Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin

Amit Kumar, Xinran Li, Michael A Sandoval, B Leticia Rodriguez, Brian R Sloat, Zhengrong CuiUniversity of Texas at Austin, College of Pharmacy, Pharmaceutics Division, Austin, TX, USABackground: The present study was designed to evaluate the extent to which pretreatment with microneedles can enhance...

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Autores principales:	Kumar A, Li X, Sandoval MA, Rodriguez BL, Sloat BR, Cui Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b65488e062c442ff8bdd1f562de1e5c5
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spelling	oai:doaj.org-article:b65488e062c442ff8bdd1f562de1e5c52021-12-02T02:10:26ZPermeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin1176-91141178-2013https://doaj.org/article/b65488e062c442ff8bdd1f562de1e5c52011-06-01T00:00:00Zhttp://www.dovepress.com/permeation-of-antigen-protein-conjugated-nanoparticles-and-live-bacter-a7699https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Amit Kumar, Xinran Li, Michael A Sandoval, B Leticia Rodriguez, Brian R Sloat, Zhengrong CuiUniversity of Texas at Austin, College of Pharmacy, Pharmaceutics Division, Austin, TX, USABackground: The present study was designed to evaluate the extent to which pretreatment with microneedles can enhance skin permeation of nanoparticles in vitro and in vivo. Permeation of live bacteria, which are physically nanoparticles or microparticles, through mouse skin pretreated with microneedles was also studied to evaluate the potential risk of microbial infection.Methods and results: It was found that pretreatment of mouse skin with microneedles allowed permeation of solid lipid nanoparticles, size 230 nm, with ovalbumin conjugated on their surface. Transcutaneous immunization in a mouse skin area pretreated with microneedles with ovalbumin nanoparticles induced a stronger antiovalbumin antibody response than using ovalbumin alone. The dose of ovalbumin antigen determined whether microneedle-mediated transcutaneous immunization with ovalbumin nanoparticles induced a stronger immune response than subcutaneous injection of the same ovalbumin nanoparticles. Microneedle treatment permitted skin permeation of live Escherichia coli, but the extent of the permeation was not greater than that enabled by hypodermic injection.Conclusion: Transcutaneous immunization on a microneedle-treated skin area with antigens carried by nanoparticles can potentially induce a strong immune response, and the risk of bacterial infection associated with microneedle treatment is no greater than that with a hypodermic injection.Keywords: antibody responses, safety of microneedles, transepidermal water lossKumar ALi XSandoval MARodriguez BLSloat BRCui ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1253-1264 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kumar A Li X Sandoval MA Rodriguez BL Sloat BR Cui Z Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin
description	Amit Kumar, Xinran Li, Michael A Sandoval, B Leticia Rodriguez, Brian R Sloat, Zhengrong CuiUniversity of Texas at Austin, College of Pharmacy, Pharmaceutics Division, Austin, TX, USABackground: The present study was designed to evaluate the extent to which pretreatment with microneedles can enhance skin permeation of nanoparticles in vitro and in vivo. Permeation of live bacteria, which are physically nanoparticles or microparticles, through mouse skin pretreated with microneedles was also studied to evaluate the potential risk of microbial infection.Methods and results: It was found that pretreatment of mouse skin with microneedles allowed permeation of solid lipid nanoparticles, size 230 nm, with ovalbumin conjugated on their surface. Transcutaneous immunization in a mouse skin area pretreated with microneedles with ovalbumin nanoparticles induced a stronger antiovalbumin antibody response than using ovalbumin alone. The dose of ovalbumin antigen determined whether microneedle-mediated transcutaneous immunization with ovalbumin nanoparticles induced a stronger immune response than subcutaneous injection of the same ovalbumin nanoparticles. Microneedle treatment permitted skin permeation of live Escherichia coli, but the extent of the permeation was not greater than that enabled by hypodermic injection.Conclusion: Transcutaneous immunization on a microneedle-treated skin area with antigens carried by nanoparticles can potentially induce a strong immune response, and the risk of bacterial infection associated with microneedle treatment is no greater than that with a hypodermic injection.Keywords: antibody responses, safety of microneedles, transepidermal water loss
format	article
author	Kumar A Li X Sandoval MA Rodriguez BL Sloat BR Cui Z
author_facet	Kumar A Li X Sandoval MA Rodriguez BL Sloat BR Cui Z
author_sort	Kumar A
title	Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin
title_short	Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin
title_full	Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin
title_fullStr	Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin
title_full_unstemmed	Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin
title_sort	permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/b65488e062c442ff8bdd1f562de1e5c5
work_keys_str_mv	AT kumara permeationofantigenproteinconjugatednanoparticlesandlivebacteriathroughmicroneedletreatedmouseskin AT lix permeationofantigenproteinconjugatednanoparticlesandlivebacteriathroughmicroneedletreatedmouseskin AT sandovalma permeationofantigenproteinconjugatednanoparticlesandlivebacteriathroughmicroneedletreatedmouseskin AT rodriguezbl permeationofantigenproteinconjugatednanoparticlesandlivebacteriathroughmicroneedletreatedmouseskin AT sloatbr permeationofantigenproteinconjugatednanoparticlesandlivebacteriathroughmicroneedletreatedmouseskin AT cuiz permeationofantigenproteinconjugatednanoparticlesandlivebacteriathroughmicroneedletreatedmouseskin
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Permeation of antigen protein-conjugated nanoparticles and live bacteria through microneedle-treated mouse skin

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