Depth-dependent differences in community structure of the human colonic microbiota in health.
<h4>Objective</h4>The aims of this study were to develop techniques for spatial microbial assessment in humans and to establish colonic luminal and mucosal spatial ecology, encompassing longitudinal and cross-sectional axes.<h4>Design</h4>A microbiological protected specimen...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b681a9f9e0c048bb9efebfac0a437464 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b681a9f9e0c048bb9efebfac0a437464 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b681a9f9e0c048bb9efebfac0a4374642021-11-18T08:48:06ZDepth-dependent differences in community structure of the human colonic microbiota in health.1932-620310.1371/journal.pone.0078835https://doaj.org/article/b681a9f9e0c048bb9efebfac0a4374642013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24223167/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>The aims of this study were to develop techniques for spatial microbial assessment in humans and to establish colonic luminal and mucosal spatial ecology, encompassing longitudinal and cross-sectional axes.<h4>Design</h4>A microbiological protected specimen brush was used in conjunction with a biopsy forceps to sample the colon in nine healthy volunteers undergoing colonoscopy. Terminal Restriction Fragment Length Polymorphism analysis was used to determine the major variables in the spatial organization of the colonic microbiota.<h4>Results</h4>Protected Specimen Brush sampling retrieved region-specific, uncontaminated samples that were enriched for bacterial DNA and depleted in human DNA when compared to biopsy samples. Terminal Restriction Fragment Length Polymorphism analysis revealed a segmentation of bacterial communities between the luminal brush and biopsy-associated ecological niches with little variability across the longitudinal axis of the colon and reduced diversity in brush samples.<h4>Conclusion</h4>These results support the concept of a microbiota with little longitudinal variability but with some degree of segregation between luminal and mucosal communities.Aonghus LavelleGrainne LennonNeil DochertyAine BalfeHugh E MulcahyGlen DohertyDiarmuid O DonoghueJohn M HylandFergus ShanahanKieran SheahanJ Calvin CoffeyDesmond C WinterP Ronan O ConnellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e78835 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Aonghus Lavelle Grainne Lennon Neil Docherty Aine Balfe Hugh E Mulcahy Glen Doherty Diarmuid O Donoghue John M Hyland Fergus Shanahan Kieran Sheahan J Calvin Coffey Desmond C Winter P Ronan O Connell Depth-dependent differences in community structure of the human colonic microbiota in health. |
| description |
<h4>Objective</h4>The aims of this study were to develop techniques for spatial microbial assessment in humans and to establish colonic luminal and mucosal spatial ecology, encompassing longitudinal and cross-sectional axes.<h4>Design</h4>A microbiological protected specimen brush was used in conjunction with a biopsy forceps to sample the colon in nine healthy volunteers undergoing colonoscopy. Terminal Restriction Fragment Length Polymorphism analysis was used to determine the major variables in the spatial organization of the colonic microbiota.<h4>Results</h4>Protected Specimen Brush sampling retrieved region-specific, uncontaminated samples that were enriched for bacterial DNA and depleted in human DNA when compared to biopsy samples. Terminal Restriction Fragment Length Polymorphism analysis revealed a segmentation of bacterial communities between the luminal brush and biopsy-associated ecological niches with little variability across the longitudinal axis of the colon and reduced diversity in brush samples.<h4>Conclusion</h4>These results support the concept of a microbiota with little longitudinal variability but with some degree of segregation between luminal and mucosal communities. |
| format |
article |
| author |
Aonghus Lavelle Grainne Lennon Neil Docherty Aine Balfe Hugh E Mulcahy Glen Doherty Diarmuid O Donoghue John M Hyland Fergus Shanahan Kieran Sheahan J Calvin Coffey Desmond C Winter P Ronan O Connell |
| author_facet |
Aonghus Lavelle Grainne Lennon Neil Docherty Aine Balfe Hugh E Mulcahy Glen Doherty Diarmuid O Donoghue John M Hyland Fergus Shanahan Kieran Sheahan J Calvin Coffey Desmond C Winter P Ronan O Connell |
| author_sort |
Aonghus Lavelle |
| title |
Depth-dependent differences in community structure of the human colonic microbiota in health. |
| title_short |
Depth-dependent differences in community structure of the human colonic microbiota in health. |
| title_full |
Depth-dependent differences in community structure of the human colonic microbiota in health. |
| title_fullStr |
Depth-dependent differences in community structure of the human colonic microbiota in health. |
| title_full_unstemmed |
Depth-dependent differences in community structure of the human colonic microbiota in health. |
| title_sort |
depth-dependent differences in community structure of the human colonic microbiota in health. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/b681a9f9e0c048bb9efebfac0a437464 |
| work_keys_str_mv |
AT aonghuslavelle depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT grainnelennon depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT neildocherty depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT ainebalfe depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT hughemulcahy depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT glendoherty depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT diarmuidodonoghue depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT johnmhyland depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT fergusshanahan depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT kieransheahan depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT jcalvincoffey depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT desmondcwinter depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth AT pronanoconnell depthdependentdifferencesincommunitystructureofthehumancolonicmicrobiotainhealth |
| _version_ |
1718421283568353280 |