Age and diet affect gene expression profile in canine skeletal muscle.

We evaluated gene transcription in canine skeletal muscle (biceps femoris) using microarray analysis to identify effects of age and diet on gene expression. Twelve female beagles were used (six 1-year olds and six 12-year olds) and they were fed one of two experimental diets for 12 months. One diet...

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Autores principales:	Ingmar S Middelbos, Brittany M Vester, Lisa K Karr-Lilienthal, Lawrence B Schook, Kelly S Swanson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6c028dd75614838ab4b01c7b8c16e07
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spelling	oai:doaj.org-article:b6c028dd75614838ab4b01c7b8c16e072021-11-25T06:17:16ZAge and diet affect gene expression profile in canine skeletal muscle.1932-620310.1371/journal.pone.0004481https://doaj.org/article/b6c028dd75614838ab4b01c7b8c16e072009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19221602/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We evaluated gene transcription in canine skeletal muscle (biceps femoris) using microarray analysis to identify effects of age and diet on gene expression. Twelve female beagles were used (six 1-year olds and six 12-year olds) and they were fed one of two experimental diets for 12 months. One diet contained primarily plant-based protein sources (PPB), whereas the second diet contained primarily animal-based protein sources (APB). Affymetrix GeneChip Canine Genome Arrays were used to hybridize extracted RNA. Age had the greatest effect on gene transcription (262 differentially expressed genes), whereas the effect of diet was relatively small (22 differentially expressed genes). Effects of age (regardless of diet) were most notable on genes related to metabolism, cell cycle and cell development, and transcription function. All these genes were predominantly down-regulated in geriatric dogs. Age-affected genes that were differentially expressed on only one of two diets were primarily noted in the PPB diet group (144/165 genes). Again, genes related to cell cycle (22/35) and metabolism (15/19) had predominantly decreased transcription in geriatric dogs, but 6/8 genes related to muscle development had increased expression. Effects of diet on muscle gene expression were mostly noted in geriatric dogs, but no consistent patterns in transcription were observed. The insight these data provide into gene expression profiles of canine skeletal muscle as affected by age, could serve as a foundation for future research pertaining to age-related muscle diseases.Ingmar S MiddelbosBrittany M VesterLisa K Karr-LilienthalLawrence B SchookKelly S SwansonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 2, p e4481 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ingmar S Middelbos Brittany M Vester Lisa K Karr-Lilienthal Lawrence B Schook Kelly S Swanson Age and diet affect gene expression profile in canine skeletal muscle.
description	We evaluated gene transcription in canine skeletal muscle (biceps femoris) using microarray analysis to identify effects of age and diet on gene expression. Twelve female beagles were used (six 1-year olds and six 12-year olds) and they were fed one of two experimental diets for 12 months. One diet contained primarily plant-based protein sources (PPB), whereas the second diet contained primarily animal-based protein sources (APB). Affymetrix GeneChip Canine Genome Arrays were used to hybridize extracted RNA. Age had the greatest effect on gene transcription (262 differentially expressed genes), whereas the effect of diet was relatively small (22 differentially expressed genes). Effects of age (regardless of diet) were most notable on genes related to metabolism, cell cycle and cell development, and transcription function. All these genes were predominantly down-regulated in geriatric dogs. Age-affected genes that were differentially expressed on only one of two diets were primarily noted in the PPB diet group (144/165 genes). Again, genes related to cell cycle (22/35) and metabolism (15/19) had predominantly decreased transcription in geriatric dogs, but 6/8 genes related to muscle development had increased expression. Effects of diet on muscle gene expression were mostly noted in geriatric dogs, but no consistent patterns in transcription were observed. The insight these data provide into gene expression profiles of canine skeletal muscle as affected by age, could serve as a foundation for future research pertaining to age-related muscle diseases.
format	article
author	Ingmar S Middelbos Brittany M Vester Lisa K Karr-Lilienthal Lawrence B Schook Kelly S Swanson
author_facet	Ingmar S Middelbos Brittany M Vester Lisa K Karr-Lilienthal Lawrence B Schook Kelly S Swanson
author_sort	Ingmar S Middelbos
title	Age and diet affect gene expression profile in canine skeletal muscle.
title_short	Age and diet affect gene expression profile in canine skeletal muscle.
title_full	Age and diet affect gene expression profile in canine skeletal muscle.
title_fullStr	Age and diet affect gene expression profile in canine skeletal muscle.
title_full_unstemmed	Age and diet affect gene expression profile in canine skeletal muscle.
title_sort	age and diet affect gene expression profile in canine skeletal muscle.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/b6c028dd75614838ab4b01c7b8c16e07
work_keys_str_mv	AT ingmarsmiddelbos ageanddietaffectgeneexpressionprofileincanineskeletalmuscle AT brittanymvester ageanddietaffectgeneexpressionprofileincanineskeletalmuscle AT lisakkarrlilienthal ageanddietaffectgeneexpressionprofileincanineskeletalmuscle AT lawrencebschook ageanddietaffectgeneexpressionprofileincanineskeletalmuscle AT kellysswanson ageanddietaffectgeneexpressionprofileincanineskeletalmuscle
_version_	1718414004881195008

Age and diet affect gene expression profile in canine skeletal muscle.

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