From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy

For twenty-five years, attempts have been made to use MSCs in the treatment of various diseases due to their regenerative and immunomodulatory properties. However, the results are not satisfactory. Assuming that MSCs can be replaced in some therapies by the active factors they produce, the immortali...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria Paprocka, Honorata Kraskiewicz, Aleksandra Bielawska-Pohl, Agnieszka Krawczenko, Leszek Masłowski, Agnieszka Czyżewska-Buczyńska, Wojciech Witkiewicz, Danuta Dus, Anna Czarnecka
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipose tissue origin mesenchymal stem/stromal cells HATMSC1 immortalized cell line cytokine production Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b668c22bd5e444d4bba64aabead19f1a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b668c22bd5e444d4bba64aabead19f1a
record_format	dspace
spelling	oai:doaj.org-article:b668c22bd5e444d4bba64aabead19f1a2021-11-11T16:54:15ZFrom Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy10.3390/ijms2221114391422-00671661-6596https://doaj.org/article/b668c22bd5e444d4bba64aabead19f1a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11439https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067For twenty-five years, attempts have been made to use MSCs in the treatment of various diseases due to their regenerative and immunomodulatory properties. However, the results are not satisfactory. Assuming that MSCs can be replaced in some therapies by the active factors they produce, the immortalized MSCs line was established from human adipose tissue (HATMSC1) to produce conditioned media and test its regenerative potential in vitro in terms of possible clinical application. The production of biologically active factors by primary MSCs was lower compared to the HATMSC1 cell line and several factors were produced only by the cell line. It has been shown that an HATMSC1-conditioned medium increases the proliferation of various cell types, augments the adhesion of cells and improves endothelial cell function. It was found that hypoxia during culture resulted in an augmentation in the pro-angiogenic factors production, such as VEGF, IL-8, Angiogenin and MCP-1. The immunomodulatory factors caused an increase in the production of GM-CSF, IL-5, IL-6, MCP-1, RANTES and IL-8. These data suggest that these factors, produced under different culture conditions, could be used for different medical conditions, such as in regenerative medicine, when an increased concentration of pro-angiogenic factors may be beneficial, or in inflammatory diseases with conditioned media with a high concentration of immunomodulatory factors.Maria PaprockaHonorata KraskiewiczAleksandra Bielawska-PohlAgnieszka KrawczenkoLeszek MasłowskiAgnieszka Czyżewska-BuczyńskaWojciech WitkiewiczDanuta DusAnna CzarneckaMDPI AGarticleadipose tissue origin mesenchymal stem/stromal cellsHATMSC1immortalized cell linecytokine productionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11439, p 11439 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose tissue origin mesenchymal stem/stromal cells HATMSC1 immortalized cell line cytokine production Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	adipose tissue origin mesenchymal stem/stromal cells HATMSC1 immortalized cell line cytokine production Biology (General) QH301-705.5 Chemistry QD1-999 Maria Paprocka Honorata Kraskiewicz Aleksandra Bielawska-Pohl Agnieszka Krawczenko Leszek Masłowski Agnieszka Czyżewska-Buczyńska Wojciech Witkiewicz Danuta Dus Anna Czarnecka From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy
description	For twenty-five years, attempts have been made to use MSCs in the treatment of various diseases due to their regenerative and immunomodulatory properties. However, the results are not satisfactory. Assuming that MSCs can be replaced in some therapies by the active factors they produce, the immortalized MSCs line was established from human adipose tissue (HATMSC1) to produce conditioned media and test its regenerative potential in vitro in terms of possible clinical application. The production of biologically active factors by primary MSCs was lower compared to the HATMSC1 cell line and several factors were produced only by the cell line. It has been shown that an HATMSC1-conditioned medium increases the proliferation of various cell types, augments the adhesion of cells and improves endothelial cell function. It was found that hypoxia during culture resulted in an augmentation in the pro-angiogenic factors production, such as VEGF, IL-8, Angiogenin and MCP-1. The immunomodulatory factors caused an increase in the production of GM-CSF, IL-5, IL-6, MCP-1, RANTES and IL-8. These data suggest that these factors, produced under different culture conditions, could be used for different medical conditions, such as in regenerative medicine, when an increased concentration of pro-angiogenic factors may be beneficial, or in inflammatory diseases with conditioned media with a high concentration of immunomodulatory factors.
format	article
author	Maria Paprocka Honorata Kraskiewicz Aleksandra Bielawska-Pohl Agnieszka Krawczenko Leszek Masłowski Agnieszka Czyżewska-Buczyńska Wojciech Witkiewicz Danuta Dus Anna Czarnecka
author_facet	Maria Paprocka Honorata Kraskiewicz Aleksandra Bielawska-Pohl Agnieszka Krawczenko Leszek Masłowski Agnieszka Czyżewska-Buczyńska Wojciech Witkiewicz Danuta Dus Anna Czarnecka
author_sort	Maria Paprocka
title	From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy
title_short	From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy
title_full	From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy
title_fullStr	From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy
title_full_unstemmed	From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy
title_sort	from primary msc culture of adipose tissue to immortalized cell line producing cytokines for potential use in regenerative medicine therapy or immunotherapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b668c22bd5e444d4bba64aabead19f1a
work_keys_str_mv	AT mariapaprocka fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT honoratakraskiewicz fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT aleksandrabielawskapohl fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT agnieszkakrawczenko fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT leszekmasłowski fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT agnieszkaczyzewskabuczynska fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT wojciechwitkiewicz fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT danutadus fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy AT annaczarnecka fromprimarymsccultureofadiposetissuetoimmortalizedcelllineproducingcytokinesforpotentialuseinregenerativemedicinetherapyorimmunotherapy
_version_	1718432198404603904

From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy

Ejemplares similares