Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration

Abstract We previously reported efficient precision targeted integration of reporter DNA in zebrafish and human cells using CRISPR/Cas9 and short regions of homology. Here, we apply this strategy to isolate zebrafish Cre recombinase drivers whose spatial and temporal restricted expression mimics end...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maira P. Almeida, Jordan M. Welker, Sahiba Siddiqui, Jon Luiken, Stephen C. Ekker, Karl J. Clark, Jeffrey J. Essner, Maura McGrail
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/b65caa7fc4ef4e09a6d1cd7e35dfe5c2
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares