Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

Summary: Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes h...

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Autores principales:	H. Erdinc Besikcioglu, Ümmügülsüm Yurteri, Enkhtsetseg Munkhbaatar, Linhan Ye, Fangfang Zhang, Alessandra Moretti, Carmen Mota Reyes, Candan Özoğul, Helmut Friess, Güralp O. Ceyhan, Rouzanna Istvanffy, Ihsan Ekin Demir
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell culture Cell isolation Cancer Model Organisms Neuroscience Stem Cells Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/b642699e60d54a329d6538dd1f56fe16
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spelling	oai:doaj.org-article:b642699e60d54a329d6538dd1f56fe162021-11-20T05:13:50ZInnervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer2666-166710.1016/j.xpro.2021.100935https://doaj.org/article/b642699e60d54a329d6538dd1f56fe162021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006419https://doaj.org/toc/2666-1667Summary: Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras+/LSLG12D Trp53fl/f lp48+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons.For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).H. Erdinc BesikciogluÜmmügülsüm YurteriEnkhtsetseg MunkhbaatarLinhan YeFangfang ZhangAlessandra MorettiCarmen Mota ReyesCandan ÖzoğulHelmut FriessGüralp O. CeyhanRouzanna IstvanffyIhsan Ekin DemirElsevierarticleCell cultureCell isolationCancerModel OrganismsNeuroscienceStem CellsScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100935- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell culture Cell isolation Cancer Model Organisms Neuroscience Stem Cells Science (General) Q1-390
spellingShingle	Cell culture Cell isolation Cancer Model Organisms Neuroscience Stem Cells Science (General) Q1-390 H. Erdinc Besikcioglu Ümmügülsüm Yurteri Enkhtsetseg Munkhbaatar Linhan Ye Fangfang Zhang Alessandra Moretti Carmen Mota Reyes Candan Özoğul Helmut Friess Güralp O. Ceyhan Rouzanna Istvanffy Ihsan Ekin Demir Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer
description	Summary: Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras+/LSLG12D Trp53fl/f lp48+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons.For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).
format	article
author	H. Erdinc Besikcioglu Ümmügülsüm Yurteri Enkhtsetseg Munkhbaatar Linhan Ye Fangfang Zhang Alessandra Moretti Carmen Mota Reyes Candan Özoğul Helmut Friess Güralp O. Ceyhan Rouzanna Istvanffy Ihsan Ekin Demir
author_facet	H. Erdinc Besikcioglu Ümmügülsüm Yurteri Enkhtsetseg Munkhbaatar Linhan Ye Fangfang Zhang Alessandra Moretti Carmen Mota Reyes Candan Özoğul Helmut Friess Güralp O. Ceyhan Rouzanna Istvanffy Ihsan Ekin Demir
author_sort	H. Erdinc Besikcioglu
title	Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer
title_short	Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer
title_full	Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer
title_fullStr	Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer
title_full_unstemmed	Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer
title_sort	innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/b642699e60d54a329d6538dd1f56fe16
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Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

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