Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus

Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus

Abstract In this paper, we present the optical characterisations of diabetic red blood cells (RBCs) in a non-invasive manner employing three-dimensional (3-D) quantitative phase imaging. By measuring 3-D refractive index tomograms and 2-D time-series phase images, the morphological (volume, surface...

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Autores principales: SangYun Lee, HyunJoo Park, Kyoohyun Kim, YongHak Sohn, Seongsoo Jang, YongKeun Park
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/b698d677d34949218e5e661e23835702
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spelling oai:doaj.org-article:b698d677d34949218e5e661e238357022021-12-02T16:07:05ZRefractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus10.1038/s41598-017-01036-42045-2322https://doaj.org/article/b698d677d34949218e5e661e238357022017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01036-4https://doaj.org/toc/2045-2322Abstract In this paper, we present the optical characterisations of diabetic red blood cells (RBCs) in a non-invasive manner employing three-dimensional (3-D) quantitative phase imaging. By measuring 3-D refractive index tomograms and 2-D time-series phase images, the morphological (volume, surface area and sphericity), biochemical (haemoglobin concentration and content) and mechanical (membrane fluctuation) parameters were quantitatively retrieved at the individual cell level. With simultaneous measurements of individual cell properties, systematic correlative analyses on retrieved RBC parameters were also performed. Our measurements show there exist no statistically significant alterations in morphological and biochemical parameters of diabetic RBCs, compared to those of healthy (non-diabetic) RBCs. In contrast, membrane deformability of diabetic RBCs is significantly lower than that of healthy, non-diabetic RBCs. Interestingly, non-diabetic RBCs exhibit strong correlations between the elevated glycated haemoglobin in RBC cytoplasm and decreased cell deformability, whereas diabetic RBCs do not show correlations. Our observations strongly support the idea that slow and irreversible glycation of haemoglobin and membrane proteins of RBCs by hyperglycaemia significantly compromises RBC deformability in diabetic patients.SangYun LeeHyunJoo ParkKyoohyun KimYongHak SohnSeongsoo JangYongKeun ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
SangYun Lee
HyunJoo Park
Kyoohyun Kim
YongHak Sohn
Seongsoo Jang
YongKeun Park
Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
description Abstract In this paper, we present the optical characterisations of diabetic red blood cells (RBCs) in a non-invasive manner employing three-dimensional (3-D) quantitative phase imaging. By measuring 3-D refractive index tomograms and 2-D time-series phase images, the morphological (volume, surface area and sphericity), biochemical (haemoglobin concentration and content) and mechanical (membrane fluctuation) parameters were quantitatively retrieved at the individual cell level. With simultaneous measurements of individual cell properties, systematic correlative analyses on retrieved RBC parameters were also performed. Our measurements show there exist no statistically significant alterations in morphological and biochemical parameters of diabetic RBCs, compared to those of healthy (non-diabetic) RBCs. In contrast, membrane deformability of diabetic RBCs is significantly lower than that of healthy, non-diabetic RBCs. Interestingly, non-diabetic RBCs exhibit strong correlations between the elevated glycated haemoglobin in RBC cytoplasm and decreased cell deformability, whereas diabetic RBCs do not show correlations. Our observations strongly support the idea that slow and irreversible glycation of haemoglobin and membrane proteins of RBCs by hyperglycaemia significantly compromises RBC deformability in diabetic patients.
format article
author SangYun Lee
HyunJoo Park
Kyoohyun Kim
YongHak Sohn
Seongsoo Jang
YongKeun Park
author_facet SangYun Lee
HyunJoo Park
Kyoohyun Kim
YongHak Sohn
Seongsoo Jang
YongKeun Park
author_sort SangYun Lee
title Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
title_short Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
title_full Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
title_fullStr Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
title_full_unstemmed Refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
title_sort refractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/b698d677d34949218e5e661e23835702
work_keys_str_mv AT sangyunlee refractiveindextomogramsanddynamicmembranefluctuationsofredbloodcellsfrompatientswithdiabetesmellitus
AT hyunjoopark refractiveindextomogramsanddynamicmembranefluctuationsofredbloodcellsfrompatientswithdiabetesmellitus
AT kyoohyunkim refractiveindextomogramsanddynamicmembranefluctuationsofredbloodcellsfrompatientswithdiabetesmellitus
AT yonghaksohn refractiveindextomogramsanddynamicmembranefluctuationsofredbloodcellsfrompatientswithdiabetesmellitus
AT seongsoojang refractiveindextomogramsanddynamicmembranefluctuationsofredbloodcellsfrompatientswithdiabetesmellitus
AT yongkeunpark refractiveindextomogramsanddynamicmembranefluctuationsofredbloodcellsfrompatientswithdiabetesmellitus
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