Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.

Dendritic cells (DCs) play a major role in the innate immune response since they recognize a broad repertoire of PAMPs mainly via Toll-like receptors (TLRs). During Helicobacter pylori (H. pylori) infection, TLRs have been shown to be important to control cytokine response particularly in murine DCs...

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Autores principales:	Romy Käbisch, Raquel Mejías-Luque, Markus Gerhard, Christian Prinz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b63ddad6ab6d44f3931c84728f5cea6c
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spelling	oai:doaj.org-article:b63ddad6ab6d44f3931c84728f5cea6c2021-11-25T06:03:19ZInvolvement of Toll-like receptors on Helicobacter pylori-induced immunity.1932-620310.1371/journal.pone.0104804https://doaj.org/article/b63ddad6ab6d44f3931c84728f5cea6c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25153703/?tool=EBIhttps://doaj.org/toc/1932-6203Dendritic cells (DCs) play a major role in the innate immune response since they recognize a broad repertoire of PAMPs mainly via Toll-like receptors (TLRs). During Helicobacter pylori (H. pylori) infection, TLRs have been shown to be important to control cytokine response particularly in murine DCs. In the present study we analyzed the effect of blocking TLRs on human DCs. Co-incubation of human DCs with H. pylori resulted in the release of the pro-inflammatory cytokines IL-12p70, IL-6 and IL-10. Release of IL-12p70 and IL-10 was predominantly influenced when TLR4 signaling was blocked by adding specific antibodies, suggesting a strong influence on subsequent T cell responses through TLR4 activation on DCs. Co-incubation of H. pylori-primed DC with allogeneic CD4+ T cells resulted in the production of IFN-γ and IL-17A as well as the expression of Foxp3, validating a mixed Th1/Th17 and Treg response in vitro. Neutralization of TLR4 during H. pylori infection resulted in significantly decreased amounts of IL-17A and IFN-γ and reduced levels of Foxp3-expressing and IL-10-secreting T cells. Our findings suggest that DC cytokine secretion induced upon TLR4-mediated recognition of H. pylori influences inflammatory and regulatory T cell responses, which might facilitate the chronic bacterial persistence.Romy KäbischRaquel Mejías-LuqueMarkus GerhardChristian PrinzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104804 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Romy Käbisch Raquel Mejías-Luque Markus Gerhard Christian Prinz Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
description	Dendritic cells (DCs) play a major role in the innate immune response since they recognize a broad repertoire of PAMPs mainly via Toll-like receptors (TLRs). During Helicobacter pylori (H. pylori) infection, TLRs have been shown to be important to control cytokine response particularly in murine DCs. In the present study we analyzed the effect of blocking TLRs on human DCs. Co-incubation of human DCs with H. pylori resulted in the release of the pro-inflammatory cytokines IL-12p70, IL-6 and IL-10. Release of IL-12p70 and IL-10 was predominantly influenced when TLR4 signaling was blocked by adding specific antibodies, suggesting a strong influence on subsequent T cell responses through TLR4 activation on DCs. Co-incubation of H. pylori-primed DC with allogeneic CD4+ T cells resulted in the production of IFN-γ and IL-17A as well as the expression of Foxp3, validating a mixed Th1/Th17 and Treg response in vitro. Neutralization of TLR4 during H. pylori infection resulted in significantly decreased amounts of IL-17A and IFN-γ and reduced levels of Foxp3-expressing and IL-10-secreting T cells. Our findings suggest that DC cytokine secretion induced upon TLR4-mediated recognition of H. pylori influences inflammatory and regulatory T cell responses, which might facilitate the chronic bacterial persistence.
format	article
author	Romy Käbisch Raquel Mejías-Luque Markus Gerhard Christian Prinz
author_facet	Romy Käbisch Raquel Mejías-Luque Markus Gerhard Christian Prinz
author_sort	Romy Käbisch
title	Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
title_short	Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
title_full	Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
title_fullStr	Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
title_full_unstemmed	Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
title_sort	involvement of toll-like receptors on helicobacter pylori-induced immunity.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b63ddad6ab6d44f3931c84728f5cea6c
work_keys_str_mv	AT romykabisch involvementoftolllikereceptorsonhelicobacterpyloriinducedimmunity AT raquelmejiasluque involvementoftolllikereceptorsonhelicobacterpyloriinducedimmunity AT markusgerhard involvementoftolllikereceptorsonhelicobacterpyloriinducedimmunity AT christianprinz involvementoftolllikereceptorsonhelicobacterpyloriinducedimmunity
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Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.

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