Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.

Due to insufficient morphological diagnostic characters in larval fishes, it is easy to misidentify them and difficult to key to the genus or species level. The identification results from different laboratories are often inconsistent. This experiment aims to find out, by applying DNA barcoding, how...

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Autores principales:	Hui-Ling Ko, Yu-Tze Wang, Tai-Sheng Chiu, Ming-An Lee, Ming-Yih Leu, Kuang-Zong Chang, Wen-Yu Chen, Kwang-Tsao Shao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b6fc27f7cf5d469c9773c43c212e9c8e
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spelling	oai:doaj.org-article:b6fc27f7cf5d469c9773c43c212e9c8e2021-11-18T07:59:17ZEvaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.1932-620310.1371/journal.pone.0053451https://doaj.org/article/b6fc27f7cf5d469c9773c43c212e9c8e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23382845/?tool=EBIhttps://doaj.org/toc/1932-6203Due to insufficient morphological diagnostic characters in larval fishes, it is easy to misidentify them and difficult to key to the genus or species level. The identification results from different laboratories are often inconsistent. This experiment aims to find out, by applying DNA barcoding, how inconsistent the identifications can be among larval fish taxonomists. One hundred morphotypes of larval fishes were chosen as test specimens. The fishes were collected with either larval fish nets or light traps in the northern, southern and northwestern waters of Taiwan. After their body lengths (SL) were measured and specimen photos were taken, all specimens were delivered, in turn, to five laboratories (A-E) in Taiwan to be identified independently. When all the results were collected, these specimens were then identified using COI barcoding. Out of a total of 100 specimens, 87 were identified to the family level, 79 to the genus level and 69 to the species level, based on the COI database currently available. The average accuracy rates of the five laboratories were quite low: 80.1% for the family level, 41.1% for the genus level, and 13.5% for the species level. If the results marked as "unidentified" were excluded from calculations, the rates went up to 75.4% and 43.7% for the genus and species levels, respectively. Thus, we suggest that larval fish identification should be more conservative; i.e., when in doubt, it is better to key only to the family and not to the genus or species level. As to the most misidentified families in our experiment, they were Sparidae, Scorpaenidae, Scombridae, Serranidae and Malacanthidae. On the other hand, Mene maculata and Microcanthus strigatus were all correctly identified to the species level because their larvae have distinct morphology. Nevertheless, barcoding remains one of the best methods to confirm species identification.Hui-Ling KoYu-Tze WangTai-Sheng ChiuMing-An LeeMing-Yih LeuKuang-Zong ChangWen-Yu ChenKwang-Tsao ShaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53451 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui-Ling Ko Yu-Tze Wang Tai-Sheng Chiu Ming-An Lee Ming-Yih Leu Kuang-Zong Chang Wen-Yu Chen Kwang-Tsao Shao Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.
description	Due to insufficient morphological diagnostic characters in larval fishes, it is easy to misidentify them and difficult to key to the genus or species level. The identification results from different laboratories are often inconsistent. This experiment aims to find out, by applying DNA barcoding, how inconsistent the identifications can be among larval fish taxonomists. One hundred morphotypes of larval fishes were chosen as test specimens. The fishes were collected with either larval fish nets or light traps in the northern, southern and northwestern waters of Taiwan. After their body lengths (SL) were measured and specimen photos were taken, all specimens were delivered, in turn, to five laboratories (A-E) in Taiwan to be identified independently. When all the results were collected, these specimens were then identified using COI barcoding. Out of a total of 100 specimens, 87 were identified to the family level, 79 to the genus level and 69 to the species level, based on the COI database currently available. The average accuracy rates of the five laboratories were quite low: 80.1% for the family level, 41.1% for the genus level, and 13.5% for the species level. If the results marked as "unidentified" were excluded from calculations, the rates went up to 75.4% and 43.7% for the genus and species levels, respectively. Thus, we suggest that larval fish identification should be more conservative; i.e., when in doubt, it is better to key only to the family and not to the genus or species level. As to the most misidentified families in our experiment, they were Sparidae, Scorpaenidae, Scombridae, Serranidae and Malacanthidae. On the other hand, Mene maculata and Microcanthus strigatus were all correctly identified to the species level because their larvae have distinct morphology. Nevertheless, barcoding remains one of the best methods to confirm species identification.
format	article
author	Hui-Ling Ko Yu-Tze Wang Tai-Sheng Chiu Ming-An Lee Ming-Yih Leu Kuang-Zong Chang Wen-Yu Chen Kwang-Tsao Shao
author_facet	Hui-Ling Ko Yu-Tze Wang Tai-Sheng Chiu Ming-An Lee Ming-Yih Leu Kuang-Zong Chang Wen-Yu Chen Kwang-Tsao Shao
author_sort	Hui-Ling Ko
title	Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.
title_short	Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.
title_full	Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.
title_fullStr	Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.
title_full_unstemmed	Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.
title_sort	evaluating the accuracy of morphological identification of larval fishes by applying dna barcoding.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b6fc27f7cf5d469c9773c43c212e9c8e
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Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding.

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