Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection

Zhenhua Chen,* Mengen Zhao,* Jie Zhang, Kang Zhou, Xiuli Ren, Xifan Mei Jinzhou Medical University, Jinzhou, 121001, People’s Republic of China *These authors contributed equally to this work Introduction: Treatment of infection within bone is difficult, and conventional surgical treatm...

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Autores principales:	Chen Z, Zhao M, Zhang J, Zhou K, Ren X, Mei X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Osteomyelitis oxidative stress L-Lysine micro-medicine delivery vehicle Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b6647f11c4414bd483736b37c62a0b36
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spelling	oai:doaj.org-article:b6647f11c4414bd483736b37c62a0b362021-12-02T00:19:16ZConstruction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection1178-2013https://doaj.org/article/b6647f11c4414bd483736b37c62a0b362018-06-01T00:00:00Zhttps://www.dovepress.com/construction-of-injectable-ph-sensitive-antibacterial-mineralized-amin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhenhua Chen,* Mengen Zhao,* Jie Zhang, Kang Zhou, Xiuli Ren, Xifan Mei Jinzhou Medical University, Jinzhou, 121001, People’s Republic of China *These authors contributed equally to this work Introduction: Treatment of infection within bone is difficult, and conventional surgical treatment brings intense pain to the patients physically and mentally. There is an urgent need to develop injectable nano- and/or micro-medicine for minimally invasive treatment of osteomyelitis. Methods: In this paper, amino acid (L-lysine [Lys]) was mineralized into yolk-shell structured CaCO3 microspheres (MSs). The morphologies of the obtained MSs were investigated by scanning electron microscopy and transmission electron microscopy. The composition of CaCO3 MSs was identified by using Fourier transform infrared spectroscopy. The as-prepared CaCO3 MSs were examined with power X-ray diffraction analysis to obtain the crystallographic structure of the MSs. Results: The as prepared Lys encapsulated CaCO3 MSs (Lys@CaCO3 MSs) were used as micro-drug to improve acidic environment of osteomyelitis caused by bacterial infection and promote osteoblast proliferation under oxidative stress. These pH responsive Lys@CaCO3 MSs have a drug loading efficiency of 89.8 wt % and drug loading content (DLC) of 22.3 wt %. Conclusion: Our results demonstrated that Lys@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5. Keywords: osteomyelitis, oxidative stress, L-Lysine, micro-medicine, delivery vehicleChen ZZhao MZhang JZhou KRen XMei XDove Medical PressarticleOsteomyelitisoxidative stressL-Lysinemicro-medicinedelivery vehicleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3493-3506 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteomyelitis oxidative stress L-Lysine micro-medicine delivery vehicle Medicine (General) R5-920
spellingShingle	Osteomyelitis oxidative stress L-Lysine micro-medicine delivery vehicle Medicine (General) R5-920 Chen Z Zhao M Zhang J Zhou K Ren X Mei X Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection
description	Zhenhua Chen,* Mengen Zhao,* Jie Zhang, Kang Zhou, Xiuli Ren, Xifan Mei Jinzhou Medical University, Jinzhou, 121001, People’s Republic of China *These authors contributed equally to this work Introduction: Treatment of infection within bone is difficult, and conventional surgical treatment brings intense pain to the patients physically and mentally. There is an urgent need to develop injectable nano- and/or micro-medicine for minimally invasive treatment of osteomyelitis. Methods: In this paper, amino acid (L-lysine [Lys]) was mineralized into yolk-shell structured CaCO3 microspheres (MSs). The morphologies of the obtained MSs were investigated by scanning electron microscopy and transmission electron microscopy. The composition of CaCO3 MSs was identified by using Fourier transform infrared spectroscopy. The as-prepared CaCO3 MSs were examined with power X-ray diffraction analysis to obtain the crystallographic structure of the MSs. Results: The as prepared Lys encapsulated CaCO3 MSs (Lys@CaCO3 MSs) were used as micro-drug to improve acidic environment of osteomyelitis caused by bacterial infection and promote osteoblast proliferation under oxidative stress. These pH responsive Lys@CaCO3 MSs have a drug loading efficiency of 89.8 wt % and drug loading content (DLC) of 22.3 wt %. Conclusion: Our results demonstrated that Lys@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5. Keywords: osteomyelitis, oxidative stress, L-Lysine, micro-medicine, delivery vehicle
format	article
author	Chen Z Zhao M Zhang J Zhou K Ren X Mei X
author_facet	Chen Z Zhao M Zhang J Zhou K Ren X Mei X
author_sort	Chen Z
title	Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection
title_short	Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection
title_full	Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection
title_fullStr	Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection
title_full_unstemmed	Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection
title_sort	construction of injectable, ph sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/b6647f11c4414bd483736b37c62a0b36
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Construction of injectable, pH sensitive, antibacterial, mineralized amino acid yolk-shell microspheres for potential minimally invasive treatment of bone infection

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