Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).

Interleukin-7 (IL7) is a pleiotropic cytokine that is actively involved in the immune system. The Chinese tree shrew (Tupaia belangeri chinensis) has been proposed as an alternative experimental animal to primates in biomedical research. However, there is a lack of biological knowledge about the imm...

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Autores principales:	Dandan Yu, Ling Xu, Xiao-Hong Liu, Yu Fan, Long-Bao Lü, Yong-Gang Yao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b65e619489514bf3b35e7de69527fa76
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spelling	oai:doaj.org-article:b65e619489514bf3b35e7de69527fa762021-11-18T08:15:05ZDiverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).1932-620310.1371/journal.pone.0099859https://doaj.org/article/b65e619489514bf3b35e7de69527fa762014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24945249/?tool=EBIhttps://doaj.org/toc/1932-6203Interleukin-7 (IL7) is a pleiotropic cytokine that is actively involved in the immune system. The Chinese tree shrew (Tupaia belangeri chinensis) has been proposed as an alternative experimental animal to primates in biomedical research. However, there is a lack of biological knowledge about the immune system of the tree shrew. In this study, we cloned the IL7 gene (tIL7) in the Chinese tree shrew and quantified the expression of mRNA transcripts in eight tissues (heart, liver, spleen, lung, kidney, intestine, skeletal muscle and brain) from 20 individuals. Eleven tIL7 mRNA transcripts were identified in different tissues. The canonical form (tIL7c) had a length of 1817 bp and encoded a predicted gene product with 177 amino acids. Phylogenetic analyses based on the amino acid sequences revealed a considerably large genetic difference between tree shrew and human. Quantification of mRNA expression of transcripts tIL7c, tIL7-sv1, tIL7-sv2 and tIL7-sv3 showed that these transcripts were expressed in all tissues, albeit the expression levels varied in different tissues. Transcripts tIL7c, tIL7-sv1, and tIL7-sv2 had the lowest expression in brain, and tIL7-sv3 had a dramatically high mRNA expression in skeletal muscle and heart. The mRNA expression levels of tIL7c and tIL7-sv1 were significantly increased upon ploy(I:C) stimulation in tree shrew primary renal cells. As with human full-length IL7, tIL7c, tIL7-sv1, tIL7-sv2 and tIL7-sv3 showed similar a subcellular localization pattern. Our results identified diverse tIL7 transcripts in the Chinese tree shrew, which may play a potential role in modulating IL7-regulated biological effects.Dandan YuLing XuXiao-Hong LiuYu FanLong-Bao LüYong-Gang YaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99859 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dandan Yu Ling Xu Xiao-Hong Liu Yu Fan Long-Bao Lü Yong-Gang Yao Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).
description	Interleukin-7 (IL7) is a pleiotropic cytokine that is actively involved in the immune system. The Chinese tree shrew (Tupaia belangeri chinensis) has been proposed as an alternative experimental animal to primates in biomedical research. However, there is a lack of biological knowledge about the immune system of the tree shrew. In this study, we cloned the IL7 gene (tIL7) in the Chinese tree shrew and quantified the expression of mRNA transcripts in eight tissues (heart, liver, spleen, lung, kidney, intestine, skeletal muscle and brain) from 20 individuals. Eleven tIL7 mRNA transcripts were identified in different tissues. The canonical form (tIL7c) had a length of 1817 bp and encoded a predicted gene product with 177 amino acids. Phylogenetic analyses based on the amino acid sequences revealed a considerably large genetic difference between tree shrew and human. Quantification of mRNA expression of transcripts tIL7c, tIL7-sv1, tIL7-sv2 and tIL7-sv3 showed that these transcripts were expressed in all tissues, albeit the expression levels varied in different tissues. Transcripts tIL7c, tIL7-sv1, and tIL7-sv2 had the lowest expression in brain, and tIL7-sv3 had a dramatically high mRNA expression in skeletal muscle and heart. The mRNA expression levels of tIL7c and tIL7-sv1 were significantly increased upon ploy(I:C) stimulation in tree shrew primary renal cells. As with human full-length IL7, tIL7c, tIL7-sv1, tIL7-sv2 and tIL7-sv3 showed similar a subcellular localization pattern. Our results identified diverse tIL7 transcripts in the Chinese tree shrew, which may play a potential role in modulating IL7-regulated biological effects.
format	article
author	Dandan Yu Ling Xu Xiao-Hong Liu Yu Fan Long-Bao Lü Yong-Gang Yao
author_facet	Dandan Yu Ling Xu Xiao-Hong Liu Yu Fan Long-Bao Lü Yong-Gang Yao
author_sort	Dandan Yu
title	Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).
title_short	Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).
title_full	Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).
title_fullStr	Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).
title_full_unstemmed	Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).
title_sort	diverse interleukin-7 mrna transcripts in chinese tree shrew (tupaia belangeri chinensis).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b65e619489514bf3b35e7de69527fa76
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Diverse interleukin-7 mRNA transcripts in Chinese tree shrew (Tupaia belangeri chinensis).

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