The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens

Functional genetic screens by CRISPR-Cas9 allow for the unbiased discovery of proteins causally involved in complex biological processes. In recent years, this approach has been used by multiple laboratories to uncover a range of tumor cell regulators determining immune sensitivity. In this review,...

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Autores principales:	D.W. Vredevoogd, G. Apriamashvili, D.S. Peeper
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	immunotherapy immune checkpoint blockade CRISPR-Cas9 genetic screen therapy resistance Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/b6875c84503849e3804aa00e191cb1ad
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spelling	oai:doaj.org-article:b6875c84503849e3804aa00e191cb1ad2021-12-04T04:35:39ZThe (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens2590-018810.1016/j.iotech.2021.100043https://doaj.org/article/b6875c84503849e3804aa00e191cb1ad2021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590018821000186https://doaj.org/toc/2590-0188Functional genetic screens by CRISPR-Cas9 allow for the unbiased discovery of proteins causally involved in complex biological processes. In recent years, this approach has been used by multiple laboratories to uncover a range of tumor cell regulators determining immune sensitivity. In this review, we provide an overview of genetic screens carried out both in vitro and in vivo. By comparative analysis we highlight commonly identified proteins and pathways that are key in establishing tumor-intrinsic immune susceptibility. Together, these screens demonstrated the importance of the antigen presentation, interferon-γ, tumor necrosis factor and autophagy pathways in governing sensitivity of tumor cells to immune attack. Moreover, they underline the complex interplay between tumor cells and their microenvironment, providing both fundamental and clinically relevant insights into the mechanisms of tumor immune resistance.D.W. VredevoogdG. ApriamashviliD.S. PeeperElsevierarticleimmunotherapyimmune checkpoint blockadeCRISPR-Cas9genetic screentherapy resistanceNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENImmuno-Oncology and Technology, Vol 11, Iss , Pp 100043- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunotherapy immune checkpoint blockade CRISPR-Cas9 genetic screen therapy resistance Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	immunotherapy immune checkpoint blockade CRISPR-Cas9 genetic screen therapy resistance Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 D.W. Vredevoogd G. Apriamashvili D.S. Peeper The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens
description	Functional genetic screens by CRISPR-Cas9 allow for the unbiased discovery of proteins causally involved in complex biological processes. In recent years, this approach has been used by multiple laboratories to uncover a range of tumor cell regulators determining immune sensitivity. In this review, we provide an overview of genetic screens carried out both in vitro and in vivo. By comparative analysis we highlight commonly identified proteins and pathways that are key in establishing tumor-intrinsic immune susceptibility. Together, these screens demonstrated the importance of the antigen presentation, interferon-γ, tumor necrosis factor and autophagy pathways in governing sensitivity of tumor cells to immune attack. Moreover, they underline the complex interplay between tumor cells and their microenvironment, providing both fundamental and clinically relevant insights into the mechanisms of tumor immune resistance.
format	article
author	D.W. Vredevoogd G. Apriamashvili D.S. Peeper
author_facet	D.W. Vredevoogd G. Apriamashvili D.S. Peeper
author_sort	D.W. Vredevoogd
title	The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens
title_short	The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens
title_full	The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens
title_fullStr	The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens
title_full_unstemmed	The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens
title_sort	(re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/b6875c84503849e3804aa00e191cb1ad
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The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens

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