Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1)
Abstract It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Gala...
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Nature Portfolio
2021
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oai:doaj.org-article:b6fe687b75134f76aa55524c052b7bd72021-11-28T12:21:16ZDual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1)10.1038/s41598-021-02029-02045-2322https://doaj.org/article/b6fe687b75134f76aa55524c052b7bd72021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02029-0https://doaj.org/toc/2045-2322Abstract It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Galactins1 (MV-gal1) with the ability of glycan binding to screen every spiked virus. MV are the native vesicles which may have the gal-1 receptor. Gal-1 is the one of lectin receptor which can bind to glycan. After dropping the MV-gal1 on the SCPE/GNP, the sensor is turned on due to the increased electrochemical exchange with [Fe(CN)6]−3/−4 probe. Dropping the viral particles of SARS-CoV-2 cause to turn off the sensor with covering the sugar bond (early screening). Then, with the addition of Au/Antibody-SARS-CoV-2 on the MV-gal1@SARS-CoV-2 Antigen, the sensor is turned on again due to the electrochemical amplifier of AuNP (specific detection).For the first time, our sensor has the capacity of screening of any spike virus, and the specific detection of COVID-19 (LOD: 4.57 × 102 copies/mL) by using the natural bed-receptor and a specific antibody in the point of care test.E. GhazizadehAli NeshastehrizAli Dehghani FiroozabadiMohammad Kaji YazdiEsmail Saievar-IranizadSamira EinaliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
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Medicine R Science Q E. Ghazizadeh Ali Neshastehriz Ali Dehghani Firoozabadi Mohammad Kaji Yazdi Esmail Saievar-Iranizad Samira Einali Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1) |
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Abstract It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Galactins1 (MV-gal1) with the ability of glycan binding to screen every spiked virus. MV are the native vesicles which may have the gal-1 receptor. Gal-1 is the one of lectin receptor which can bind to glycan. After dropping the MV-gal1 on the SCPE/GNP, the sensor is turned on due to the increased electrochemical exchange with [Fe(CN)6]−3/−4 probe. Dropping the viral particles of SARS-CoV-2 cause to turn off the sensor with covering the sugar bond (early screening). Then, with the addition of Au/Antibody-SARS-CoV-2 on the MV-gal1@SARS-CoV-2 Antigen, the sensor is turned on again due to the electrochemical amplifier of AuNP (specific detection).For the first time, our sensor has the capacity of screening of any spike virus, and the specific detection of COVID-19 (LOD: 4.57 × 102 copies/mL) by using the natural bed-receptor and a specific antibody in the point of care test. |
format |
article |
author |
E. Ghazizadeh Ali Neshastehriz Ali Dehghani Firoozabadi Mohammad Kaji Yazdi Esmail Saievar-Iranizad Samira Einali |
author_facet |
E. Ghazizadeh Ali Neshastehriz Ali Dehghani Firoozabadi Mohammad Kaji Yazdi Esmail Saievar-Iranizad Samira Einali |
author_sort |
E. Ghazizadeh |
title |
Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1) |
title_short |
Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1) |
title_full |
Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1) |
title_fullStr |
Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1) |
title_full_unstemmed |
Dual electrochemical sensing of spiked virus and SARS-CoV-2 using natural bed-receptor (MV-gal1) |
title_sort |
dual electrochemical sensing of spiked virus and sars-cov-2 using natural bed-receptor (mv-gal1) |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/b6fe687b75134f76aa55524c052b7bd7 |
work_keys_str_mv |
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