Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography

Abstract Mixed‐mode chromatography (MMC) is an interesting technique for challenging protein separation processes which typically combines adsorption mechanisms of ion exchange (IEC) and hydrophobic interaction chromatography (HIC). Adsorption equilibria in MMC depend on multiple parameters but syst...

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Autores principales:	Jannette Kreusser, Fabian Jirasek, Hans Hasse
Formato:	article
Lenguaje:	EN
Publicado:	Wiley-VCH 2021
Materias:	adsorption isotherms chromatography modeling pH protein Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b69dddd71cd14aa7a8a0dc0eb7af9ec3
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spelling	oai:doaj.org-article:b69dddd71cd14aa7a8a0dc0eb7af9ec32021-11-09T05:07:05ZInfluence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography1618-28631618-024010.1002/elsc.202100058https://doaj.org/article/b69dddd71cd14aa7a8a0dc0eb7af9ec32021-11-01T00:00:00Zhttps://doi.org/10.1002/elsc.202100058https://doaj.org/toc/1618-0240https://doaj.org/toc/1618-2863Abstract Mixed‐mode chromatography (MMC) is an interesting technique for challenging protein separation processes which typically combines adsorption mechanisms of ion exchange (IEC) and hydrophobic interaction chromatography (HIC). Adsorption equilibria in MMC depend on multiple parameters but systematic studies on their influence are scarce. In the present work, the influence of the pH value and ionic strengths up to 3000 mM of four technically relevant salts (sodium chloride, sodium sulfate, ammonium chloride, and ammonium sulfate) on the lysozyme adsorption on the mixed‐mode resin Toyopearl MX‐Trp‐650M was studied systematically at 25℃. Equilibrium adsorption isotherms at pH 5.0 and 6.0 were measured and compared to experimental data at pH 7.0 from previous work. For all pH values, an exponential decay of the lysozyme loading with increasing ionic strength was observed. The influence of the pH value was found to depend significantly on the ionic strength with the strongest influence at low ionic strengths where increasing pH values lead to decreasing lysozyme loadings. Furthermore, a mathematical model that describes the influence of salts and the pH value on the adsorption of lysozyme in MMC is presented. The model enables predicting adsorption isotherms of lysozyme on Toyopearl MX‐Trp‐650M for a broad range of technically relevant conditions.Jannette KreusserFabian JirasekHans HasseWiley-VCHarticleadsorption isothermschromatographymodelingpHproteinBiotechnologyTP248.13-248.65ENEngineering in Life Sciences, Vol 21, Iss 11, Pp 753-768 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adsorption isotherms chromatography modeling pH protein Biotechnology TP248.13-248.65
spellingShingle	adsorption isotherms chromatography modeling pH protein Biotechnology TP248.13-248.65 Jannette Kreusser Fabian Jirasek Hans Hasse Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography
description	Abstract Mixed‐mode chromatography (MMC) is an interesting technique for challenging protein separation processes which typically combines adsorption mechanisms of ion exchange (IEC) and hydrophobic interaction chromatography (HIC). Adsorption equilibria in MMC depend on multiple parameters but systematic studies on their influence are scarce. In the present work, the influence of the pH value and ionic strengths up to 3000 mM of four technically relevant salts (sodium chloride, sodium sulfate, ammonium chloride, and ammonium sulfate) on the lysozyme adsorption on the mixed‐mode resin Toyopearl MX‐Trp‐650M was studied systematically at 25℃. Equilibrium adsorption isotherms at pH 5.0 and 6.0 were measured and compared to experimental data at pH 7.0 from previous work. For all pH values, an exponential decay of the lysozyme loading with increasing ionic strength was observed. The influence of the pH value was found to depend significantly on the ionic strength with the strongest influence at low ionic strengths where increasing pH values lead to decreasing lysozyme loadings. Furthermore, a mathematical model that describes the influence of salts and the pH value on the adsorption of lysozyme in MMC is presented. The model enables predicting adsorption isotherms of lysozyme on Toyopearl MX‐Trp‐650M for a broad range of technically relevant conditions.
format	article
author	Jannette Kreusser Fabian Jirasek Hans Hasse
author_facet	Jannette Kreusser Fabian Jirasek Hans Hasse
author_sort	Jannette Kreusser
title	Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography
title_short	Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography
title_full	Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography
title_fullStr	Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography
title_full_unstemmed	Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography
title_sort	influence of ph value and salts on the adsorption of lysozyme in mixed‐mode chromatography
publisher	Wiley-VCH
publishDate	2021
url	https://doaj.org/article/b69dddd71cd14aa7a8a0dc0eb7af9ec3
work_keys_str_mv	AT jannettekreusser influenceofphvalueandsaltsontheadsorptionoflysozymeinmixedmodechromatography AT fabianjirasek influenceofphvalueandsaltsontheadsorptionoflysozymeinmixedmodechromatography AT hanshasse influenceofphvalueandsaltsontheadsorptionoflysozymeinmixedmodechromatography
_version_	1718441327373320192

Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography

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