Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
Abstract SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests...
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Nature Portfolio
2021
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oai:doaj.org-article:b6c6d37fd4a044d496e9fc4b7cf56f4f2021-12-02T15:45:21ZFlow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection10.1038/s41598-021-90054-42045-2322https://doaj.org/article/b6c6d37fd4a044d496e9fc4b7cf56f4f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90054-4https://doaj.org/toc/2045-2322Abstract SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection.Paula PiñeroFrancisco M Marco De La CalleLydia HorndlerBalbino AlarcónMarisol Uribe BarrientosHéctor SarmientoFabián TarínNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-5 (2021) |
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Medicine R Science Q Paula Piñero Francisco M Marco De La Calle Lydia Horndler Balbino Alarcón Marisol Uribe Barrientos Héctor Sarmiento Fabián Tarín Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection |
| description |
Abstract SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection. |
| format |
article |
| author |
Paula Piñero Francisco M Marco De La Calle Lydia Horndler Balbino Alarcón Marisol Uribe Barrientos Héctor Sarmiento Fabián Tarín |
| author_facet |
Paula Piñero Francisco M Marco De La Calle Lydia Horndler Balbino Alarcón Marisol Uribe Barrientos Héctor Sarmiento Fabián Tarín |
| author_sort |
Paula Piñero |
| title |
Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection |
| title_short |
Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection |
| title_full |
Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection |
| title_fullStr |
Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection |
| title_full_unstemmed |
Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection |
| title_sort |
flow cytometry detection of sustained humoral immune response (igg + iga) against native spike glycoprotein in asymptomatic/mild sars-cov-2 infection |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/b6c6d37fd4a044d496e9fc4b7cf56f4f |
| work_keys_str_mv |
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