Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection

Plants have been used since long time ago to treat infectious diseases and are considered as important sources of new antimicrobial agents. In this study, crude extracts from leaves of Conyza bonariensis were prepared using methanol, ethyl acetate, hexane, and chloroform. Antimicrobial activity of t...

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Autores principales:	Yidnekachew Girma, Tamene Milkessa Jiru
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b66842cd0cfd4b1eac7eba61f3d26073
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spelling	oai:doaj.org-article:b66842cd0cfd4b1eac7eba61f3d260732021-11-15T01:19:49ZEvaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection2090-907110.1155/2021/6367449https://doaj.org/article/b66842cd0cfd4b1eac7eba61f3d260732021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/6367449https://doaj.org/toc/2090-9071Plants have been used since long time ago to treat infectious diseases and are considered as important sources of new antimicrobial agents. In this study, crude extracts from leaves of Conyza bonariensis were prepared using methanol, ethyl acetate, hexane, and chloroform. Antimicrobial activity of the extracts was evaluated against pathogenic fungi causing superficial infection (Candida albicans, Malassezia globosa, and Malassezia furfur). Results demonstrated that all extracts had different effects against all the tested fungi with the exception of crude extract using hexane which did not show any effect against M. furfur. A strong effect was observed with chloroform and hexane extracts on C. albicans (32.60 ± 4.69 mm and 27.00 ± 1.00 mm), respectively. While, ethyl acetate and methanol extracts showed the best effect against M. furfur (30.80 ± 1.71 mm and 27.00 ± 1.00 mm), respectively. Moreover, the ethyl acetate showed a considerable effect on M. globosa (25.03 ± 1.05 mm). Minimum inhibitory concentration (MIC) of the fractions was also determined by the microbroth dilution method. The results recorded as the MIC values of the tested extracts against fungi varied from 0.19 ± 0.00 to 66.66 ± 2.86 mg/mL. Ethyl acetate was the best and powerful extract with the lowest MIC value of 0.190.19 ± 0.00 mg/mL for all tested fungi followed by chloroform and methanol extracts with the MIC values ranging from 0.19 ± 0.00 to 0.78 ± 0.00 mg/mL and 0.84 ± 0.68 to 1.56 mg/mL, respectively. Concerning minimum bactericidal concentration (MFC), ethyl acetate was the most potent extract with a MFC value of 0.190.19 ± 0.00 mg/mL for C. albicans and M. furfur. Higher (0.39 mg/mL) MFC was recorded against M. globosa by this extract. In conclusion, solvent extracts of some C. bonariensis can be used to treat infections with pathogenic fungi such as C. albicans, M. furfur, and M. globosa. Further studies should consider this plant as one of the best candidates for the discovery of potent antimicrobial compounds that treat superficial infections.Yidnekachew GirmaTamene Milkessa JiruHindawi LimitedarticleChemistryQD1-999ENJournal of Chemistry, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chemistry QD1-999
spellingShingle	Chemistry QD1-999 Yidnekachew Girma Tamene Milkessa Jiru Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection
description	Plants have been used since long time ago to treat infectious diseases and are considered as important sources of new antimicrobial agents. In this study, crude extracts from leaves of Conyza bonariensis were prepared using methanol, ethyl acetate, hexane, and chloroform. Antimicrobial activity of the extracts was evaluated against pathogenic fungi causing superficial infection (Candida albicans, Malassezia globosa, and Malassezia furfur). Results demonstrated that all extracts had different effects against all the tested fungi with the exception of crude extract using hexane which did not show any effect against M. furfur. A strong effect was observed with chloroform and hexane extracts on C. albicans (32.60 ± 4.69 mm and 27.00 ± 1.00 mm), respectively. While, ethyl acetate and methanol extracts showed the best effect against M. furfur (30.80 ± 1.71 mm and 27.00 ± 1.00 mm), respectively. Moreover, the ethyl acetate showed a considerable effect on M. globosa (25.03 ± 1.05 mm). Minimum inhibitory concentration (MIC) of the fractions was also determined by the microbroth dilution method. The results recorded as the MIC values of the tested extracts against fungi varied from 0.19 ± 0.00 to 66.66 ± 2.86 mg/mL. Ethyl acetate was the best and powerful extract with the lowest MIC value of 0.190.19 ± 0.00 mg/mL for all tested fungi followed by chloroform and methanol extracts with the MIC values ranging from 0.19 ± 0.00 to 0.78 ± 0.00 mg/mL and 0.84 ± 0.68 to 1.56 mg/mL, respectively. Concerning minimum bactericidal concentration (MFC), ethyl acetate was the most potent extract with a MFC value of 0.190.19 ± 0.00 mg/mL for C. albicans and M. furfur. Higher (0.39 mg/mL) MFC was recorded against M. globosa by this extract. In conclusion, solvent extracts of some C. bonariensis can be used to treat infections with pathogenic fungi such as C. albicans, M. furfur, and M. globosa. Further studies should consider this plant as one of the best candidates for the discovery of potent antimicrobial compounds that treat superficial infections.
format	article
author	Yidnekachew Girma Tamene Milkessa Jiru
author_facet	Yidnekachew Girma Tamene Milkessa Jiru
author_sort	Yidnekachew Girma
title	Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection
title_short	Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection
title_full	Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection
title_fullStr	Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection
title_full_unstemmed	Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection
title_sort	evaluation of antimicrobial activity of conyza bonariensis leaf extracts against clinically isolated fungi causing superficial infection
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/b66842cd0cfd4b1eac7eba61f3d26073
work_keys_str_mv	AT yidnekachewgirma evaluationofantimicrobialactivityofconyzabonariensisleafextractsagainstclinicallyisolatedfungicausingsuperficialinfection AT tamenemilkessajiru evaluationofantimicrobialactivityofconyzabonariensisleafextractsagainstclinicallyisolatedfungicausingsuperficialinfection
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Evaluation of Antimicrobial Activity of Conyza bonariensis Leaf Extracts against Clinically Isolated Fungi Causing Superficial Infection

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