferase reporter and RNA immunoprecipitation (RIP) assays were conducted to assess the interactions among genes. We found that LINC01207 was overexpressed in OSCC cells and tissues. LINC01207 silencing inhibited OSCC cell proliferation and migration but promoted apoptosis and autophagy, and LINC01207 overexpression had an opposite result. LINC01207 interacted with microRNA-1301-3p (miR-1301-3p) while lactate dehydrogenase isoform A (LHDA) was targeted by miR1301-3p. Effects caused by LINC01207 downregulation on OSCC cells were reversed by overexpression of LDHA. Overall, LINC01207 promotes OSCC progression via the miR-1301-3p/LDHA axis
format	article
author	Xiaolin Lu Liling Chen Yang Li Rong Huang Xiangfeng Meng Fangfang Sun
author_facet	Xiaolin Lu Liling Chen Yang Li Rong Huang Xiangfeng Meng Fangfang Sun
author_sort	Xiaolin Lu
title	Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis
title_short	Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis
title_full	Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis
title_fullStr	Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis
title_full_unstemmed	Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis
title_sort	long non-coding rna linc01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microrna-1301-3p/lactate dehydrogenase isoform a axis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/b61c668b38484821b9e813fdfe6797fe
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Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis

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