Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer

Abstract Microbial dysbiosis has been implicated in the pathogenesis of oral cancer. We analyzed the compositional and metabolic profile of the bacteriome in three specific niches in oral cancer patients along with controls using 16SrRNA sequencing (Illumina Miseq) and DADA2 software. We found major...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Divya Gopinath, Rohit Kunnath Menon, Chong Chun Wie, Moinak Banerjee, Swagatika Panda, Deviprasad Mandal, Paresh Kumar Behera, Susanta Roychoudhury, Supriya Kheur, Michael George Botelho, Newell W. Johnson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6a789fabe42474790f23d84dd364556
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b6a789fabe42474790f23d84dd364556
record_format	dspace
spelling	oai:doaj.org-article:b6a789fabe42474790f23d84dd3645562021-12-02T14:01:20ZDifferences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer10.1038/s41598-020-80859-02045-2322https://doaj.org/article/b6a789fabe42474790f23d84dd3645562021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80859-0https://doaj.org/toc/2045-2322Abstract Microbial dysbiosis has been implicated in the pathogenesis of oral cancer. We analyzed the compositional and metabolic profile of the bacteriome in three specific niches in oral cancer patients along with controls using 16SrRNA sequencing (Illumina Miseq) and DADA2 software. We found major differences between patients and control subjects. Bacterial communities associated with the tumor surface and deep paired tumor tissue differed significantly. Tumor surfaces carried elevated abundances of taxa belonging to genera Porphyromonas, Enterobacteriae, Neisseria, Streptococcus and Fusobacteria, whereas Prevotella, Treponema, Sphingomonas, Meiothermus and Mycoplasma genera were significantly more abundant in deep tissue. The most abundant microbial metabolic pathways were those related to fatty-acid biosynthesis, carbon metabolism and amino-acid metabolism on the tumor surface: carbohydrate metabolism and organic polymer degradation were elevated in tumor tissues. The bacteriome of saliva from patients with oral cancer differed significantly from paired tumor tissue in terms of community structure, however remained similar at taxonomic and metabolic levels except for elevated abundances of Streptococcus, Lactobacillus and Bacteroides, and acetoin-biosynthesis, respectively. These shifts to a pro-inflammatory profile are consistent with other studies suggesting oncogenic properties. Importantly, selection of the principal source of microbial DNA is key to ensure reliable, reproducible and comparable results in microbiome studies.Divya GopinathRohit Kunnath MenonChong Chun WieMoinak BanerjeeSwagatika PandaDeviprasad MandalParesh Kumar BeheraSusanta RoychoudhurySupriya KheurMichael George BotelhoNewell W. JohnsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Divya Gopinath Rohit Kunnath Menon Chong Chun Wie Moinak Banerjee Swagatika Panda Deviprasad Mandal Paresh Kumar Behera Susanta Roychoudhury Supriya Kheur Michael George Botelho Newell W. Johnson Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
description	Abstract Microbial dysbiosis has been implicated in the pathogenesis of oral cancer. We analyzed the compositional and metabolic profile of the bacteriome in three specific niches in oral cancer patients along with controls using 16SrRNA sequencing (Illumina Miseq) and DADA2 software. We found major differences between patients and control subjects. Bacterial communities associated with the tumor surface and deep paired tumor tissue differed significantly. Tumor surfaces carried elevated abundances of taxa belonging to genera Porphyromonas, Enterobacteriae, Neisseria, Streptococcus and Fusobacteria, whereas Prevotella, Treponema, Sphingomonas, Meiothermus and Mycoplasma genera were significantly more abundant in deep tissue. The most abundant microbial metabolic pathways were those related to fatty-acid biosynthesis, carbon metabolism and amino-acid metabolism on the tumor surface: carbohydrate metabolism and organic polymer degradation were elevated in tumor tissues. The bacteriome of saliva from patients with oral cancer differed significantly from paired tumor tissue in terms of community structure, however remained similar at taxonomic and metabolic levels except for elevated abundances of Streptococcus, Lactobacillus and Bacteroides, and acetoin-biosynthesis, respectively. These shifts to a pro-inflammatory profile are consistent with other studies suggesting oncogenic properties. Importantly, selection of the principal source of microbial DNA is key to ensure reliable, reproducible and comparable results in microbiome studies.
format	article
author	Divya Gopinath Rohit Kunnath Menon Chong Chun Wie Moinak Banerjee Swagatika Panda Deviprasad Mandal Paresh Kumar Behera Susanta Roychoudhury Supriya Kheur Michael George Botelho Newell W. Johnson
author_facet	Divya Gopinath Rohit Kunnath Menon Chong Chun Wie Moinak Banerjee Swagatika Panda Deviprasad Mandal Paresh Kumar Behera Susanta Roychoudhury Supriya Kheur Michael George Botelho Newell W. Johnson
author_sort	Divya Gopinath
title	Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
title_short	Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
title_full	Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
title_fullStr	Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
title_full_unstemmed	Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
title_sort	differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b6a789fabe42474790f23d84dd364556
work_keys_str_mv	AT divyagopinath differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT rohitkunnathmenon differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT chongchunwie differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT moinakbanerjee differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT swagatikapanda differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT deviprasadmandal differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT pareshkumarbehera differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT susantaroychoudhury differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT supriyakheur differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT michaelgeorgebotelho differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer AT newellwjohnson differencesinthebacteriomeofswabsalivaandtissuebiopsiesinoralcancer
_version_	1718392223031099392

Differences in the bacteriome of swab, saliva, and tissue biopsies in oral cancer

Ejemplares similares