Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes

Yirong Wang,1 Kewei Fang,2 Shijia He,1 Yang Fan,1 Juming Yu,1 Xiaodong Zhang11Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Sichuan, People’s Republic of China; 2Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Sichuan, People&...

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Autores principales:	Wang Y, Fang K, He S, Fan Y, Yu J, Zhang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	hippocampus iron-containing enzymes neuromechanisms repetitive magnetic stimulation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/b62157cad07a4ab5b0d98df8a13748a7
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spelling	oai:doaj.org-article:b62157cad07a4ab5b0d98df8a13748a72021-12-02T02:21:28ZEffects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes1178-2021https://doaj.org/article/b62157cad07a4ab5b0d98df8a13748a72019-04-01T00:00:00Zhttps://www.dovepress.com/effects-of-repetitive-magnetic-stimulation-on-the-growth-of-primarily--peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yirong Wang,1 Kewei Fang,2 Shijia He,1 Yang Fan,1 Juming Yu,1 Xiaodong Zhang11Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Sichuan, People’s Republic of China; 2Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Sichuan, People’s Republic of ChinaBackground: The mechanism of action of repetitive transcranial magnetic stimulation (rTMS) involves the generation of neuronal and action potentials utilizing induced currents in time-varying magnetic fields. However, the long-lasting and effective biological impact of magnetic stimulation does not appear to be completely explained by the transient magnetic field pulses. In this context, we hypothesized magnetic stimulation may affect the expression of iron-containing enzymes in neurons, mediating the long-lasting biological effects associated with this stimulus.Methods: Primarily cultured hippocampus neurons from SD rats were used as the cell model in this study. These were randomly divided into control, sham, and magnetic stimulation groups to probe into the effect of the magnetic field directly. The latter group received 40%, 60%, and 100% maximal stimulator output Tesla (1.68, 2.52, and 4.2 T) with low-frequency rTMS (1 Hz). The expression of iron-containing enzymes (catalase and aconitase) and non-ferrous enzymes (protein kinase A) was measured with Western blotting and ELISA.Results: The survival rates of neurons in the 40%T and 60%T groups were significantly increased in comparison to the controls (P<0.05), while those in the 100%T group showed cell damage, with slightly disturbed neurite connections and decreased survival rate. Furthermore, catalase and aconitase expression was higher in all of the stimulated groups in comparison to controls (P<0.05). On the other hand, the expression of the iron-containing enzymes decreased in the 100%T group in comparison with the 40%T and 60%T groups (P<0.05). Meanwhile, the expression of protein kinase A was not significantly increased in the groups which underwent magnetic stimulation.Conclusion: rTMS may increase the expression of ferrous enzymes but does not have a strong effect on non-ferrous enzymes. Excessive intensity of magnetic stimulation may reduce neuronal survival rate and affect the expression of iron-containing enzymes. The mechanism underlying the lasting effect of rTMS may be related to the increase of ferriferous expression induced by magnetic stimulation, with a clear correlation with stimulation intensity.Keywords: hippocampus, iron-containing enzymes, neuromechanisms, repetitive magnetic stimulationWang YFang KHe SFan YYu JZhang XDove Medical Pressarticlehippocampusiron-containing enzymesneuromechanismsrepetitive magnetic stimulationNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 927-934 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	hippocampus iron-containing enzymes neuromechanisms repetitive magnetic stimulation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	hippocampus iron-containing enzymes neuromechanisms repetitive magnetic stimulation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Wang Y Fang K He S Fan Y Yu J Zhang X Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes
description	Yirong Wang,1 Kewei Fang,2 Shijia He,1 Yang Fan,1 Juming Yu,1 Xiaodong Zhang11Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Sichuan, People’s Republic of China; 2Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Sichuan, People’s Republic of ChinaBackground: The mechanism of action of repetitive transcranial magnetic stimulation (rTMS) involves the generation of neuronal and action potentials utilizing induced currents in time-varying magnetic fields. However, the long-lasting and effective biological impact of magnetic stimulation does not appear to be completely explained by the transient magnetic field pulses. In this context, we hypothesized magnetic stimulation may affect the expression of iron-containing enzymes in neurons, mediating the long-lasting biological effects associated with this stimulus.Methods: Primarily cultured hippocampus neurons from SD rats were used as the cell model in this study. These were randomly divided into control, sham, and magnetic stimulation groups to probe into the effect of the magnetic field directly. The latter group received 40%, 60%, and 100% maximal stimulator output Tesla (1.68, 2.52, and 4.2 T) with low-frequency rTMS (1 Hz). The expression of iron-containing enzymes (catalase and aconitase) and non-ferrous enzymes (protein kinase A) was measured with Western blotting and ELISA.Results: The survival rates of neurons in the 40%T and 60%T groups were significantly increased in comparison to the controls (P<0.05), while those in the 100%T group showed cell damage, with slightly disturbed neurite connections and decreased survival rate. Furthermore, catalase and aconitase expression was higher in all of the stimulated groups in comparison to controls (P<0.05). On the other hand, the expression of the iron-containing enzymes decreased in the 100%T group in comparison with the 40%T and 60%T groups (P<0.05). Meanwhile, the expression of protein kinase A was not significantly increased in the groups which underwent magnetic stimulation.Conclusion: rTMS may increase the expression of ferrous enzymes but does not have a strong effect on non-ferrous enzymes. Excessive intensity of magnetic stimulation may reduce neuronal survival rate and affect the expression of iron-containing enzymes. The mechanism underlying the lasting effect of rTMS may be related to the increase of ferriferous expression induced by magnetic stimulation, with a clear correlation with stimulation intensity.Keywords: hippocampus, iron-containing enzymes, neuromechanisms, repetitive magnetic stimulation
format	article
author	Wang Y Fang K He S Fan Y Yu J Zhang X
author_facet	Wang Y Fang K He S Fan Y Yu J Zhang X
author_sort	Wang Y
title	Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes
title_short	Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes
title_full	Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes
title_fullStr	Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes
title_full_unstemmed	Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes
title_sort	effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/b62157cad07a4ab5b0d98df8a13748a7
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Effects of repetitive magnetic stimulation on the growth of primarily cultured hippocampus neurons in vitro and their expression of iron-containing enzymes

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