Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer

Long Xia Chen,* Xiao Ling Ni,* Heng Zhang, Min Wu, Jing Liu, Shan Xu, Ling Lin Yang, Shao Zhi Fu, Jingbo Wu Department of Oncology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of China *These authors contributed equally to this work Introduction: T...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen L, Ni X, Zhang H, Wu M, Liu J, Xu S, Yang L, Fu S, Wu J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Thalidomide (THA) Nanoparticles MPEG-PCL Lung cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b62ed503a54848e1936267b41c88feee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b62ed503a54848e1936267b41c88feee
record_format	dspace
spelling	oai:doaj.org-article:b62ed503a54848e1936267b41c88feee2021-12-02T00:02:52ZPreparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer1178-2013https://doaj.org/article/b62ed503a54848e1936267b41c88feee2018-04-01T00:00:00Zhttps://www.dovepress.com/preparation-characterization-in-vitro-and-in-vivo-anti-tumor-effect-of-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Long Xia Chen,* Xiao Ling Ni,* Heng Zhang, Min Wu, Jing Liu, Shan Xu, Ling Lin Yang, Shao Zhi Fu, Jingbo Wu Department of Oncology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of China *These authors contributed equally to this work Introduction: Thalidomide (THA) is an angiogenesis inhibitor and an efficient inhibitor of the tumor necrosis factor-α (TNF-α). However, the clinical application of THA has been limited due to hydrophobicity of the compound. Materials and methods: To increase the water solubility of THA and in order to evaluate the anticancer abilities of this material on human lung carcinoma, methoxy poly(ethylene glycol)-poly(ε-caprolactone) nanoparticles loaded with THA (THA-NPs) were prepared. The synthesis of THA-NPs was carried out via a dialysis method with relative satisfactory encapsulation efficiency, loading capacity, size distribution, and zeta potential. Results: A cytotoxicity assay demonstrated that THA-NPs inhibited the growth of cells in a dose-dependent manner. The evaluation of anti-tumor activity in vivo showed that THA-NPs could inhibit tumor growth and prolong the survival rate of tumor-bearing mice. Immunohistochemical analysis indicated that THA-NPs inhibited cell proliferation (Ki-67 positive rate, 32.8%±4.2%, P<0.01), and resulted in a decreased rate of the tumor tissue microvessel density (3.87%±0.77%, P<0.01), VEGF (26.67%±4.02%, P<0.01), and TNF-α (75.21±6.85 ng/mL, P<0.01). Conclusion: In general, the drug delivery system reported herein may shed light on future targeted therapy in lung cancer treatment. Keywords: thalidomide, nanoparticles, MPEG-PCL, lung cancerChen LNi XZhang HWu MLiu JXu SYang LFu SWu JDove Medical PressarticleThalidomide (THA)NanoparticlesMPEG-PCLLung cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2463-2476 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Thalidomide (THA) Nanoparticles MPEG-PCL Lung cancer Medicine (General) R5-920
spellingShingle	Thalidomide (THA) Nanoparticles MPEG-PCL Lung cancer Medicine (General) R5-920 Chen L Ni X Zhang H Wu M Liu J Xu S Yang L Fu S Wu J Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
description	Long Xia Chen,* Xiao Ling Ni,* Heng Zhang, Min Wu, Jing Liu, Shan Xu, Ling Lin Yang, Shao Zhi Fu, Jingbo Wu Department of Oncology, the Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of China *These authors contributed equally to this work Introduction: Thalidomide (THA) is an angiogenesis inhibitor and an efficient inhibitor of the tumor necrosis factor-α (TNF-α). However, the clinical application of THA has been limited due to hydrophobicity of the compound. Materials and methods: To increase the water solubility of THA and in order to evaluate the anticancer abilities of this material on human lung carcinoma, methoxy poly(ethylene glycol)-poly(ε-caprolactone) nanoparticles loaded with THA (THA-NPs) were prepared. The synthesis of THA-NPs was carried out via a dialysis method with relative satisfactory encapsulation efficiency, loading capacity, size distribution, and zeta potential. Results: A cytotoxicity assay demonstrated that THA-NPs inhibited the growth of cells in a dose-dependent manner. The evaluation of anti-tumor activity in vivo showed that THA-NPs could inhibit tumor growth and prolong the survival rate of tumor-bearing mice. Immunohistochemical analysis indicated that THA-NPs inhibited cell proliferation (Ki-67 positive rate, 32.8%±4.2%, P<0.01), and resulted in a decreased rate of the tumor tissue microvessel density (3.87%±0.77%, P<0.01), VEGF (26.67%±4.02%, P<0.01), and TNF-α (75.21±6.85 ng/mL, P<0.01). Conclusion: In general, the drug delivery system reported herein may shed light on future targeted therapy in lung cancer treatment. Keywords: thalidomide, nanoparticles, MPEG-PCL, lung cancer
format	article
author	Chen L Ni X Zhang H Wu M Liu J Xu S Yang L Fu S Wu J
author_facet	Chen L Ni X Zhang H Wu M Liu J Xu S Yang L Fu S Wu J
author_sort	Chen L
title	Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
title_short	Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
title_full	Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
title_fullStr	Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
title_full_unstemmed	Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
title_sort	preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/b62ed503a54848e1936267b41c88feee
work_keys_str_mv	AT chenl preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT nix preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT zhangh preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT wum preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT liuj preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT xus preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT yangl preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT fus preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer AT wuj preparationcharacterizationinvitroandinvivoantitumoreffectofthalidomidenanoparticlesonlungcancer
_version_	1718404009469935616

Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer

Ejemplares similares