Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone

Abstract Oxidative and glycolytic muscle fibers differ in their ultrastructure, metabolism, and responses to physiological stimuli and pathological insults. We examined whether these fibers respond differentially to exogenous anabolic androgenic steroids (AASs) by comparing morphological and histolo...

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Autores principales:	Hasan A. Asfour, Emad I. Shaqoura, Raed S. Said, Ayman G. Mustafa, Bright Starling Emerald, Mohammed Z. Allouh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6a050f4c351490b9e23096cba67f35d
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spelling	oai:doaj.org-article:b6a050f4c351490b9e23096cba67f35d2021-12-02T14:58:20ZDifferential response of oxidative and glycolytic skeletal muscle fibers to mesterolone10.1038/s41598-021-91854-42045-2322https://doaj.org/article/b6a050f4c351490b9e23096cba67f35d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91854-4https://doaj.org/toc/2045-2322Abstract Oxidative and glycolytic muscle fibers differ in their ultrastructure, metabolism, and responses to physiological stimuli and pathological insults. We examined whether these fibers respond differentially to exogenous anabolic androgenic steroids (AASs) by comparing morphological and histological changes between the oxidative anterior latissimus dorsi (ALD) and glycolytic pectoralis major (PM) fibers in adult avian muscles. Adult female White Leghorn chickens (Gallus gallus) were randomly divided into five groups: a vehicle control and four mesterolone treatment groups (4, 8, 12, and 16 mg/kg). Mesterolone was administered orally every three days for four weeks. Immunocytochemical techniques and morphometric analyses were employed to measure the changes in muscle weight, fiber size, satellite cell (SC) composition, and number of myonuclei. Mesterolone increased both body and muscle weights and induced hypertrophy in glycolytic PM fibers but not in oxidative ALD fibers. Mesterolone induced SC proliferation in both muscles; however, the myonuclear accretion was noticeable only in the PM muscle. In both muscles, the collective changes maintained a constant myonuclear domain size and the changes were dose independent. In conclusion, mesterolone induced distinct dose-independent effects in avian oxidative and glycolytic skeletal muscle fibers; these findings might be clinically valuable in the treatment of age-related sarcopenia.Hasan A. AsfourEmad I. ShaqouraRaed S. SaidAyman G. MustafaBright Starling EmeraldMohammed Z. AllouhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hasan A. Asfour Emad I. Shaqoura Raed S. Said Ayman G. Mustafa Bright Starling Emerald Mohammed Z. Allouh Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone
description	Abstract Oxidative and glycolytic muscle fibers differ in their ultrastructure, metabolism, and responses to physiological stimuli and pathological insults. We examined whether these fibers respond differentially to exogenous anabolic androgenic steroids (AASs) by comparing morphological and histological changes between the oxidative anterior latissimus dorsi (ALD) and glycolytic pectoralis major (PM) fibers in adult avian muscles. Adult female White Leghorn chickens (Gallus gallus) were randomly divided into five groups: a vehicle control and four mesterolone treatment groups (4, 8, 12, and 16 mg/kg). Mesterolone was administered orally every three days for four weeks. Immunocytochemical techniques and morphometric analyses were employed to measure the changes in muscle weight, fiber size, satellite cell (SC) composition, and number of myonuclei. Mesterolone increased both body and muscle weights and induced hypertrophy in glycolytic PM fibers but not in oxidative ALD fibers. Mesterolone induced SC proliferation in both muscles; however, the myonuclear accretion was noticeable only in the PM muscle. In both muscles, the collective changes maintained a constant myonuclear domain size and the changes were dose independent. In conclusion, mesterolone induced distinct dose-independent effects in avian oxidative and glycolytic skeletal muscle fibers; these findings might be clinically valuable in the treatment of age-related sarcopenia.
format	article
author	Hasan A. Asfour Emad I. Shaqoura Raed S. Said Ayman G. Mustafa Bright Starling Emerald Mohammed Z. Allouh
author_facet	Hasan A. Asfour Emad I. Shaqoura Raed S. Said Ayman G. Mustafa Bright Starling Emerald Mohammed Z. Allouh
author_sort	Hasan A. Asfour
title	Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone
title_short	Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone
title_full	Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone
title_fullStr	Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone
title_full_unstemmed	Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone
title_sort	differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b6a050f4c351490b9e23096cba67f35d
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Differential response of oxidative and glycolytic skeletal muscle fibers to mesterolone

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