Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader

Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW sampl...

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Autores principales:	Hisashi Satoh, Yutaka Katayose, Reiko Hirano
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	enzyme fecal pollution fluctuation fluorescence sapporo wastewater Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/b67340af0aba466dad275f2d51c30c79
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spelling	oai:doaj.org-article:b67340af0aba466dad275f2d51c30c792021-11-06T10:52:35ZSimple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader0273-12231996-973210.2166/wst.2021.072https://doaj.org/article/b67340af0aba466dad275f2d51c30c792021-03-01T00:00:00Zhttp://wst.iwaponline.com/content/83/6/1399https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the β-d-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at >100 most probable numbers 100 mL−1 with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW. HIGHLIGHTS A novel method was applied for enumeration of Escherichia coli in urban rivers.; Response time was <3 h and detection limit was 430 most probable numbers 100 mL−1.; Specific enzyme activities of E. coli were similar in wastewater and river waters.; Correlation between enzyme activity and E. coli concentration was site independent.; E. coli concentrations were monitored at eight different sites over 9 months.;Hisashi SatohYutaka KatayoseReiko HiranoIWA Publishingarticleenzymefecal pollutionfluctuationfluorescencesapporowastewaterEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 6, Pp 1399-1406 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	enzyme fecal pollution fluctuation fluorescence sapporo wastewater Environmental technology. Sanitary engineering TD1-1066
spellingShingle	enzyme fecal pollution fluctuation fluorescence sapporo wastewater Environmental technology. Sanitary engineering TD1-1066 Hisashi Satoh Yutaka Katayose Reiko Hirano Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
description	Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the β-d-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at >100 most probable numbers 100 mL−1 with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW. HIGHLIGHTS A novel method was applied for enumeration of Escherichia coli in urban rivers.; Response time was <3 h and detection limit was 430 most probable numbers 100 mL−1.; Specific enzyme activities of E. coli were similar in wastewater and river waters.; Correlation between enzyme activity and E. coli concentration was site independent.; E. coli concentrations were monitored at eight different sites over 9 months.;
format	article
author	Hisashi Satoh Yutaka Katayose Reiko Hirano
author_facet	Hisashi Satoh Yutaka Katayose Reiko Hirano
author_sort	Hisashi Satoh
title	Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
title_short	Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
title_full	Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
title_fullStr	Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
title_full_unstemmed	Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
title_sort	simple enumeration of escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/b67340af0aba466dad275f2d51c30c79
work_keys_str_mv	AT hisashisatoh simpleenumerationofescherichiacoliconcentrationsinriverwatersamplesbymeasuringbdglucuronidaseactivitiesinamicroplatereader AT yutakakatayose simpleenumerationofescherichiacoliconcentrationsinriverwatersamplesbymeasuringbdglucuronidaseactivitiesinamicroplatereader AT reikohirano simpleenumerationofescherichiacoliconcentrationsinriverwatersamplesbymeasuringbdglucuronidaseactivitiesinamicroplatereader
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Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader

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