Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.

The rapid and massive degeneration of photoreceptors in retinal degeneration might have a dramatic negative effect on retinal circuits downstream of photoreceptors. However, the impact of photoreceptor loss on the morphology and function of retinal ganglion cells (RGCs) is not fully understood, prec...

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Autores principales:	Bin Lin, Edward Bo Peng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6db6058e6e14e389e5679f81abe6812
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spelling	oai:doaj.org-article:b6db6058e6e14e389e5679f81abe68122021-11-18T07:39:21ZRetinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.1932-620310.1371/journal.pone.0068084https://doaj.org/article/b6db6058e6e14e389e5679f81abe68122013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840814/?tool=EBIhttps://doaj.org/toc/1932-6203The rapid and massive degeneration of photoreceptors in retinal degeneration might have a dramatic negative effect on retinal circuits downstream of photoreceptors. However, the impact of photoreceptor loss on the morphology and function of retinal ganglion cells (RGCs) is not fully understood, precluding the rational design of therapeutic interventions that can reverse the progressive loss of retinal function. The present study investigated the morphological changes in several identified RGCs in the retinal degeneration rd1 mouse model of retinitis pigmentosa (RP), using a combination of viral transfection, microinjection of neurobiotin and confocal microscopy. Individual RGCs were visualized with a high degree of detail using an adeno-associated virus (AAV) vector carrying the gene for enhanced green fluorescent protein (EGFP), allowed for large-scale surveys of the morphology of RGCs over a wide age range. Interestingly, we found that the RGCs of nine different types we encountered were especially resistant to photoreceptor degeneration, and retained their fine dendritic geometry well beyond the complete death of photoreceptors. In addition, the RGC-specific markers revealed a remarkable degree of stability in both morphology and numbers of two identified types of RGCs for up to 18 months of age. Collectively, our data suggest that ganglion cells, the only output cells of the retina, are well preserved morphologically, indicating the ganglion cell population might be an attractive target for treating vision loss.Bin LinEdward Bo PengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e68084 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bin Lin Edward Bo Peng Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.
description	The rapid and massive degeneration of photoreceptors in retinal degeneration might have a dramatic negative effect on retinal circuits downstream of photoreceptors. However, the impact of photoreceptor loss on the morphology and function of retinal ganglion cells (RGCs) is not fully understood, precluding the rational design of therapeutic interventions that can reverse the progressive loss of retinal function. The present study investigated the morphological changes in several identified RGCs in the retinal degeneration rd1 mouse model of retinitis pigmentosa (RP), using a combination of viral transfection, microinjection of neurobiotin and confocal microscopy. Individual RGCs were visualized with a high degree of detail using an adeno-associated virus (AAV) vector carrying the gene for enhanced green fluorescent protein (EGFP), allowed for large-scale surveys of the morphology of RGCs over a wide age range. Interestingly, we found that the RGCs of nine different types we encountered were especially resistant to photoreceptor degeneration, and retained their fine dendritic geometry well beyond the complete death of photoreceptors. In addition, the RGC-specific markers revealed a remarkable degree of stability in both morphology and numbers of two identified types of RGCs for up to 18 months of age. Collectively, our data suggest that ganglion cells, the only output cells of the retina, are well preserved morphologically, indicating the ganglion cell population might be an attractive target for treating vision loss.
format	article
author	Bin Lin Edward Bo Peng
author_facet	Bin Lin Edward Bo Peng
author_sort	Bin Lin
title	Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.
title_short	Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.
title_full	Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.
title_fullStr	Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.
title_full_unstemmed	Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.
title_sort	retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b6db6058e6e14e389e5679f81abe6812
work_keys_str_mv	AT binlin retinalganglioncellsareresistanttophotoreceptorlossinretinaldegeneration AT edwardbopeng retinalganglioncellsareresistanttophotoreceptorlossinretinaldegeneration
_version_	1718423186064801792

Retinal ganglion cells are resistant to photoreceptor loss in retinal degeneration.

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