Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro

Abstract Breast cancer is the leading cause of cancer death among women worldwide. Like other cancers, mammary carcinoma progression involves acidification of the tumor microenvironment, which is an important factor for cancer detection and treatment strategies. However, the effects of acidity on ma...

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Autores principales:	Ana Carolina Lima Ralph, Iuri Cordeiro Valadão, Elaine Cristina Cardoso, Vilma Regina Martins, Luanda Mara Silva Oliveira, Estela Maris Andrade Forell Bevilacqua, Murilo Vieira Geraldo, Ruy Gastaldoni Jaeger, Gary S. Goldberg, Vanessa Morais Freitas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6671f43e829449cb1e2ca4d8aaeaab4
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spelling	oai:doaj.org-article:b6671f43e829449cb1e2ca4d8aaeaab42021-12-02T12:40:40ZEnvironmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro10.1038/s41598-020-78989-62045-2322https://doaj.org/article/b6671f43e829449cb1e2ca4d8aaeaab42020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78989-6https://doaj.org/toc/2045-2322Abstract Breast cancer is the leading cause of cancer death among women worldwide. Like other cancers, mammary carcinoma progression involves acidification of the tumor microenvironment, which is an important factor for cancer detection and treatment strategies. However, the effects of acidity on mammary carcinoma cell morphology and phenotype have not been thoroughly characterized. Here, we evaluated fundamental effects of environmental acidification on mammary carcinoma cells in standard two-dimensional cultures and three-dimensional spheroids. Acidification decreased overall mammary carcinoma cell viability, while increasing their resistance to the anthracycline doxorubicin. Environmental acidification also increased extracellular vesicle production by mammary carcinoma cells. Conditioned media containing these vesicles appeared to increase fibroblast motility. Acidification also increased mammary carcinoma cell motility when cultured with fibroblasts in spheroids. Taken together, results from this study suggest that environmental acidification induces drug resistance and extracellular vesicle production by mammary carcinoma cells that promote tumor expansion.Ana Carolina Lima RalphIuri Cordeiro ValadãoElaine Cristina CardosoVilma Regina MartinsLuanda Mara Silva OliveiraEstela Maris Andrade Forell BevilacquaMurilo Vieira GeraldoRuy Gastaldoni JaegerGary S. GoldbergVanessa Morais FreitasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Carolina Lima Ralph Iuri Cordeiro Valadão Elaine Cristina Cardoso Vilma Regina Martins Luanda Mara Silva Oliveira Estela Maris Andrade Forell Bevilacqua Murilo Vieira Geraldo Ruy Gastaldoni Jaeger Gary S. Goldberg Vanessa Morais Freitas Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro
description	Abstract Breast cancer is the leading cause of cancer death among women worldwide. Like other cancers, mammary carcinoma progression involves acidification of the tumor microenvironment, which is an important factor for cancer detection and treatment strategies. However, the effects of acidity on mammary carcinoma cell morphology and phenotype have not been thoroughly characterized. Here, we evaluated fundamental effects of environmental acidification on mammary carcinoma cells in standard two-dimensional cultures and three-dimensional spheroids. Acidification decreased overall mammary carcinoma cell viability, while increasing their resistance to the anthracycline doxorubicin. Environmental acidification also increased extracellular vesicle production by mammary carcinoma cells. Conditioned media containing these vesicles appeared to increase fibroblast motility. Acidification also increased mammary carcinoma cell motility when cultured with fibroblasts in spheroids. Taken together, results from this study suggest that environmental acidification induces drug resistance and extracellular vesicle production by mammary carcinoma cells that promote tumor expansion.
format	article
author	Ana Carolina Lima Ralph Iuri Cordeiro Valadão Elaine Cristina Cardoso Vilma Regina Martins Luanda Mara Silva Oliveira Estela Maris Andrade Forell Bevilacqua Murilo Vieira Geraldo Ruy Gastaldoni Jaeger Gary S. Goldberg Vanessa Morais Freitas
author_facet	Ana Carolina Lima Ralph Iuri Cordeiro Valadão Elaine Cristina Cardoso Vilma Regina Martins Luanda Mara Silva Oliveira Estela Maris Andrade Forell Bevilacqua Murilo Vieira Geraldo Ruy Gastaldoni Jaeger Gary S. Goldberg Vanessa Morais Freitas
author_sort	Ana Carolina Lima Ralph
title	Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro
title_short	Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro
title_full	Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro
title_fullStr	Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro
title_full_unstemmed	Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro
title_sort	environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b6671f43e829449cb1e2ca4d8aaeaab4
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Environmental control of mammary carcinoma cell expansion by acidification and spheroid formation in vitro

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