Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency

Namrata Sharma,1 Sujata Mohanty,2 Vishal Jhanji,3,4 Rasik B Vajpayee4–6 1Cornea, Cataract and Refractive Surgery Services, Dr Rajendra Prasad Center for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India; 2Stem Cell Facility DBT – Centre of Excellence...

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Autores principales:	Sharma N, Mohanty S, Jhanji V, Vajpayee RB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	partial LSCD amniotic membrane transplantation Limbal stem cell transplantation Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/b64eee9a77f04c3ca46a22595e949275
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spelling	oai:doaj.org-article:b64eee9a77f04c3ca46a22595e9492752021-12-02T11:21:06ZAmniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency1177-5483https://doaj.org/article/b64eee9a77f04c3ca46a22595e9492752018-10-01T00:00:00Zhttps://www.dovepress.com/amniotic-membrane-transplantation-with-or-without-autologous-cultivate-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Namrata Sharma,1 Sujata Mohanty,2 Vishal Jhanji,3,4 Rasik B Vajpayee4–6 1Cornea, Cataract and Refractive Surgery Services, Dr Rajendra Prasad Center for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India; 2Stem Cell Facility DBT – Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, India; 3Cornea, Cataract and External Disease Services, Refractive Surgery Service, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; 4Centre for Eye Research Australia, University of Melbourne, Melbourne, Australia; 5Royal Victorian Eye and Ear Hospital, Melbourne, Australia; 6Cornea, Cataract & Refractive Surgery Services, Vision Eye Institute, Melbourne, Australia Purpose: To compare the outcomes of amniotic membrane transplantation (AMT) vs cultivated limbal stem cell transplantation (LSCT) in eyes with partial limbal stem cell deficiency (LSCD) following chemical burns.Methods: Eyes with unilateral partial LSCD (≤180° involvement) were randomized in two groups to undergo either pannus resection combined with AMT or pannus resection combined with LSCT in a tertiary eye care hospital. Primary outcome measures were time to corneal epithelialization and absence of conjunctivalization of the cornea. Patients were followed up at 1 week, 1, 3, 6, and 12 months after the surgical procedure.Results: There was no difference between mean age (30.85±5.8 vs 28.64±6.4 years, P=0.40) and sex distribution of patients between the two groups at baseline. Mean time to corneal epithelialization was 10.45±5.8 days in the AMT group and 11±3.9 days in the LSCT group (P=0.43). At the end of 1 year, there was no significant difference between the degree of conjunctivalization of cornea, (P=0.06) corneal vascularization, (P=0.08), and clarity (P=0.07) in both groups.Conclusion: Our study showed that AMT alone is a useful therapeutic modality in cases with partial LSCD due to ocular chemical injury. Stem cell transplantation may not be required in these cases. Keywords: partial LSCD, amniotic membrane transplantation, limbal stem cell transplantationSharma NMohanty SJhanji VVajpayee RBDove Medical Pressarticlepartial LSCDamniotic membrane transplantationLimbal stem cell transplantationOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 2103-2106 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	partial LSCD amniotic membrane transplantation Limbal stem cell transplantation Ophthalmology RE1-994
spellingShingle	partial LSCD amniotic membrane transplantation Limbal stem cell transplantation Ophthalmology RE1-994 Sharma N Mohanty S Jhanji V Vajpayee RB Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency
description	Namrata Sharma,1 Sujata Mohanty,2 Vishal Jhanji,3,4 Rasik B Vajpayee4–6 1Cornea, Cataract and Refractive Surgery Services, Dr Rajendra Prasad Center for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India; 2Stem Cell Facility DBT – Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, India; 3Cornea, Cataract and External Disease Services, Refractive Surgery Service, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; 4Centre for Eye Research Australia, University of Melbourne, Melbourne, Australia; 5Royal Victorian Eye and Ear Hospital, Melbourne, Australia; 6Cornea, Cataract & Refractive Surgery Services, Vision Eye Institute, Melbourne, Australia Purpose: To compare the outcomes of amniotic membrane transplantation (AMT) vs cultivated limbal stem cell transplantation (LSCT) in eyes with partial limbal stem cell deficiency (LSCD) following chemical burns.Methods: Eyes with unilateral partial LSCD (≤180° involvement) were randomized in two groups to undergo either pannus resection combined with AMT or pannus resection combined with LSCT in a tertiary eye care hospital. Primary outcome measures were time to corneal epithelialization and absence of conjunctivalization of the cornea. Patients were followed up at 1 week, 1, 3, 6, and 12 months after the surgical procedure.Results: There was no difference between mean age (30.85±5.8 vs 28.64±6.4 years, P=0.40) and sex distribution of patients between the two groups at baseline. Mean time to corneal epithelialization was 10.45±5.8 days in the AMT group and 11±3.9 days in the LSCT group (P=0.43). At the end of 1 year, there was no significant difference between the degree of conjunctivalization of cornea, (P=0.06) corneal vascularization, (P=0.08), and clarity (P=0.07) in both groups.Conclusion: Our study showed that AMT alone is a useful therapeutic modality in cases with partial LSCD due to ocular chemical injury. Stem cell transplantation may not be required in these cases. Keywords: partial LSCD, amniotic membrane transplantation, limbal stem cell transplantation
format	article
author	Sharma N Mohanty S Jhanji V Vajpayee RB
author_facet	Sharma N Mohanty S Jhanji V Vajpayee RB
author_sort	Sharma N
title	Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency
title_short	Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency
title_full	Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency
title_fullStr	Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency
title_full_unstemmed	Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency
title_sort	amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/b64eee9a77f04c3ca46a22595e949275
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Amniotic membrane transplantation with or without autologous cultivated limbal stem cell transplantation for the management of partial limbal stem cell deficiency

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