Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections

Single-cell RNA sequencing (scRNA-seq) offers the possibility to monitor both host and pathogens transcriptomes at the cellular level. Here, public scRNA-seq datasets from <i>Drosophila melanogaster</i> midgut cells were used to compare the differences in replication strategy and cellula...

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Autores principales:	João M. F. Silva, Tatsuya Nagata, Fernando L. Melo, Santiago F. Elena
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell-type-specific gene expression <i>Drosophila</i> viruses dual RNA-seq single-cell genomics single-cell RNA-seq virus-host interaction Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b62b6eb8ea2c434d858ffbbce73d131b
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spelling	oai:doaj.org-article:b62b6eb8ea2c434d858ffbbce73d131b2021-11-25T19:14:09ZHeterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections10.3390/v131122841999-4915https://doaj.org/article/b62b6eb8ea2c434d858ffbbce73d131b2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2284https://doaj.org/toc/1999-4915Single-cell RNA sequencing (scRNA-seq) offers the possibility to monitor both host and pathogens transcriptomes at the cellular level. Here, public scRNA-seq datasets from <i>Drosophila melanogaster</i> midgut cells were used to compare the differences in replication strategy and cellular responses between two fly picorna-like viruses, Thika virus (TV) and <i>D. melanogaster</i> Nora virus (DMelNV). TV exhibited lower levels of viral RNA accumulation but infected a higher number of cells compared to DMelNV. In both cases, viral RNA accumulation varied according to cell subtype. The cellular heat shock response to TV and DMelNV infection was cell-subtype- and virus-specific. Disruption of bottleneck genes at later stages of infection in the systemic response, as well as of translation-related genes in the cellular response to DMelNV in two cell subtypes, may affect the virus replication.João M. F. SilvaTatsuya NagataFernando L. MeloSantiago F. ElenaMDPI AGarticlecell-type-specific gene expression<i>Drosophila</i> virusesdual RNA-seqsingle-cell genomicssingle-cell RNA-seqvirus-host interactionMicrobiologyQR1-502ENViruses, Vol 13, Iss 2284, p 2284 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell-type-specific gene expression <i>Drosophila</i> viruses dual RNA-seq single-cell genomics single-cell RNA-seq virus-host interaction Microbiology QR1-502
spellingShingle	cell-type-specific gene expression <i>Drosophila</i> viruses dual RNA-seq single-cell genomics single-cell RNA-seq virus-host interaction Microbiology QR1-502 João M. F. Silva Tatsuya Nagata Fernando L. Melo Santiago F. Elena Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections
description	Single-cell RNA sequencing (scRNA-seq) offers the possibility to monitor both host and pathogens transcriptomes at the cellular level. Here, public scRNA-seq datasets from <i>Drosophila melanogaster</i> midgut cells were used to compare the differences in replication strategy and cellular responses between two fly picorna-like viruses, Thika virus (TV) and <i>D. melanogaster</i> Nora virus (DMelNV). TV exhibited lower levels of viral RNA accumulation but infected a higher number of cells compared to DMelNV. In both cases, viral RNA accumulation varied according to cell subtype. The cellular heat shock response to TV and DMelNV infection was cell-subtype- and virus-specific. Disruption of bottleneck genes at later stages of infection in the systemic response, as well as of translation-related genes in the cellular response to DMelNV in two cell subtypes, may affect the virus replication.
format	article
author	João M. F. Silva Tatsuya Nagata Fernando L. Melo Santiago F. Elena
author_facet	João M. F. Silva Tatsuya Nagata Fernando L. Melo Santiago F. Elena
author_sort	João M. F. Silva
title	Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections
title_short	Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections
title_full	Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections
title_fullStr	Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections
title_full_unstemmed	Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections
title_sort	heterogeneity in the response of different subtypes of <i>drosophila melanogaster</i> midgut cells to viral infections
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b62b6eb8ea2c434d858ffbbce73d131b
work_keys_str_mv	AT joaomfsilva heterogeneityintheresponseofdifferentsubtypesofidrosophilamelanogasterimidgutcellstoviralinfections AT tatsuyanagata heterogeneityintheresponseofdifferentsubtypesofidrosophilamelanogasterimidgutcellstoviralinfections AT fernandolmelo heterogeneityintheresponseofdifferentsubtypesofidrosophilamelanogasterimidgutcellstoviralinfections AT santiagofelena heterogeneityintheresponseofdifferentsubtypesofidrosophilamelanogasterimidgutcellstoviralinfections
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Heterogeneity in the Response of Different Subtypes of <i>Drosophila melanogaster</i> Midgut Cells to Viral Infections

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