Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.

Conidia of Aspergillus fumigatus are inhaled by humans on daily basis. As a consequence, these conidia can cause infections that differ in severity ranging from allergic bronchopulmonary aspergillosis to invasive aspergillosis. In this study we compared virulence of five A. fumigatus isolates in fou...

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Autores principales:	E M Keizer, I D Valdes, G Forn-Cuni, E Klijn, A H Meijer, F Hillman, H A B Wösten, H de Cock
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6d24c3ee6d74915adfa54ab650364d4
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spelling	oai:doaj.org-article:b6d24c3ee6d74915adfa54ab650364d42021-12-02T20:05:06ZVariation of virulence of five Aspergillus fumigatus isolates in four different infection models.1932-620310.1371/journal.pone.0252948https://doaj.org/article/b6d24c3ee6d74915adfa54ab650364d42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252948https://doaj.org/toc/1932-6203Conidia of Aspergillus fumigatus are inhaled by humans on daily basis. As a consequence, these conidia can cause infections that differ in severity ranging from allergic bronchopulmonary aspergillosis to invasive aspergillosis. In this study we compared virulence of five A. fumigatus isolates in four different infection models to address the predictive value of different model systems. Two of the A. fumigatus strains were isolated from dogs with a non-invasive sino-nasal aspergillosis (DTO271-B5 and DTO303-F3), while three strains were isolated from human patients with invasive aspergillosis (Af293, ATCC46645 and CEA10). Infection models used encompassed cultured type II A549 lung epithelial cells, Protostelium aurantium amoeba, Galleria melonella larvae and zebrafish embryos. No major differences in virulence between these five strains were observed in the lung epithelial cell model. In contrast, strain ATCC46645 was most virulent in the amoeba and zebrafish model, whereas it was much less virulent in the Galleria infection model. DTO303-F3 was most virulent in the latter model. In general, reference strain Af293 was less virulent as compared to the other strains. Genome sequence analysis showed that this latter strain differed from the other four strains in 136 SNPs in virulence-related genes. Together, our results show that virulence of individual A. fumigatus strains show significant differences between infection models. We conclude that the predictive value of different model systems varies since the relative virulence across fungal strains does not hold up across different infection model systems.E M KeizerI D ValdesG Forn-CuniE KlijnA H MeijerF HillmanH A B WöstenH de CockPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0252948 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E M Keizer I D Valdes G Forn-Cuni E Klijn A H Meijer F Hillman H A B Wösten H de Cock Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.
description	Conidia of Aspergillus fumigatus are inhaled by humans on daily basis. As a consequence, these conidia can cause infections that differ in severity ranging from allergic bronchopulmonary aspergillosis to invasive aspergillosis. In this study we compared virulence of five A. fumigatus isolates in four different infection models to address the predictive value of different model systems. Two of the A. fumigatus strains were isolated from dogs with a non-invasive sino-nasal aspergillosis (DTO271-B5 and DTO303-F3), while three strains were isolated from human patients with invasive aspergillosis (Af293, ATCC46645 and CEA10). Infection models used encompassed cultured type II A549 lung epithelial cells, Protostelium aurantium amoeba, Galleria melonella larvae and zebrafish embryos. No major differences in virulence between these five strains were observed in the lung epithelial cell model. In contrast, strain ATCC46645 was most virulent in the amoeba and zebrafish model, whereas it was much less virulent in the Galleria infection model. DTO303-F3 was most virulent in the latter model. In general, reference strain Af293 was less virulent as compared to the other strains. Genome sequence analysis showed that this latter strain differed from the other four strains in 136 SNPs in virulence-related genes. Together, our results show that virulence of individual A. fumigatus strains show significant differences between infection models. We conclude that the predictive value of different model systems varies since the relative virulence across fungal strains does not hold up across different infection model systems.
format	article
author	E M Keizer I D Valdes G Forn-Cuni E Klijn A H Meijer F Hillman H A B Wösten H de Cock
author_facet	E M Keizer I D Valdes G Forn-Cuni E Klijn A H Meijer F Hillman H A B Wösten H de Cock
author_sort	E M Keizer
title	Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.
title_short	Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.
title_full	Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.
title_fullStr	Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.
title_full_unstemmed	Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.
title_sort	variation of virulence of five aspergillus fumigatus isolates in four different infection models.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b6d24c3ee6d74915adfa54ab650364d4
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Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.

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