Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles

Abstract Metal nanoparticles can either quench or enhance the emission of dyes in their vicinity, but the precise measurement and understanding of this effect is still hindered by experimental artifacts, especially for particles in colloidal dispersion. Here, we introduce a new methodology to correc...

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Autores principales:	Tânia Ribeiro, Carlos Baleizão, José Paulo S. Farinha
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b69319d7ea8e4f59b60c8324135e942f
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spelling	oai:doaj.org-article:b69319d7ea8e4f59b60c8324135e942f2021-12-02T15:05:10ZArtefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles10.1038/s41598-017-02678-02045-2322https://doaj.org/article/b69319d7ea8e4f59b60c8324135e942f2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02678-0https://doaj.org/toc/2045-2322Abstract Metal nanoparticles can either quench or enhance the emission of dyes in their vicinity, but the precise measurement and understanding of this effect is still hindered by experimental artifacts, especially for particles in colloidal dispersion. Here, we introduce a new methodology to correct the inner filter effect of the metal on the dye emission. To test the method, we developed new hybrid nanoparticles with a gold core and a silica shell of precise thickness (tuned from 7 to 13 nm), with a high quantum yield perylenediimide dye on the surface. This novel approach effectively avoids fluorescence quenching, allowing us to measure emission enhancements of 5 to 30 times, with no change on the dye fluorescence lifetime. Being able to measure the emission enhancement in dye-metal hybrid nanoparticles in dispersion, free from inner filter and quenching artifacts, offers excellent prospects to guide the development of more efficient fluorescent probes, sensors and photonic devices.Tânia RibeiroCarlos BaleizãoJosé Paulo S. FarinhaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tânia Ribeiro Carlos Baleizão José Paulo S. Farinha Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles
description	Abstract Metal nanoparticles can either quench or enhance the emission of dyes in their vicinity, but the precise measurement and understanding of this effect is still hindered by experimental artifacts, especially for particles in colloidal dispersion. Here, we introduce a new methodology to correct the inner filter effect of the metal on the dye emission. To test the method, we developed new hybrid nanoparticles with a gold core and a silica shell of precise thickness (tuned from 7 to 13 nm), with a high quantum yield perylenediimide dye on the surface. This novel approach effectively avoids fluorescence quenching, allowing us to measure emission enhancements of 5 to 30 times, with no change on the dye fluorescence lifetime. Being able to measure the emission enhancement in dye-metal hybrid nanoparticles in dispersion, free from inner filter and quenching artifacts, offers excellent prospects to guide the development of more efficient fluorescent probes, sensors and photonic devices.
format	article
author	Tânia Ribeiro Carlos Baleizão José Paulo S. Farinha
author_facet	Tânia Ribeiro Carlos Baleizão José Paulo S. Farinha
author_sort	Tânia Ribeiro
title	Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles
title_short	Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles
title_full	Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles
title_fullStr	Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles
title_full_unstemmed	Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles
title_sort	artefact-free evaluation of metal enhanced fluorescence in silica coated gold nanoparticles
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b69319d7ea8e4f59b60c8324135e942f
work_keys_str_mv	AT taniaribeiro artefactfreeevaluationofmetalenhancedfluorescenceinsilicacoatedgoldnanoparticles AT carlosbaleizao artefactfreeevaluationofmetalenhancedfluorescenceinsilicacoatedgoldnanoparticles AT josepaulosfarinha artefactfreeevaluationofmetalenhancedfluorescenceinsilicacoatedgoldnanoparticles
_version_	1718388917134163968

Artefact-free Evaluation of Metal Enhanced Fluorescence in Silica Coated Gold Nanoparticles

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