Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold

Abstract In this study, single, mix, multilayer Polyvinyl alcohol (PVA) electrospun nanofibers with epidermal growth factor (EGF) and fibroblast growth factor (FGF) were fabricated and characterized as a biological wound dressing scaffolds. The biological activities of the synthesized scaffolds have...

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Autores principales:	Amnah Asiri, Syafiqah Saidin, Mohd Helmi Sani, Rania Hussien Al-Ashwal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b65eb36c0f12434ebc2942f66ed63c21
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spelling	oai:doaj.org-article:b65eb36c0f12434ebc2942f66ed63c212021-12-02T13:20:13ZEpidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold10.1038/s41598-021-85149-x2045-2322https://doaj.org/article/b65eb36c0f12434ebc2942f66ed63c212021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85149-xhttps://doaj.org/toc/2045-2322Abstract In this study, single, mix, multilayer Polyvinyl alcohol (PVA) electrospun nanofibers with epidermal growth factor (EGF) and fibroblast growth factor (FGF) were fabricated and characterized as a biological wound dressing scaffolds. The biological activities of the synthesized scaffolds have been verified by in vitro and in vivo studies. The chemical composition finding showed that the identified functional units within the produced nanofibers (O–H and N–H bonds) are attributed to both growth factors (GFs) in the PVA nanofiber membranes. Electrospun nanofibers' morphological features showed long protrusion and smooth morphology without beads and sprayed with an average range of 198–286 nm fiber diameter. The fiber diameters decrement and the improvement in wettability and surface roughness were recorded after GFs incorporated within the PVA Nanofibers, which indicated potential good adoption as biological dressing scaffolds due to the identified mechanical properties (Young’s modulus) in between 18 and 20 MPa. The MTT assay indicated that the growth factor release from the PVA nanofibers has stimulated cell proliferation and promoted cell viability. In the cell attachment study, the GFs incorporated PVA nanofibers stimulated cell proliferation and adhered better than the PVA control sample and presented no cytotoxic effect. The in vivo studies showed that compared to the control and single PVA-GFs nanofiber, the mix and multilayer scaffolds gave a much more wound reduction at day 7 with better wound repair at day 14–21, which indicated to enhancing tissue regeneration, thus, could be a projected as a suitable burn wound dressing scaffold.Amnah AsiriSyafiqah SaidinMohd Helmi SaniRania Hussien Al-AshwalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amnah Asiri Syafiqah Saidin Mohd Helmi Sani Rania Hussien Al-Ashwal Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold
description	Abstract In this study, single, mix, multilayer Polyvinyl alcohol (PVA) electrospun nanofibers with epidermal growth factor (EGF) and fibroblast growth factor (FGF) were fabricated and characterized as a biological wound dressing scaffolds. The biological activities of the synthesized scaffolds have been verified by in vitro and in vivo studies. The chemical composition finding showed that the identified functional units within the produced nanofibers (O–H and N–H bonds) are attributed to both growth factors (GFs) in the PVA nanofiber membranes. Electrospun nanofibers' morphological features showed long protrusion and smooth morphology without beads and sprayed with an average range of 198–286 nm fiber diameter. The fiber diameters decrement and the improvement in wettability and surface roughness were recorded after GFs incorporated within the PVA Nanofibers, which indicated potential good adoption as biological dressing scaffolds due to the identified mechanical properties (Young’s modulus) in between 18 and 20 MPa. The MTT assay indicated that the growth factor release from the PVA nanofibers has stimulated cell proliferation and promoted cell viability. In the cell attachment study, the GFs incorporated PVA nanofibers stimulated cell proliferation and adhered better than the PVA control sample and presented no cytotoxic effect. The in vivo studies showed that compared to the control and single PVA-GFs nanofiber, the mix and multilayer scaffolds gave a much more wound reduction at day 7 with better wound repair at day 14–21, which indicated to enhancing tissue regeneration, thus, could be a projected as a suitable burn wound dressing scaffold.
format	article
author	Amnah Asiri Syafiqah Saidin Mohd Helmi Sani Rania Hussien Al-Ashwal
author_facet	Amnah Asiri Syafiqah Saidin Mohd Helmi Sani Rania Hussien Al-Ashwal
author_sort	Amnah Asiri
title	Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold
title_short	Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold
title_full	Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold
title_fullStr	Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold
title_full_unstemmed	Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold
title_sort	epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b65eb36c0f12434ebc2942f66ed63c21
work_keys_str_mv	AT amnahasiri epidermalandfibroblastgrowthfactorsincorporatedpolyvinylalcoholelectrospunnanofibersasbiologicaldressingscaffold AT syafiqahsaidin epidermalandfibroblastgrowthfactorsincorporatedpolyvinylalcoholelectrospunnanofibersasbiologicaldressingscaffold AT mohdhelmisani epidermalandfibroblastgrowthfactorsincorporatedpolyvinylalcoholelectrospunnanofibersasbiologicaldressingscaffold AT raniahussienalashwal epidermalandfibroblastgrowthfactorsincorporatedpolyvinylalcoholelectrospunnanofibersasbiologicaldressingscaffold
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Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold

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