Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis

Yuji Komizu, Hidetsugu Ueoka, Koichi Goto, Ryuichi UeokaDivision of Applied Life Science, Graduate School of Engineering, Sojo University, Ikeda, Kumamoto, JapanBackground: Hybrid liposomes can be prepared by simply sonicating a mixture of vesicular and micellar molecules in buffer solutions. In thi...

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Autores principales:	Goto K, Ueoka R, Komizu Y, Ueoka H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b67bdaf293eb4676b471997808be0b1a
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spelling	oai:doaj.org-article:b67bdaf293eb4676b471997808be0b1a2021-12-02T02:04:17ZRemarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis1176-91141178-2013https://doaj.org/article/b67bdaf293eb4676b471997808be0b1a2011-09-01T00:00:00Zhttp://www.dovepress.com/remarkable-inhibitory-effects-of-hybrid-liposomes-on-growth-of-human-c-a8250https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yuji Komizu, Hidetsugu Ueoka, Koichi Goto, Ryuichi UeokaDivision of Applied Life Science, Graduate School of Engineering, Sojo University, Ikeda, Kumamoto, JapanBackground: Hybrid liposomes can be prepared by simply sonicating a mixture of vesicular and micellar molecules in buffer solutions. In this study, we investigated the effects of hybrid liposomes on the growth of human colon cancer cells in vitro.Methods: Hybrid liposomes (HL-n, n = 21, 23, 25) composed of L-&alpha;-dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene(n) dodecyl ethers (C12(EO)n, n = 21, 23, 25) were prepared by the sonication method and their inhibitory effects on growth of human colon cancer HCT116 cells were examined in vitro.Results: Significant growth inhibition of HCT116 cells was observed in the presence of HL-n. The fifty percent inhibitory concentration (IC50) of HL-n was less than half that of DMPC liposomes. Furthermore, fluorescence microscopic and flow cytometric analyses indicated that the markedly inhibitory effects of HL-n on the growth of HCT116 cells could be attained through the induction of cell cycle arrest at the G0/G1 phase along with apoptotic cell death.Conclusion: It was found for the first time that HL-n can induce both cell cycle arrest and apoptosis in colon cancer cells. The findings in this study should contribute to novel chemotherapy for colon cancer.Keywords: hybrid liposome, colon cancer cell, cell cycle arrest, apoptosisGoto KUeoka RKomizu YUeoka HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1913-1920 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Goto K Ueoka R Komizu Y Ueoka H Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis
description	Yuji Komizu, Hidetsugu Ueoka, Koichi Goto, Ryuichi UeokaDivision of Applied Life Science, Graduate School of Engineering, Sojo University, Ikeda, Kumamoto, JapanBackground: Hybrid liposomes can be prepared by simply sonicating a mixture of vesicular and micellar molecules in buffer solutions. In this study, we investigated the effects of hybrid liposomes on the growth of human colon cancer cells in vitro.Methods: Hybrid liposomes (HL-n, n = 21, 23, 25) composed of L-&alpha;-dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene(n) dodecyl ethers (C12(EO)n, n = 21, 23, 25) were prepared by the sonication method and their inhibitory effects on growth of human colon cancer HCT116 cells were examined in vitro.Results: Significant growth inhibition of HCT116 cells was observed in the presence of HL-n. The fifty percent inhibitory concentration (IC50) of HL-n was less than half that of DMPC liposomes. Furthermore, fluorescence microscopic and flow cytometric analyses indicated that the markedly inhibitory effects of HL-n on the growth of HCT116 cells could be attained through the induction of cell cycle arrest at the G0/G1 phase along with apoptotic cell death.Conclusion: It was found for the first time that HL-n can induce both cell cycle arrest and apoptosis in colon cancer cells. The findings in this study should contribute to novel chemotherapy for colon cancer.Keywords: hybrid liposome, colon cancer cell, cell cycle arrest, apoptosis
format	article
author	Goto K Ueoka R Komizu Y Ueoka H
author_facet	Goto K Ueoka R Komizu Y Ueoka H
author_sort	Goto K
title	Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis
title_short	Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis
title_full	Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis
title_fullStr	Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis
title_full_unstemmed	Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis
title_sort	remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/b67bdaf293eb4676b471997808be0b1a
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Remarkable inhibitory effects of hybrid liposomes on growth of human colon cancer cells through induction of cell cycle arrest along with apoptosis

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