Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol
In order to improve the capability of Klebsiella pneumoniae to produce an important chemical raw material, 1,3-propanediol (1,3-PDO), a new type of K. pneumoniae x546 was obtained by glycerol acclimation and subsequently was used to produce 1,3-PDO. Under the control of pH value using Na+ pH neutral...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b668d6dc2a2f4441b00f15dab2008df1 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b668d6dc2a2f4441b00f15dab2008df1 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b668d6dc2a2f4441b00f15dab2008df12021-12-01T07:29:20ZRational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol1664-302X10.3389/fmicb.2021.770109https://doaj.org/article/b668d6dc2a2f4441b00f15dab2008df12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.770109/fullhttps://doaj.org/toc/1664-302XIn order to improve the capability of Klebsiella pneumoniae to produce an important chemical raw material, 1,3-propanediol (1,3-PDO), a new type of K. pneumoniae x546 was obtained by glycerol acclimation and subsequently was used to produce 1,3-PDO. Under the control of pH value using Na+ pH neutralizer, the 1,3-PDO yield of K. pneumoniae x546 in a 7.5-L fermenter was 69.35 g/L, which was 1.5-fold higher than the original strain (45.91 g/L). After the addition of betaine, the yield of 1,3-PDO reached up to 74.44 g/L at 24 h, which was 40% shorter than the original fermentation time of 40 h. To study the potential mechanism of the production improvement of 1,3-PDO, the Tandem Mass Tags (TMT) technology was applied to investigate the production of 1,3-PDO in K. pneumoniae. Compared with the control group, 170 up-regulated proteins and 291 down-regulated proteins were identified. Through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, it was found that some proteins [such as homoserine kinase (ThrB), phosphoribosylglycinamide formyltransferase (PurT), phosphoribosylaminoimidazolesuccinocarboxamide synthase (PurC), etc.] were involved in the fermentation process, whereas some other proteins (such as ProX, ProW, ProV, etc.) played a significant role after the addition of betaine. Moreover, combined with the metabolic network of K. pneumoniae during 1,3-PDO, the proteins in the biosynthesis of 1,3-PDO [such as DhaD, DhaK, lactate dehydrogenase (LDH), BudC, etc.] were analyzed. The process of 1,3-PDO production in K. pneumoniae was explained from the perspective of proteome for the first time, which provided a theoretical basis for genetic engineering modification to improve the yield of 1,3-PDO. Because of the use of Na+ pH neutralizer in the fermentation, the subsequent environmental pollution treatment cost was greatly reduced, showing high potential for industry application in the future.Xin WangXin WangXin WangXin WangLin ZhangHong ChenHong ChenPan WangPan WangYing YinYing YinJiaqi JinJiaqi JinJianwei XuJianwei XuJianping WenJianping WenFrontiers Media S.A.articleKlebsiella pneumoniae13-propanediol productionbetaineNa+ pH neutralizerproteomicsMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Klebsiella pneumoniae 1 3-propanediol production betaine Na+ pH neutralizer proteomics Microbiology QR1-502 |
| spellingShingle |
Klebsiella pneumoniae 1 3-propanediol production betaine Na+ pH neutralizer proteomics Microbiology QR1-502 Xin Wang Xin Wang Xin Wang Xin Wang Lin Zhang Hong Chen Hong Chen Pan Wang Pan Wang Ying Yin Ying Yin Jiaqi Jin Jiaqi Jin Jianwei Xu Jianwei Xu Jianping Wen Jianping Wen Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol |
| description |
In order to improve the capability of Klebsiella pneumoniae to produce an important chemical raw material, 1,3-propanediol (1,3-PDO), a new type of K. pneumoniae x546 was obtained by glycerol acclimation and subsequently was used to produce 1,3-PDO. Under the control of pH value using Na+ pH neutralizer, the 1,3-PDO yield of K. pneumoniae x546 in a 7.5-L fermenter was 69.35 g/L, which was 1.5-fold higher than the original strain (45.91 g/L). After the addition of betaine, the yield of 1,3-PDO reached up to 74.44 g/L at 24 h, which was 40% shorter than the original fermentation time of 40 h. To study the potential mechanism of the production improvement of 1,3-PDO, the Tandem Mass Tags (TMT) technology was applied to investigate the production of 1,3-PDO in K. pneumoniae. Compared with the control group, 170 up-regulated proteins and 291 down-regulated proteins were identified. Through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, it was found that some proteins [such as homoserine kinase (ThrB), phosphoribosylglycinamide formyltransferase (PurT), phosphoribosylaminoimidazolesuccinocarboxamide synthase (PurC), etc.] were involved in the fermentation process, whereas some other proteins (such as ProX, ProW, ProV, etc.) played a significant role after the addition of betaine. Moreover, combined with the metabolic network of K. pneumoniae during 1,3-PDO, the proteins in the biosynthesis of 1,3-PDO [such as DhaD, DhaK, lactate dehydrogenase (LDH), BudC, etc.] were analyzed. The process of 1,3-PDO production in K. pneumoniae was explained from the perspective of proteome for the first time, which provided a theoretical basis for genetic engineering modification to improve the yield of 1,3-PDO. Because of the use of Na+ pH neutralizer in the fermentation, the subsequent environmental pollution treatment cost was greatly reduced, showing high potential for industry application in the future. |
| format |
article |
| author |
Xin Wang Xin Wang Xin Wang Xin Wang Lin Zhang Hong Chen Hong Chen Pan Wang Pan Wang Ying Yin Ying Yin Jiaqi Jin Jiaqi Jin Jianwei Xu Jianwei Xu Jianping Wen Jianping Wen |
| author_facet |
Xin Wang Xin Wang Xin Wang Xin Wang Lin Zhang Hong Chen Hong Chen Pan Wang Pan Wang Ying Yin Ying Yin Jiaqi Jin Jiaqi Jin Jianwei Xu Jianwei Xu Jianping Wen Jianping Wen |
| author_sort |
Xin Wang |
| title |
Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol |
| title_short |
Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol |
| title_full |
Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol |
| title_fullStr |
Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol |
| title_full_unstemmed |
Rational Proteomic Analysis of a New Domesticated Klebsiella pneumoniae x546 Producing 1,3-Propanediol |
| title_sort |
rational proteomic analysis of a new domesticated klebsiella pneumoniae x546 producing 1,3-propanediol |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/b668d6dc2a2f4441b00f15dab2008df1 |
| work_keys_str_mv |
AT xinwang rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT xinwang rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT xinwang rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT xinwang rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT linzhang rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT hongchen rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT hongchen rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT panwang rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT panwang rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT yingyin rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT yingyin rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT jiaqijin rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT jiaqijin rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT jianweixu rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT jianweixu rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT jianpingwen rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol AT jianpingwen rationalproteomicanalysisofanewdomesticatedklebsiellapneumoniaex546producing13propanediol |
| _version_ |
1718405435903442944 |