Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance

Objective To investigate the role of PKCι, YAP1 and high-risk HPV infection in the local immune microenvironment of cervical cancer. Methods We chose 80 cases of normal tissue of the cervix (NCT), cervical low-grade squamous intraepithelial lesion (LSIL), cervical high-grade squamous intraepithelial...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	CHEN Shengmin, WANG Guoping, LIN Zhiren, YUAN Feng
Formato:	article
Lenguaje:	ZH
Publicado:	Magazine House of Cancer Research on Prevention and Treatment 2021
Materias:	cervical carcinoma hpv protein kinase cι yes-associated protein 1 immune microenviroment Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/b6b80ccdf23c409d800f6afbd415d84a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b6b80ccdf23c409d800f6afbd415d84a
record_format	dspace
spelling	oai:doaj.org-article:b6b80ccdf23c409d800f6afbd415d84a2021-11-05T02:22:52ZExpression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance1000-857810.3971/j.issn.1000-8578.2021.21.0340https://doaj.org/article/b6b80ccdf23c409d800f6afbd415d84a2021-10-01T00:00:00Zhttp://html.rhhz.net/ZLFZYJ/html/8578.2021.21.0340.htmhttps://doaj.org/toc/1000-8578Objective To investigate the role of PKCι, YAP1 and high-risk HPV infection in the local immune microenvironment of cervical cancer. Methods We chose 80 cases of normal tissue of the cervix (NCT), cervical low-grade squamous intraepithelial lesion (LSIL), cervical high-grade squamous intraepithelial lesion (HSIL) and early cervical squamous cell carcinoma (SCC) each. Four groups were collected.The infection rate of high-risk HPV in four groups was determined by real-time fluorescence PCR method. The expression levels of PKCι, YAP1, CD4 and CD8 in four groups were measured and correlated by IHC and clinicopathologic features were also analyzed. Results The differences of high-risk HPV infection rate and PKCι, YAP1, CD4, CD8 positive rate among groups of NCT, LSIL, HSIL and SCC had statistical significance (P < 0.05). The level of cervical lesions was positively associated with high-risk HPV infection and positive PKCι, YAP1, CD8 expression (P < 0.05), while negatively associated with positive CD4 expression (P < 0.05). HPV infection and positive PKCι, YAP1, CD8 expression were positively correlated with each other in SCC, while were all negatively correlated with positive CD4 expression(P < 0.05). The differences of HPV infection, PKCι, YAP1 and CD8 positive expression were significant in different levels of differentiation and vascular invasion of SCC (P < 0.05). Conclusion The patients with cervical lesions are often accompanied by high-risk HPV infection and abnormal expression of PKCι, YAP1, CD4 and CD8, which may have synergistic effects on each other, causing the local immunosuppression microenvironment of SCC. It provides a possible strategy for the study of pathogenesis, diagnosis and treatment of cervical cancer.CHEN ShengminWANG GuopingLIN ZhirenYUAN FengMagazine House of Cancer Research on Prevention and Treatmentarticlecervical carcinomahpvprotein kinase cιyes-associated protein 1immune microenviromentNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ZHZhongliu Fangzhi Yanjiu, Vol 48, Iss 10, Pp 970-973 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	cervical carcinoma hpv protein kinase cι yes-associated protein 1 immune microenviroment Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	cervical carcinoma hpv protein kinase cι yes-associated protein 1 immune microenviroment Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 CHEN Shengmin WANG Guoping LIN Zhiren YUAN Feng Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance
description	Objective To investigate the role of PKCι, YAP1 and high-risk HPV infection in the local immune microenvironment of cervical cancer. Methods We chose 80 cases of normal tissue of the cervix (NCT), cervical low-grade squamous intraepithelial lesion (LSIL), cervical high-grade squamous intraepithelial lesion (HSIL) and early cervical squamous cell carcinoma (SCC) each. Four groups were collected.The infection rate of high-risk HPV in four groups was determined by real-time fluorescence PCR method. The expression levels of PKCι, YAP1, CD4 and CD8 in four groups were measured and correlated by IHC and clinicopathologic features were also analyzed. Results The differences of high-risk HPV infection rate and PKCι, YAP1, CD4, CD8 positive rate among groups of NCT, LSIL, HSIL and SCC had statistical significance (P < 0.05). The level of cervical lesions was positively associated with high-risk HPV infection and positive PKCι, YAP1, CD8 expression (P < 0.05), while negatively associated with positive CD4 expression (P < 0.05). HPV infection and positive PKCι, YAP1, CD8 expression were positively correlated with each other in SCC, while were all negatively correlated with positive CD4 expression(P < 0.05). The differences of HPV infection, PKCι, YAP1 and CD8 positive expression were significant in different levels of differentiation and vascular invasion of SCC (P < 0.05). Conclusion The patients with cervical lesions are often accompanied by high-risk HPV infection and abnormal expression of PKCι, YAP1, CD4 and CD8, which may have synergistic effects on each other, causing the local immunosuppression microenvironment of SCC. It provides a possible strategy for the study of pathogenesis, diagnosis and treatment of cervical cancer.
format	article
author	CHEN Shengmin WANG Guoping LIN Zhiren YUAN Feng
author_facet	CHEN Shengmin WANG Guoping LIN Zhiren YUAN Feng
author_sort	CHEN Shengmin
title	Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance
title_short	Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance
title_full	Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance
title_fullStr	Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance
title_full_unstemmed	Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance
title_sort	expression of pkcι/yap1 in cervical cancer and its clinical significance
publisher	Magazine House of Cancer Research on Prevention and Treatment
publishDate	2021
url	https://doaj.org/article/b6b80ccdf23c409d800f6afbd415d84a
work_keys_str_mv	AT chenshengmin expressionofpkciyap1incervicalcanceranditsclinicalsignificance AT wangguoping expressionofpkciyap1incervicalcanceranditsclinicalsignificance AT linzhiren expressionofpkciyap1incervicalcanceranditsclinicalsignificance AT yuanfeng expressionofpkciyap1incervicalcanceranditsclinicalsignificance
_version_	1718444550858473472

Expression of PKCι/YAP1 in Cervical Cancer and Its Clinical Significance

Ejemplares similares