Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance

Thioglycoligases have proved useful for bonding carbohydrates to non-sugar acceptors, however, the scope of these biocatalysts is usually limited. Here, the authors engineer a xylosidase into a thioglycoligase with the ability to form O-, N-, S- and Se- glycosides together with sugar esters and phos...

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Autores principales:	Manuel Nieto-Domínguez, Beatriz Fernández de Toro, Laura I. de Eugenio, Andrés G. Santana, Lara Bejarano-Muñoz, Zach Armstrong, Juan Antonio Méndez-Líter, Juan Luis Asensio, Alicia Prieto, Stephen G. Withers, Francisco Javier Cañada, María Jesús Martínez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b6f5a98554ec4b30ad3593c389e9d6e2
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spelling	oai:doaj.org-article:b6f5a98554ec4b30ad3593c389e9d6e22021-12-02T18:48:25ZThioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance10.1038/s41467-020-18667-32041-1723https://doaj.org/article/b6f5a98554ec4b30ad3593c389e9d6e22020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18667-3https://doaj.org/toc/2041-1723Thioglycoligases have proved useful for bonding carbohydrates to non-sugar acceptors, however, the scope of these biocatalysts is usually limited. Here, the authors engineer a xylosidase into a thioglycoligase with the ability to form O-, N-, S- and Se- glycosides together with sugar esters and phosphoesters.Manuel Nieto-DomínguezBeatriz Fernández de ToroLaura I. de EugenioAndrés G. SantanaLara Bejarano-MuñozZach ArmstrongJuan Antonio Méndez-LíterJuan Luis AsensioAlicia PrietoStephen G. WithersFrancisco Javier CañadaMaría Jesús MartínezNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Manuel Nieto-Domínguez Beatriz Fernández de Toro Laura I. de Eugenio Andrés G. Santana Lara Bejarano-Muñoz Zach Armstrong Juan Antonio Méndez-Líter Juan Luis Asensio Alicia Prieto Stephen G. Withers Francisco Javier Cañada María Jesús Martínez Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
description	Thioglycoligases have proved useful for bonding carbohydrates to non-sugar acceptors, however, the scope of these biocatalysts is usually limited. Here, the authors engineer a xylosidase into a thioglycoligase with the ability to form O-, N-, S- and Se- glycosides together with sugar esters and phosphoesters.
format	article
author	Manuel Nieto-Domínguez Beatriz Fernández de Toro Laura I. de Eugenio Andrés G. Santana Lara Bejarano-Muñoz Zach Armstrong Juan Antonio Méndez-Líter Juan Luis Asensio Alicia Prieto Stephen G. Withers Francisco Javier Cañada María Jesús Martínez
author_facet	Manuel Nieto-Domínguez Beatriz Fernández de Toro Laura I. de Eugenio Andrés G. Santana Lara Bejarano-Muñoz Zach Armstrong Juan Antonio Méndez-Líter Juan Luis Asensio Alicia Prieto Stephen G. Withers Francisco Javier Cañada María Jesús Martínez
author_sort	Manuel Nieto-Domínguez
title	Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
title_short	Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
title_full	Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
title_fullStr	Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
title_full_unstemmed	Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
title_sort	thioglycoligase derived from fungal gh3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b6f5a98554ec4b30ad3593c389e9d6e2
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Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance

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