Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.

<h4>Background</h4>To clarify the role of the POU domain transcription factor Oct4 in Adipose Tissue Stromal Cells (ATSCs), we investigated the regulation of Oct4 expression and other embryonic genes in fully differentiated cells, in addition to identifying expression at the gene and pro...

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Autores principales:	Jung Hwan Kim, Min Ki Jee, So Young Lee, Tae Hee Han, Bong Sun Kim, Kyung Sun Kang, Soo Kyung Kang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6572071958945d58f28a6659de872a9
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spelling	oai:doaj.org-article:b6572071958945d58f28a6659de872a92021-11-25T06:20:10ZRegulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.1932-620310.1371/journal.pone.0007166https://doaj.org/article/b6572071958945d58f28a6659de872a92009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19777066/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>To clarify the role of the POU domain transcription factor Oct4 in Adipose Tissue Stromal Cells (ATSCs), we investigated the regulation of Oct4 expression and other embryonic genes in fully differentiated cells, in addition to identifying expression at the gene and protein levels. The ATSCs and several immature cells were routinely expressing Oct4 protein before and after differentiating into specific lineages.<h4>Methodology/principal findings and conclusions</h4>Here, we demonstrated the role of Oct4 in ATSCs on cell proliferation and differentiation. Exogenous Oct4 improves adult ATSCs cell proliferation and differentiation potencies through epigenetic reprogramming of stemness genes such as Oct4, Nanog, Sox2, and Rex1. Oct4 directly or indirectly induces ATSCs reprogramming along with the activation of JAK/STAT3 and ERK1/2. Exogenic Oct4 introduced a transdifferentiation priority into the neural lineage than mesodermal lineage. Global gene expression analysis results showed that Oct4 regulated target genes which could be characterized as differentially regulated genes such as pluripotency markers NANOG, SOX2, and KLF4 and markers of undifferentiated stem cells FOXD1, CDC2, and EPHB1. The negatively regulated genes included FAS, TNFR, COL6A1, JAM2, FOXQ1, FOXO1, NESTIN, SMAD3, SLIT3, DKK1, WNT5A, BMP1, and GLIS3 which are implicated in differentiation processes as well as a number of novel genes. Finally we have demonstrated the therapeutic utility of Oct4/ATSCs were introduced into the mouse traumatic brain, engrafted cells was more effectively induces regeneration activity with high therapeutic modality than that of control ATSCs. Engrafted Oct4/ATSCs efficiently migrated and transdifferentiated into action potential carrying, functionally neurons in the hippocampus and promoting the amelioration of lesion cavities.Jung Hwan KimMin Ki JeeSo Young LeeTae Hee HanBong Sun KimKyung Sun KangSoo Kyung KangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 9, p e7166 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jung Hwan Kim Min Ki Jee So Young Lee Tae Hee Han Bong Sun Kim Kyung Sun Kang Soo Kyung Kang Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.
description	<h4>Background</h4>To clarify the role of the POU domain transcription factor Oct4 in Adipose Tissue Stromal Cells (ATSCs), we investigated the regulation of Oct4 expression and other embryonic genes in fully differentiated cells, in addition to identifying expression at the gene and protein levels. The ATSCs and several immature cells were routinely expressing Oct4 protein before and after differentiating into specific lineages.<h4>Methodology/principal findings and conclusions</h4>Here, we demonstrated the role of Oct4 in ATSCs on cell proliferation and differentiation. Exogenous Oct4 improves adult ATSCs cell proliferation and differentiation potencies through epigenetic reprogramming of stemness genes such as Oct4, Nanog, Sox2, and Rex1. Oct4 directly or indirectly induces ATSCs reprogramming along with the activation of JAK/STAT3 and ERK1/2. Exogenic Oct4 introduced a transdifferentiation priority into the neural lineage than mesodermal lineage. Global gene expression analysis results showed that Oct4 regulated target genes which could be characterized as differentially regulated genes such as pluripotency markers NANOG, SOX2, and KLF4 and markers of undifferentiated stem cells FOXD1, CDC2, and EPHB1. The negatively regulated genes included FAS, TNFR, COL6A1, JAM2, FOXQ1, FOXO1, NESTIN, SMAD3, SLIT3, DKK1, WNT5A, BMP1, and GLIS3 which are implicated in differentiation processes as well as a number of novel genes. Finally we have demonstrated the therapeutic utility of Oct4/ATSCs were introduced into the mouse traumatic brain, engrafted cells was more effectively induces regeneration activity with high therapeutic modality than that of control ATSCs. Engrafted Oct4/ATSCs efficiently migrated and transdifferentiated into action potential carrying, functionally neurons in the hippocampus and promoting the amelioration of lesion cavities.
format	article
author	Jung Hwan Kim Min Ki Jee So Young Lee Tae Hee Han Bong Sun Kim Kyung Sun Kang Soo Kyung Kang
author_facet	Jung Hwan Kim Min Ki Jee So Young Lee Tae Hee Han Bong Sun Kim Kyung Sun Kang Soo Kyung Kang
author_sort	Jung Hwan Kim
title	Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.
title_short	Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.
title_full	Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.
title_fullStr	Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.
title_full_unstemmed	Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.
title_sort	regulation of adipose tissue stromal cells behaviors by endogenic oct4 expression control.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/b6572071958945d58f28a6659de872a9
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_version_	1718413823041339392

Regulation of adipose tissue stromal cells behaviors by endogenic Oct4 expression control.

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