Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants

Abstract Ralstonia pseudosolanacearum GMI1000 (Rpso GMI1000) is a soil-borne vascular phytopathogen that infects host plants through the root system causing wilting disease in a wide range of agro-economic interest crops, producing economical losses. Several features contribute to the full bacterial...

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Autores principales:	Josefina Tano, María Belén Ripa, María Laura Tondo, Analía Carrau, Silvana Petrocelli, María Victoria Rodriguez, Virginia Ferreira, María Inés Siri, Laura Piskulic, Elena Graciela Orellano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b67becf877774516ab61466bbc3dfe39
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spelling	oai:doaj.org-article:b67becf877774516ab61466bbc3dfe392021-12-02T16:14:02ZLight modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants10.1038/s41598-021-93871-92045-2322https://doaj.org/article/b67becf877774516ab61466bbc3dfe392021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93871-9https://doaj.org/toc/2045-2322Abstract Ralstonia pseudosolanacearum GMI1000 (Rpso GMI1000) is a soil-borne vascular phytopathogen that infects host plants through the root system causing wilting disease in a wide range of agro-economic interest crops, producing economical losses. Several features contribute to the full bacterial virulence. In this work we study the participation of light, an important environmental factor, in the regulation of the physiological attributes and infectivity of Rpso GMI1000. In silico analysis of the Rpso genome revealed the presence of a Rsp0254 gene, which encodes a putative blue light LOV-type photoreceptor. We constructed a mutant strain of Rpso lacking the LOV protein and found that the loss of this protein and light, influenced characteristics involved in the pathogenicity process such as motility, adhesion and the biofilms development, which allows the successful host plant colonization, rendering bacterial wilt. This protein could be involved in the adaptive responses to environmental changes. We demonstrated that light sensing and the LOV protein, would be used as a location signal in the host plant, to regulate the expression of several virulence factors, in a time and tissue dependent way. Consequently, bacteria could use an external signal and Rpsolov gene to know their location within plant tissue during the colonization process.Josefina TanoMaría Belén RipaMaría Laura TondoAnalía CarrauSilvana PetrocelliMaría Victoria RodriguezVirginia FerreiraMaría Inés SiriLaura PiskulicElena Graciela OrellanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Josefina Tano María Belén Ripa María Laura Tondo Analía Carrau Silvana Petrocelli María Victoria Rodriguez Virginia Ferreira María Inés Siri Laura Piskulic Elena Graciela Orellano Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants
description	Abstract Ralstonia pseudosolanacearum GMI1000 (Rpso GMI1000) is a soil-borne vascular phytopathogen that infects host plants through the root system causing wilting disease in a wide range of agro-economic interest crops, producing economical losses. Several features contribute to the full bacterial virulence. In this work we study the participation of light, an important environmental factor, in the regulation of the physiological attributes and infectivity of Rpso GMI1000. In silico analysis of the Rpso genome revealed the presence of a Rsp0254 gene, which encodes a putative blue light LOV-type photoreceptor. We constructed a mutant strain of Rpso lacking the LOV protein and found that the loss of this protein and light, influenced characteristics involved in the pathogenicity process such as motility, adhesion and the biofilms development, which allows the successful host plant colonization, rendering bacterial wilt. This protein could be involved in the adaptive responses to environmental changes. We demonstrated that light sensing and the LOV protein, would be used as a location signal in the host plant, to regulate the expression of several virulence factors, in a time and tissue dependent way. Consequently, bacteria could use an external signal and Rpsolov gene to know their location within plant tissue during the colonization process.
format	article
author	Josefina Tano María Belén Ripa María Laura Tondo Analía Carrau Silvana Petrocelli María Victoria Rodriguez Virginia Ferreira María Inés Siri Laura Piskulic Elena Graciela Orellano
author_facet	Josefina Tano María Belén Ripa María Laura Tondo Analía Carrau Silvana Petrocelli María Victoria Rodriguez Virginia Ferreira María Inés Siri Laura Piskulic Elena Graciela Orellano
author_sort	Josefina Tano
title	Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants
title_short	Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants
title_full	Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants
title_fullStr	Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants
title_full_unstemmed	Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants
title_sort	light modulates important physiological features of ralstonia pseudosolanacearum during the colonization of tomato plants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b67becf877774516ab61466bbc3dfe39
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Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants

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