In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia

Abstract The ability to genetically manipulate trigeminal ganglion (TG) neurons would be useful in the study of the craniofacial nervous system and latent alphaherpesvirus infections. We investigated adeno-associated virus (AAV) vectors for gene delivery to the TG after intradermal whiskerpad delive...

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Autores principales:	Chung H. Dang, Martine Aubert, Harshana S. De Silva Feelixge, Kurt Diem, Michelle A. Loprieno, Pavitra Roychoudhury, Daniel Stone, Keith R. Jerome
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b68a60e4420041afb93d1660c83fed5e
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spelling	oai:doaj.org-article:b68a60e4420041afb93d1660c83fed5e2021-12-02T16:07:49ZIn vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia10.1038/s41598-017-01004-y2045-2322https://doaj.org/article/b68a60e4420041afb93d1660c83fed5e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01004-yhttps://doaj.org/toc/2045-2322Abstract The ability to genetically manipulate trigeminal ganglion (TG) neurons would be useful in the study of the craniofacial nervous system and latent alphaherpesvirus infections. We investigated adeno-associated virus (AAV) vectors for gene delivery to the TG after intradermal whiskerpad delivery in mice. We demonstrated that AAV vectors of serotypes 1, 7, 8, and 9 trafficked from the whiskerpad into TG neurons and expressed transgenes within cell bodies and axons of sensory neurons in all three branches of the TG. Gene expression was highest with AAV1, and steadily increased over time up to day 28. Both constitutive and neuronal-specific promoters were able to drive transgene expression in TG neurons. Levels of vector genomes in the TG increased with input dose, and multiple transgenes could be co-delivered to TG neurons by separate AAV vectors. In conclusion, AAV1 vectors are suitable for gene delivery to TG sensory neurons following intradermal whiskerpad injection.Chung H. DangMartine AubertHarshana S. De Silva FeelixgeKurt DiemMichelle A. LoprienoPavitra RoychoudhuryDaniel StoneKeith R. JeromeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chung H. Dang Martine Aubert Harshana S. De Silva Feelixge Kurt Diem Michelle A. Loprieno Pavitra Roychoudhury Daniel Stone Keith R. Jerome In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia
description	Abstract The ability to genetically manipulate trigeminal ganglion (TG) neurons would be useful in the study of the craniofacial nervous system and latent alphaherpesvirus infections. We investigated adeno-associated virus (AAV) vectors for gene delivery to the TG after intradermal whiskerpad delivery in mice. We demonstrated that AAV vectors of serotypes 1, 7, 8, and 9 trafficked from the whiskerpad into TG neurons and expressed transgenes within cell bodies and axons of sensory neurons in all three branches of the TG. Gene expression was highest with AAV1, and steadily increased over time up to day 28. Both constitutive and neuronal-specific promoters were able to drive transgene expression in TG neurons. Levels of vector genomes in the TG increased with input dose, and multiple transgenes could be co-delivered to TG neurons by separate AAV vectors. In conclusion, AAV1 vectors are suitable for gene delivery to TG sensory neurons following intradermal whiskerpad injection.
format	article
author	Chung H. Dang Martine Aubert Harshana S. De Silva Feelixge Kurt Diem Michelle A. Loprieno Pavitra Roychoudhury Daniel Stone Keith R. Jerome
author_facet	Chung H. Dang Martine Aubert Harshana S. De Silva Feelixge Kurt Diem Michelle A. Loprieno Pavitra Roychoudhury Daniel Stone Keith R. Jerome
author_sort	Chung H. Dang
title	In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia
title_short	In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia
title_full	In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia
title_fullStr	In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia
title_full_unstemmed	In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia
title_sort	in vivo dynamics of aav-mediated gene delivery to sensory neurons of the trigeminal ganglia
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b68a60e4420041afb93d1660c83fed5e
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In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia

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