Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

Golden pompano (Trachinotus ovatus) is one of the most economically critical marine fish in South China. Low oxygen stress has resulted in substantial economic losses to the aquaculture of T. ovatus. However, the molecular responses of fish gills to hypoxia challenge remain unclear. To understand th...

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Autores principales:	Lize San, Baosuo Liu, Bo Liu, Huayang Guo, Liang Guo, Nan Zhang, Kecheng Zhu, Shigui Jiang, Dianchang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Trachinotus ovatus hypoxia stress transcriptome gene expression gills Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/b637b74a0a1a4d7d8abd24d23a302cae
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spelling	oai:doaj.org-article:b637b74a0a1a4d7d8abd24d23a302cae2021-12-02T11:07:01ZTranscriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)2296-774510.3389/fmars.2021.763622https://doaj.org/article/b637b74a0a1a4d7d8abd24d23a302cae2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.763622/fullhttps://doaj.org/toc/2296-7745Golden pompano (Trachinotus ovatus) is one of the most economically critical marine fish in South China. Low oxygen stress has resulted in substantial economic losses to the aquaculture of T. ovatus. However, the molecular responses of fish gills to hypoxia challenge remain unclear. To understand the mechanism underlying adaption to hypoxia, we analyzed the transcriptome of T. ovatus gills in response to hypoxic stress in the normal oxygen group, hypoxic group, and hypoxia treatment after oxygen recovery group. This study found that hypoxia for 8 h was the critical time of hypoxic stress and corresponded to the largest number of differentially expressed genes. After hypoxic stress, genes for chemokines, chemokine receptors, interleukins, complement factors, and other cytokines were significantly downregulated, which may be why fish are vulnerable to pathogen infection in a hypoxic environment. According to a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, many downregulated genes were significantly enriched in the steroid biosynthesis, focal adhesion, and the extracellular matrix (ECM)-receptor interaction signal pathways, which affected cell signal transduction, adhesion, and apoptosis. Compared with the hypoxic group, the amounts of upregulated genes related to phagocytosis and protein degradation were upregulated in the dissolved oxygen recovery group. These results indicated that after the recovery of dissolved oxygen, the fish body repaired the stress-induced damage by rapidly removing misfolded proteins. These findings provide a better understanding of the hypoxia response mechanism of fish and represent a useful resource for the genetic breeding of T. ovatus.Lize SanLize SanBaosuo LiuBaosuo LiuBaosuo LiuBo LiuBo LiuHuayang GuoHuayang GuoHuayang GuoLiang GuoLiang GuoNan ZhangNan ZhangKecheng ZhuKecheng ZhuKecheng ZhuKecheng ZhuShigui JiangShigui JiangDianchang ZhangDianchang ZhangDianchang ZhangDianchang ZhangFrontiers Media S.A.articleTrachinotus ovatushypoxia stresstranscriptomegene expressiongillsScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trachinotus ovatus hypoxia stress transcriptome gene expression gills Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	Trachinotus ovatus hypoxia stress transcriptome gene expression gills Science Q General. Including nature conservation, geographical distribution QH1-199.5 Lize San Lize San Baosuo Liu Baosuo Liu Baosuo Liu Bo Liu Bo Liu Huayang Guo Huayang Guo Huayang Guo Liang Guo Liang Guo Nan Zhang Nan Zhang Kecheng Zhu Kecheng Zhu Kecheng Zhu Kecheng Zhu Shigui Jiang Shigui Jiang Dianchang Zhang Dianchang Zhang Dianchang Zhang Dianchang Zhang Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
description	Golden pompano (Trachinotus ovatus) is one of the most economically critical marine fish in South China. Low oxygen stress has resulted in substantial economic losses to the aquaculture of T. ovatus. However, the molecular responses of fish gills to hypoxia challenge remain unclear. To understand the mechanism underlying adaption to hypoxia, we analyzed the transcriptome of T. ovatus gills in response to hypoxic stress in the normal oxygen group, hypoxic group, and hypoxia treatment after oxygen recovery group. This study found that hypoxia for 8 h was the critical time of hypoxic stress and corresponded to the largest number of differentially expressed genes. After hypoxic stress, genes for chemokines, chemokine receptors, interleukins, complement factors, and other cytokines were significantly downregulated, which may be why fish are vulnerable to pathogen infection in a hypoxic environment. According to a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, many downregulated genes were significantly enriched in the steroid biosynthesis, focal adhesion, and the extracellular matrix (ECM)-receptor interaction signal pathways, which affected cell signal transduction, adhesion, and apoptosis. Compared with the hypoxic group, the amounts of upregulated genes related to phagocytosis and protein degradation were upregulated in the dissolved oxygen recovery group. These results indicated that after the recovery of dissolved oxygen, the fish body repaired the stress-induced damage by rapidly removing misfolded proteins. These findings provide a better understanding of the hypoxia response mechanism of fish and represent a useful resource for the genetic breeding of T. ovatus.
format	article
author	Lize San Lize San Baosuo Liu Baosuo Liu Baosuo Liu Bo Liu Bo Liu Huayang Guo Huayang Guo Huayang Guo Liang Guo Liang Guo Nan Zhang Nan Zhang Kecheng Zhu Kecheng Zhu Kecheng Zhu Kecheng Zhu Shigui Jiang Shigui Jiang Dianchang Zhang Dianchang Zhang Dianchang Zhang Dianchang Zhang
author_facet	Lize San Lize San Baosuo Liu Baosuo Liu Baosuo Liu Bo Liu Bo Liu Huayang Guo Huayang Guo Huayang Guo Liang Guo Liang Guo Nan Zhang Nan Zhang Kecheng Zhu Kecheng Zhu Kecheng Zhu Kecheng Zhu Shigui Jiang Shigui Jiang Dianchang Zhang Dianchang Zhang Dianchang Zhang Dianchang Zhang
author_sort	Lize San
title	Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
title_short	Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
title_full	Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
title_fullStr	Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
title_full_unstemmed	Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)
title_sort	transcriptome analysis of gills provides insights into translation changes under hypoxic stress and reoxygenation in golden pompano, trachinotus ovatus (linnaeus 1758)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b637b74a0a1a4d7d8abd24d23a302cae
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Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

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