The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists

Abstract Formaldehyde (FA) is a human carcinogen used as formalin in hospital laboratories. We evaluated its association with human chromosomal aberrations (CAs) and the risk/protective role played by several genetic polymorphisms in this relationship, on a cohort of 57 exposed pathologists vs 48 co...

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Autores principales:	Federica Ghelli, Enrico Cocchi, Martina Buglisi, Giulia Squillacioti, Valeria Bellisario, Roberto Bono, Alfredo Santovito
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6a0ec0dc7884bf88a8ec6a232699725
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spelling	oai:doaj.org-article:b6a0ec0dc7884bf88a8ec6a2326997252021-12-02T15:52:25ZThe role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists10.1038/s41598-021-89833-w2045-2322https://doaj.org/article/b6a0ec0dc7884bf88a8ec6a2326997252021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89833-whttps://doaj.org/toc/2045-2322Abstract Formaldehyde (FA) is a human carcinogen used as formalin in hospital laboratories. We evaluated its association with human chromosomal aberrations (CAs) and the risk/protective role played by several genetic polymorphisms in this relationship, on a cohort of 57 exposed pathologists vs 48 controls. All subjects were assessed for CAs on peripheral blood lymphocytes and genotyped for the most common cancer-associated gene polymorphisms which could be related with the genotoxic outcome: CYP1A1 exon 7 (A>G), CYP1A12A (T>C), CYP2C192 (G>A), GSTT1 (Positive/Null), GSTM1 (Positive/null), GSTP1 (A>G), XRCC1 (G399A), XRCC1 (C194T), XRCC1 (A280G), XPD (A751C), XPC exon 15 (A939C), XPC exon 9 (C499T), TNFα − 308 (G>A), IL10 − 1082 (G>A), IL10 − 819 (C>T) and IL6 − 174 (G>C). Air-FA concentration was assessed through personal samplers. The comparison between pathologists and controls showed a significantly higher CAs frequency in pathologists. Significant positive correlations were found between CAs frequency and air-FA concentration while significant associations were found between variation in CAs frequency and the mutated allele for CYP1A1 exon 7 (A>G), CYP2C19*2 (G>A), GSTT1-positive, GSTM1-positive and XRCC1 (G399A). Our study confirms the role of FA as genotoxicity inductor, even in workers chronically exposed to low air-FA levels and reveals the role played by some genetic polymorphisms in this association, highlighting the importance of individual susceptibility biomarkers assessment in occupational health studies.Federica GhelliEnrico CocchiMartina BuglisiGiulia SquillaciotiValeria BellisarioRoberto BonoAlfredo SantovitoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Federica Ghelli Enrico Cocchi Martina Buglisi Giulia Squillacioti Valeria Bellisario Roberto Bono Alfredo Santovito The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists
description	Abstract Formaldehyde (FA) is a human carcinogen used as formalin in hospital laboratories. We evaluated its association with human chromosomal aberrations (CAs) and the risk/protective role played by several genetic polymorphisms in this relationship, on a cohort of 57 exposed pathologists vs 48 controls. All subjects were assessed for CAs on peripheral blood lymphocytes and genotyped for the most common cancer-associated gene polymorphisms which could be related with the genotoxic outcome: CYP1A1 exon 7 (A>G), CYP1A12A (T>C), CYP2C192 (G>A), GSTT1 (Positive/Null), GSTM1 (Positive/null), GSTP1 (A>G), XRCC1 (G399A), XRCC1 (C194T), XRCC1 (A280G), XPD (A751C), XPC exon 15 (A939C), XPC exon 9 (C499T), TNFα − 308 (G>A), IL10 − 1082 (G>A), IL10 − 819 (C>T) and IL6 − 174 (G>C). Air-FA concentration was assessed through personal samplers. The comparison between pathologists and controls showed a significantly higher CAs frequency in pathologists. Significant positive correlations were found between CAs frequency and air-FA concentration while significant associations were found between variation in CAs frequency and the mutated allele for CYP1A1 exon 7 (A>G), CYP2C19*2 (G>A), GSTT1-positive, GSTM1-positive and XRCC1 (G399A). Our study confirms the role of FA as genotoxicity inductor, even in workers chronically exposed to low air-FA levels and reveals the role played by some genetic polymorphisms in this association, highlighting the importance of individual susceptibility biomarkers assessment in occupational health studies.
format	article
author	Federica Ghelli Enrico Cocchi Martina Buglisi Giulia Squillacioti Valeria Bellisario Roberto Bono Alfredo Santovito
author_facet	Federica Ghelli Enrico Cocchi Martina Buglisi Giulia Squillacioti Valeria Bellisario Roberto Bono Alfredo Santovito
author_sort	Federica Ghelli
title	The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists
title_short	The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists
title_full	The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists
title_fullStr	The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists
title_full_unstemmed	The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists
title_sort	role of phase i, phase ii, and dna-repair gene polymorphisms in the damage induced by formaldehyde in pathologists
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b6a0ec0dc7884bf88a8ec6a232699725
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The role of phase I, phase II, and DNA-repair gene polymorphisms in the damage induced by formaldehyde in pathologists

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