Characterization of a novel lysozyme-like 4 gene in the rat.

Lysozyme-like proteins (LYZLs) belong to the class of c-type lysozymes and are not well characterized in many species including the rat. In this study, using in silico and molecular biology techniques, we report the identification, cloning and characterization of rat Lyzl4 gene and also determine th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ganapathy Narmadha, Katakam Muneswararao, Angireddy Rajesh, Suresh Yenugu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b64fde752b3e47908f2355f06955e6b9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b64fde752b3e47908f2355f06955e6b9
record_format	dspace
spelling	oai:doaj.org-article:b64fde752b3e47908f2355f06955e6b92021-11-18T07:34:17ZCharacterization of a novel lysozyme-like 4 gene in the rat.1932-620310.1371/journal.pone.0027659https://doaj.org/article/b64fde752b3e47908f2355f06955e6b92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22110709/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Lysozyme-like proteins (LYZLs) belong to the class of c-type lysozymes and are not well characterized in many species including the rat. In this study, using in silico and molecular biology techniques, we report the identification, cloning and characterization of rat Lyzl4 gene and also determine the expression pattern of Lyzl1, Lyzl3 and Lyzl6. The rat Lyzl genes were found to be distributed on three chromosomes and all of them retained the characteristic eight cysteine signature of c-type lysozyme. Homology modeling of rat LYZL4 indicated that its structure is similar to that of the mouse SLLP1. In the male reproductive tract of rat, Lyzl gene expression was confined to the testis. Lyzl1 and Lyzl4 were found to be expressed in tissues beyond the male reproductive tract, whereas Lyzl3 and Lyzl6 were not. Lyzl expression in the developing (10-60 day old) rats was androgen dependent in the testis. Immunodetection using antibodies against rat LYZL4 revealed the presence of LYZL4 protein in the germinal layer of the testes and on the sperm tail. Recombinant LYZL4 did not exhibit antibacterial, muramidase and isopeptidase activities characteristic to c-type lysozyme. To the best of our knowledge, for the first time we report the characterization of Lyzl genes in the rat. Results of our study indicate that rat LYZL proteins may have an important role in male reproductive tract function.Ganapathy NarmadhaKatakam MuneswararaoAngireddy RajeshSuresh YenuguPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27659 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ganapathy Narmadha Katakam Muneswararao Angireddy Rajesh Suresh Yenugu Characterization of a novel lysozyme-like 4 gene in the rat.
description	Lysozyme-like proteins (LYZLs) belong to the class of c-type lysozymes and are not well characterized in many species including the rat. In this study, using in silico and molecular biology techniques, we report the identification, cloning and characterization of rat Lyzl4 gene and also determine the expression pattern of Lyzl1, Lyzl3 and Lyzl6. The rat Lyzl genes were found to be distributed on three chromosomes and all of them retained the characteristic eight cysteine signature of c-type lysozyme. Homology modeling of rat LYZL4 indicated that its structure is similar to that of the mouse SLLP1. In the male reproductive tract of rat, Lyzl gene expression was confined to the testis. Lyzl1 and Lyzl4 were found to be expressed in tissues beyond the male reproductive tract, whereas Lyzl3 and Lyzl6 were not. Lyzl expression in the developing (10-60 day old) rats was androgen dependent in the testis. Immunodetection using antibodies against rat LYZL4 revealed the presence of LYZL4 protein in the germinal layer of the testes and on the sperm tail. Recombinant LYZL4 did not exhibit antibacterial, muramidase and isopeptidase activities characteristic to c-type lysozyme. To the best of our knowledge, for the first time we report the characterization of Lyzl genes in the rat. Results of our study indicate that rat LYZL proteins may have an important role in male reproductive tract function.
format	article
author	Ganapathy Narmadha Katakam Muneswararao Angireddy Rajesh Suresh Yenugu
author_facet	Ganapathy Narmadha Katakam Muneswararao Angireddy Rajesh Suresh Yenugu
author_sort	Ganapathy Narmadha
title	Characterization of a novel lysozyme-like 4 gene in the rat.
title_short	Characterization of a novel lysozyme-like 4 gene in the rat.
title_full	Characterization of a novel lysozyme-like 4 gene in the rat.
title_fullStr	Characterization of a novel lysozyme-like 4 gene in the rat.
title_full_unstemmed	Characterization of a novel lysozyme-like 4 gene in the rat.
title_sort	characterization of a novel lysozyme-like 4 gene in the rat.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/b64fde752b3e47908f2355f06955e6b9
work_keys_str_mv	AT ganapathynarmadha characterizationofanovellysozymelike4geneintherat AT katakammuneswararao characterizationofanovellysozymelike4geneintherat AT angireddyrajesh characterizationofanovellysozymelike4geneintherat AT sureshyenugu characterizationofanovellysozymelike4geneintherat
_version_	1718423309744340992

Characterization of a novel lysozyme-like 4 gene in the rat.

Ejemplares similares