Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta
Abstract TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single rec...
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oai:doaj.org-article:b68efded6f28408288fd20d04695a16c2021-12-02T13:34:10ZMolecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta10.1038/s41598-020-78679-32045-2322https://doaj.org/article/b68efded6f28408288fd20d04695a16c2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78679-3https://doaj.org/toc/2045-2322Abstract TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs 276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs 276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs 276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat.Vishal DinkarS. K. JhaNiharika MallickM. NiranjanaPriyanka AgarwalJ. B. SharmaVinodNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
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Medicine R Science Q Vishal Dinkar S. K. Jha Niharika Mallick M. Niranjana Priyanka Agarwal J. B. Sharma Vinod Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta |
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Abstract TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F1, F2, F2:3 and BC1F1 generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs 276-2, in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs 276-2 in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs 276-2 is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat. |
format |
article |
author |
Vishal Dinkar S. K. Jha Niharika Mallick M. Niranjana Priyanka Agarwal J. B. Sharma Vinod |
author_facet |
Vishal Dinkar S. K. Jha Niharika Mallick M. Niranjana Priyanka Agarwal J. B. Sharma Vinod |
author_sort |
Vishal Dinkar |
title |
Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta |
title_short |
Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta |
title_full |
Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta |
title_fullStr |
Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta |
title_full_unstemmed |
Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta |
title_sort |
molecular mapping of a new recessive wheat leaf rust resistance gene originating from triticum spelta |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/b68efded6f28408288fd20d04695a16c |
work_keys_str_mv |
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