High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples

Acoustic ejection mass spectrometry is a novel high-throughput analytical technology that delivers high reproducibility without carryover observed. It eliminates the chromatography step used to separate analytes from matrix components. Fully-automated liquid–liquid extraction is widely used for samp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuzhu Guo, Michael Forbush, Thomas R. Covey, Lucien Ghislain, Chang Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	acoustic ejection mass spectrometry high-throughput analysis sample preparation liquid–liquid extraction Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b6f291e4dcb24e2aa3047370fceb9c1c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b6f291e4dcb24e2aa3047370fceb9c1c
record_format	dspace
spelling	oai:doaj.org-article:b6f291e4dcb24e2aa3047370fceb9c1c2021-11-25T18:20:56ZHigh-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples10.3390/metabo111107892218-1989https://doaj.org/article/b6f291e4dcb24e2aa3047370fceb9c1c2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/789https://doaj.org/toc/2218-1989Acoustic ejection mass spectrometry is a novel high-throughput analytical technology that delivers high reproducibility without carryover observed. It eliminates the chromatography step used to separate analytes from matrix components. Fully-automated liquid–liquid extraction is widely used for sample cleanup, especially in high-throughput applications. We introduce a workflow for direct AEMS analysis from phase-separated liquid samples and explore high-throughput analysis from complex matrices. We demonstrate the quantitative determination of fentanyl from urine using this two-phase AEMS approach, with a LOD lower than 1 ng/mL, quantitation precision of 15%, and accuracy better than ±10% over the range of evaluation (1–100 ng/mL). This workflow offers simplified sample preparation and higher analytical throughput for some bioanalytical applications, in comparison to an LC-MS based approach.Yuzhu GuoMichael ForbushThomas R. CoveyLucien GhislainChang LiuMDPI AGarticleacoustic ejection mass spectrometryhigh-throughput analysissample preparationliquid–liquid extractionMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 789, p 789 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acoustic ejection mass spectrometry high-throughput analysis sample preparation liquid–liquid extraction Microbiology QR1-502
spellingShingle	acoustic ejection mass spectrometry high-throughput analysis sample preparation liquid–liquid extraction Microbiology QR1-502 Yuzhu Guo Michael Forbush Thomas R. Covey Lucien Ghislain Chang Liu High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples
description	Acoustic ejection mass spectrometry is a novel high-throughput analytical technology that delivers high reproducibility without carryover observed. It eliminates the chromatography step used to separate analytes from matrix components. Fully-automated liquid–liquid extraction is widely used for sample cleanup, especially in high-throughput applications. We introduce a workflow for direct AEMS analysis from phase-separated liquid samples and explore high-throughput analysis from complex matrices. We demonstrate the quantitative determination of fentanyl from urine using this two-phase AEMS approach, with a LOD lower than 1 ng/mL, quantitation precision of 15%, and accuracy better than ±10% over the range of evaluation (1–100 ng/mL). This workflow offers simplified sample preparation and higher analytical throughput for some bioanalytical applications, in comparison to an LC-MS based approach.
format	article
author	Yuzhu Guo Michael Forbush Thomas R. Covey Lucien Ghislain Chang Liu
author_facet	Yuzhu Guo Michael Forbush Thomas R. Covey Lucien Ghislain Chang Liu
author_sort	Yuzhu Guo
title	High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples
title_short	High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples
title_full	High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples
title_fullStr	High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples
title_full_unstemmed	High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples
title_sort	high-throughput analysis from complex matrices: acoustic ejection mass spectrometry from phase-separated fluid samples
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b6f291e4dcb24e2aa3047370fceb9c1c
work_keys_str_mv	AT yuzhuguo highthroughputanalysisfromcomplexmatricesacousticejectionmassspectrometryfromphaseseparatedfluidsamples AT michaelforbush highthroughputanalysisfromcomplexmatricesacousticejectionmassspectrometryfromphaseseparatedfluidsamples AT thomasrcovey highthroughputanalysisfromcomplexmatricesacousticejectionmassspectrometryfromphaseseparatedfluidsamples AT lucienghislain highthroughputanalysisfromcomplexmatricesacousticejectionmassspectrometryfromphaseseparatedfluidsamples AT changliu highthroughputanalysisfromcomplexmatricesacousticejectionmassspectrometryfromphaseseparatedfluidsamples
_version_	1718411341654392832

High-Throughput Analysis from Complex Matrices: Acoustic Ejection Mass Spectrometry from Phase-Separated Fluid Samples

Ejemplares similares