Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85

Abstract Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in contin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chia-wei Wu, Thomas Spike, Dawn M. Klingeman, Miguel Rodriguez, Virgil R. Bremer, Steven D. Brown
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6f86f4e710a42288a69e44db6e8bb98
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b6f86f4e710a42288a69e44db6e8bb98
record_format	dspace
spelling	oai:doaj.org-article:b6f86f4e710a42288a69e44db6e8bb982021-12-02T15:05:48ZGeneration and Characterization of Acid Tolerant Fibrobacter succinogenes S8510.1038/s41598-017-02628-w2045-2322https://doaj.org/article/b6f86f4e710a42288a69e44db6e8bb982017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02628-whttps://doaj.org/toc/2045-2322Abstract Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60–5.65, which is lower than has been reported previously. Wild type and acid tolerant strains digested corn stover with equal efficiency in batch culture at low pH. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.10 and 5.65 compared to pH 6.70, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the sufD, yidE, xylE, rlmM, mscL and dosC genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes sufD restored E. coli wild-type levels of acid tolerance, suggesting a possible role in acid homeostasis. Recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this bacterium.Chia-wei WuThomas SpikeDawn M. KlingemanMiguel RodriguezVirgil R. BremerSteven D. BrownNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chia-wei Wu Thomas Spike Dawn M. Klingeman Miguel Rodriguez Virgil R. Bremer Steven D. Brown Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
description	Abstract Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60–5.65, which is lower than has been reported previously. Wild type and acid tolerant strains digested corn stover with equal efficiency in batch culture at low pH. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.10 and 5.65 compared to pH 6.70, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the sufD, yidE, xylE, rlmM, mscL and dosC genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes sufD restored E. coli wild-type levels of acid tolerance, suggesting a possible role in acid homeostasis. Recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this bacterium.
format	article
author	Chia-wei Wu Thomas Spike Dawn M. Klingeman Miguel Rodriguez Virgil R. Bremer Steven D. Brown
author_facet	Chia-wei Wu Thomas Spike Dawn M. Klingeman Miguel Rodriguez Virgil R. Bremer Steven D. Brown
author_sort	Chia-wei Wu
title	Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
title_short	Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
title_full	Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
title_fullStr	Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
title_full_unstemmed	Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85
title_sort	generation and characterization of acid tolerant fibrobacter succinogenes s85
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b6f86f4e710a42288a69e44db6e8bb98
work_keys_str_mv	AT chiaweiwu generationandcharacterizationofacidtolerantfibrobactersuccinogeness85 AT thomasspike generationandcharacterizationofacidtolerantfibrobactersuccinogeness85 AT dawnmklingeman generationandcharacterizationofacidtolerantfibrobactersuccinogeness85 AT miguelrodriguez generationandcharacterizationofacidtolerantfibrobactersuccinogeness85 AT virgilrbremer generationandcharacterizationofacidtolerantfibrobactersuccinogeness85 AT stevendbrown generationandcharacterizationofacidtolerantfibrobactersuccinogeness85
_version_	1718388727766581248

Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85

Ejemplares similares