Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers

Tree peony has nine wild species, but the evolutionary relationship of them is still unclear. Here, a total of 274 specimens from 22 natural populations of nine wild species were collected, and their genetic diversity and similarity was analyzed based on Simple Sequence Repeats (SSR) molecular marke...

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Autores principales:	Yuqian Xue, Rong Liu, Jingqi Xue, Shunli Wang, Xiuxin Zhang
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Tree peony Molecular marker Genetic diversity Phylogenetic relationship Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/b6c7144baa0b46d0a5475a2e9bd116b7
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spelling	oai:doaj.org-article:b6c7144baa0b46d0a5475a2e9bd116b72021-11-12T04:43:26ZGenetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers2468-014110.1016/j.hpj.2021.05.004https://doaj.org/article/b6c7144baa0b46d0a5475a2e9bd116b72021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S246801412100073Xhttps://doaj.org/toc/2468-0141Tree peony has nine wild species, but the evolutionary relationship of them is still unclear. Here, a total of 274 specimens from 22 natural populations of nine wild species were collected, and their genetic diversity and similarity was analyzed based on Simple Sequence Repeats (SSR) molecular markers. A total of 106 alleles were generated based on 20 primers and with an average of 5.3 alleles per primer. Shannon's information index (I) ranged from 0.6333 to 1.7842, and the average of Nei's genetic diversity coefficient (H) was 0.5771. Polymorphism Information Content (PIC) value varied from 0.29 to 0.77, ten of these primers had high polymorphism (PIC ≥ 0.50). All the above genetic parameters of primers reflect more rich genetic diversity information compared with other researches using SSR molecular markers to study the genetic diversity of tree peony wild species. At the population level, the lowest and highest degree of genetic diversity occurred in Paeonia ludlowii-P1 and P. delavayi-P3 population, respectively. Whereas at species level, the genetic diversity of 9 wild peony species was as follows: P. lutea > P. delavayi > P. rockii > P. qiui > P. ostii > P. decomposita > P. potaninii > P. spontanea > P. ludlowii. Furthermore, cluster analysis at species level divided the nine wild tree peony species into two branches. In branch I, the closest phylogenetic relationship was found between P. ostii and P. rockii, followed by P. spontanea, P. qiui, and P. decomposita. In branch II, the closest relationship occurred between P. lutea and P. delavayi, followed by P. potaninii and P. ludlowii. Clustering results supported the division of tree peonies into two subsects (Delavayanae and Vaginatae), it also supported P. potaninii and P. ludlowii as independent species. The results provided novel insight into the genetic diversity and phylogenetic relationship of nine wild tree peony species. It will help formulate comprehensive protection measures of wild germplasm resources and select proper parents for distant hybridization in the future.Yuqian XueRong LiuJingqi XueShunli WangXiuxin ZhangKeAi Communications Co., Ltd.articleTree peonyMolecular markerGenetic diversityPhylogenetic relationshipPlant cultureSB1-1110ENHorticultural Plant Journal, Vol 7, Iss 6, Pp 579-588 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tree peony Molecular marker Genetic diversity Phylogenetic relationship Plant culture SB1-1110
spellingShingle	Tree peony Molecular marker Genetic diversity Phylogenetic relationship Plant culture SB1-1110 Yuqian Xue Rong Liu Jingqi Xue Shunli Wang Xiuxin Zhang Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers
description	Tree peony has nine wild species, but the evolutionary relationship of them is still unclear. Here, a total of 274 specimens from 22 natural populations of nine wild species were collected, and their genetic diversity and similarity was analyzed based on Simple Sequence Repeats (SSR) molecular markers. A total of 106 alleles were generated based on 20 primers and with an average of 5.3 alleles per primer. Shannon's information index (I) ranged from 0.6333 to 1.7842, and the average of Nei's genetic diversity coefficient (H) was 0.5771. Polymorphism Information Content (PIC) value varied from 0.29 to 0.77, ten of these primers had high polymorphism (PIC ≥ 0.50). All the above genetic parameters of primers reflect more rich genetic diversity information compared with other researches using SSR molecular markers to study the genetic diversity of tree peony wild species. At the population level, the lowest and highest degree of genetic diversity occurred in Paeonia ludlowii-P1 and P. delavayi-P3 population, respectively. Whereas at species level, the genetic diversity of 9 wild peony species was as follows: P. lutea > P. delavayi > P. rockii > P. qiui > P. ostii > P. decomposita > P. potaninii > P. spontanea > P. ludlowii. Furthermore, cluster analysis at species level divided the nine wild tree peony species into two branches. In branch I, the closest phylogenetic relationship was found between P. ostii and P. rockii, followed by P. spontanea, P. qiui, and P. decomposita. In branch II, the closest relationship occurred between P. lutea and P. delavayi, followed by P. potaninii and P. ludlowii. Clustering results supported the division of tree peonies into two subsects (Delavayanae and Vaginatae), it also supported P. potaninii and P. ludlowii as independent species. The results provided novel insight into the genetic diversity and phylogenetic relationship of nine wild tree peony species. It will help formulate comprehensive protection measures of wild germplasm resources and select proper parents for distant hybridization in the future.
format	article
author	Yuqian Xue Rong Liu Jingqi Xue Shunli Wang Xiuxin Zhang
author_facet	Yuqian Xue Rong Liu Jingqi Xue Shunli Wang Xiuxin Zhang
author_sort	Yuqian Xue
title	Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers
title_short	Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers
title_full	Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers
title_fullStr	Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers
title_full_unstemmed	Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers
title_sort	genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/b6c7144baa0b46d0a5475a2e9bd116b7
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_version_	1718431254001483776

Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers

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