From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.

The predator-prey interactions within food chains are used to both characterize and understand ecosystems. Conventional methods of constructing food chains from visual identification of prey in predator diet can suffer from poor taxonomic resolution, misidentification, and bias against small or comp...

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Autores principales:	A Kirsten Bowser, Antony W Diamond, Jason A Addison
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b67297bce632466b8fbe74403caa65ae
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spelling	oai:doaj.org-article:b67297bce632466b8fbe74403caa65ae2021-11-18T08:41:45ZFrom puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.1932-620310.1371/journal.pone.0083152https://doaj.org/article/b67297bce632466b8fbe74403caa65ae2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24358258/?tool=EBIhttps://doaj.org/toc/1932-6203The predator-prey interactions within food chains are used to both characterize and understand ecosystems. Conventional methods of constructing food chains from visual identification of prey in predator diet can suffer from poor taxonomic resolution, misidentification, and bias against small or completely digestible prey. Next-generation sequencing (NGS) technology has become a powerful tool for diet reconstruction through barcoding of DNA in stomach content or fecal samples. Here we use multi-locus (16S and CO1) next-generation sequencing of DNA barcodes on the feces of Atlantic puffin (Fratercula arctica) chicks (n=65) and adults (n=64) and the stomach contents of their main prey, Atlantic herring (Clupea harengus, n=44) to investigate a previously studied food chain. We compared conventional and molecular-derived chick diet, tested the similarity between the diets of puffin adults and chicks, and determined whether herring prey can be detected in puffin diet samples. There was high variability in the coverage of prey groups between 16S and CO1 markers. We identified more unique prey with our 16S compared to CO1 barcoding markers (51 and 39 taxa respectively) with only 12 taxa identified by both genes. We found no significant difference between the 16S-identified diets of puffin adults (n=17) and chicks (n=41). Our molecular method is more taxonomically resolved and detected chick prey at higher frequencies than conventional field observations. Many likely planktonic prey of herring were detected in feces from puffin adults and chicks, highlighting the impact secondary consumption may have on the interpretation of molecular dietary analysis. This study represents the first simultaneous molecular investigation into the diet of multiple components of a food chain and highlights the utility of a multi-locus approach to diet reconstruction that is broadly applicable to food web analysis.A Kirsten BowserAntony W DiamondJason A AddisonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83152 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A Kirsten Bowser Antony W Diamond Jason A Addison From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.
description	The predator-prey interactions within food chains are used to both characterize and understand ecosystems. Conventional methods of constructing food chains from visual identification of prey in predator diet can suffer from poor taxonomic resolution, misidentification, and bias against small or completely digestible prey. Next-generation sequencing (NGS) technology has become a powerful tool for diet reconstruction through barcoding of DNA in stomach content or fecal samples. Here we use multi-locus (16S and CO1) next-generation sequencing of DNA barcodes on the feces of Atlantic puffin (Fratercula arctica) chicks (n=65) and adults (n=64) and the stomach contents of their main prey, Atlantic herring (Clupea harengus, n=44) to investigate a previously studied food chain. We compared conventional and molecular-derived chick diet, tested the similarity between the diets of puffin adults and chicks, and determined whether herring prey can be detected in puffin diet samples. There was high variability in the coverage of prey groups between 16S and CO1 markers. We identified more unique prey with our 16S compared to CO1 barcoding markers (51 and 39 taxa respectively) with only 12 taxa identified by both genes. We found no significant difference between the 16S-identified diets of puffin adults (n=17) and chicks (n=41). Our molecular method is more taxonomically resolved and detected chick prey at higher frequencies than conventional field observations. Many likely planktonic prey of herring were detected in feces from puffin adults and chicks, highlighting the impact secondary consumption may have on the interpretation of molecular dietary analysis. This study represents the first simultaneous molecular investigation into the diet of multiple components of a food chain and highlights the utility of a multi-locus approach to diet reconstruction that is broadly applicable to food web analysis.
format	article
author	A Kirsten Bowser Antony W Diamond Jason A Addison
author_facet	A Kirsten Bowser Antony W Diamond Jason A Addison
author_sort	A Kirsten Bowser
title	From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.
title_short	From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.
title_full	From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.
title_fullStr	From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.
title_full_unstemmed	From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.
title_sort	from puffins to plankton: a dna-based analysis of a seabird food chain in the northern gulf of maine.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b67297bce632466b8fbe74403caa65ae
work_keys_str_mv	AT akirstenbowser frompuffinstoplanktonadnabasedanalysisofaseabirdfoodchaininthenortherngulfofmaine AT antonywdiamond frompuffinstoplanktonadnabasedanalysisofaseabirdfoodchaininthenortherngulfofmaine AT jasonaaddison frompuffinstoplanktonadnabasedanalysisofaseabirdfoodchaininthenortherngulfofmaine
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From puffins to plankton: a DNA-based analysis of a seabird food chain in the northern Gulf of Maine.

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