Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction

The present study aims to investigate the NALP3 system and its influence on occludin in Sertoli cells, utilising primary murine cells and adult Sertoli cell line as models. Its main goals are the Sertoli cell biology with possible implications on male reproductive functions. Primary and adult Sertol...

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Autores principales:	Hayrabedyan S., Todorova K.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	sertoli cells tight-junctions occludin nalp3 Medicine R
Acceso en línea:	https://doaj.org/article/b6876b965a0b44d1b6090b9cafafd5ed
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spelling	oai:doaj.org-article:b6876b965a0b44d1b6090b9cafafd5ed2021-12-02T19:11:12ZNalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction0324-175010.1515/amb-2015-0002https://doaj.org/article/b6876b965a0b44d1b6090b9cafafd5ed2015-05-01T00:00:00Zhttps://doi.org/10.1515/amb-2015-0002https://doaj.org/toc/0324-1750The present study aims to investigate the NALP3 system and its influence on occludin in Sertoli cells, utilising primary murine cells and adult Sertoli cell line as models. Its main goals are the Sertoli cell biology with possible implications on male reproductive functions. Primary and adult Sertoli cells were transfected with NAPL3 siRNA and treated with NOD1 (ie-DAP) and NOD2 (MDP) receptor ligands. There was positive occludin expression levels on transcript (RT-qPCR) and protein (FCS and Immunofluorescence) levels for both cell types. The innate immunity and tight-junction pathways integration serve a protective role for both testis immune barrier and spermatogenesis compartmentalisation maintained by the very same barrier. This integration also points the way for mechanistic research of the disturbances inflicted during an inflammatory response in testis niche.Hayrabedyan S.Todorova K.Sciendoarticlesertoli cellstight-junctionsoccludinnalp3MedicineRENActa Medica Bulgarica, Vol 42, Iss 1, Pp 12-17 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	sertoli cells tight-junctions occludin nalp3 Medicine R
spellingShingle	sertoli cells tight-junctions occludin nalp3 Medicine R Hayrabedyan S. Todorova K. Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction
description	The present study aims to investigate the NALP3 system and its influence on occludin in Sertoli cells, utilising primary murine cells and adult Sertoli cell line as models. Its main goals are the Sertoli cell biology with possible implications on male reproductive functions. Primary and adult Sertoli cells were transfected with NAPL3 siRNA and treated with NOD1 (ie-DAP) and NOD2 (MDP) receptor ligands. There was positive occludin expression levels on transcript (RT-qPCR) and protein (FCS and Immunofluorescence) levels for both cell types. The innate immunity and tight-junction pathways integration serve a protective role for both testis immune barrier and spermatogenesis compartmentalisation maintained by the very same barrier. This integration also points the way for mechanistic research of the disturbances inflicted during an inflammatory response in testis niche.
format	article
author	Hayrabedyan S. Todorova K.
author_facet	Hayrabedyan S. Todorova K.
author_sort	Hayrabedyan S.
title	Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction
title_short	Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction
title_full	Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction
title_fullStr	Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction
title_full_unstemmed	Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction
title_sort	nalp signalling is required in sertoli cells for tight-junction protein interaction
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/b6876b965a0b44d1b6090b9cafafd5ed
work_keys_str_mv	AT hayrabedyans nalpsignallingisrequiredinsertolicellsfortightjunctionproteininteraction AT todorovak nalpsignallingisrequiredinsertolicellsfortightjunctionproteininteraction
_version_	1718377111412015104

Nalp Signalling is Required in Sertoli Cells for Tight-Junction Protein Interaction

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