Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta

Abstract TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single rec...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Vishal Dinkar, S. K. Jha, Niharika Mallick, M. Niranjana, Priyanka Agarwal, J. B. Sharma, Vinod
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
R
Q
Acceso en línea:https://doaj.org/article/b68efded6f28408288fd20d04695a16c
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:b68efded6f28408288fd20d04695a16c
record_format dspace
spelling oai:doaj.org-article:b68efded6f28408288fd20d04695a16c2021-12-02T13:34:10ZMolecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta10.1038/s41598-020-78679-32045-2322https://doaj.org/article/b68efded6f28408288fd20d04695a16c2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78679-3https://doaj.org/toc/2045-2322Abstract TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs 276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs 276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs 276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat.Vishal DinkarS. K. JhaNiharika MallickM. NiranjanaPriyanka AgarwalJ. B. SharmaVinodNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Vishal Dinkar
S. K. Jha
Niharika Mallick
M. Niranjana
Priyanka Agarwal
J. B. Sharma
Vinod
Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
description Abstract TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs 276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs 276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs 276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat.
format article
author Vishal Dinkar
S. K. Jha
Niharika Mallick
M. Niranjana
Priyanka Agarwal
J. B. Sharma
Vinod
author_facet Vishal Dinkar
S. K. Jha
Niharika Mallick
M. Niranjana
Priyanka Agarwal
J. B. Sharma
Vinod
author_sort Vishal Dinkar
title Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
title_short Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
title_full Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
title_fullStr Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
title_full_unstemmed Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
title_sort molecular mapping of a new recessive wheat leaf rust resistance gene originating from triticum spelta
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/b68efded6f28408288fd20d04695a16c
work_keys_str_mv AT vishaldinkar molecularmappingofanewrecessivewheatleafrustresistancegeneoriginatingfromtriticumspelta
AT skjha molecularmappingofanewrecessivewheatleafrustresistancegeneoriginatingfromtriticumspelta
AT niharikamallick molecularmappingofanewrecessivewheatleafrustresistancegeneoriginatingfromtriticumspelta
AT mniranjana molecularmappingofanewrecessivewheatleafrustresistancegeneoriginatingfromtriticumspelta
AT priyankaagarwal molecularmappingofanewrecessivewheatleafrustresistancegeneoriginatingfromtriticumspelta
AT jbsharma molecularmappingofanewrecessivewheatleafrustresistancegeneoriginatingfromtriticumspelta
AT vinod molecularmappingofanewrecessivewheatleafrustresistancegeneoriginatingfromtriticumspelta
_version_ 1718392816510435328